关键词: 人肾小球系膜细胞, 细胞原代培养, 肾皮质组织块法, 优生选择法, 泌尿系统组织工程

Abstract:

BACKGROUND: Primary culture of glomerular mesangial cells was less achievement ratio, short survival time, and less passage times. In particular, extraction of renal glomerulus remains difficult for culturing highly pure mesangial cell.
OBJECTIVE: To establish a more simple, high successful rate and good reproducibility method of human mesangial cells in primary culture.
METHODS: Kidneys isolated from induction of labor with water bag voluntary were cut into pieces, and human mesangial cells were cultured with eugenic selection methods. Morphology was observed using inverted phase contrast microscope and transmission electron microscope, cell phenotype was detected using immunohistochemical method, and vimentin expression was observed using laser scanning confocal microscope.
RESULTS AND CONCLUSION: The cultured mesangial cells were fusiform-shaped, irregular star-shaped, and slender. Organelle was rich in cytoplasm, cell process was clear, and microvillus was observed on the cell membrane. The cells expressed α-actin, myosin, vimentin, desmin but not expressed cytokeratin and VIII factor. Laser scanning confocal microscope demonstrated that vimentin expression was positive and had the characteristics of fiber bundles. This suggested that the cultured intercapillary cells were coincidence with the characteristics of mesangial cell. The renal cortical tissue combined eugenic selection method was a simple and efficient method to culture human mesangial cells.