中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (51): 8856-8862.doi: 10.3969/j.issn.2095-4344.2013.51.012

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米钛酸钙对鼠成骨细胞增殖与分化的影响

翁志强1,孙  敏2,唐旭炎3,吴占敖2,端礼荣4,姜  涛2   

  1. 1解放军南京军区南京总医院门诊,江苏省南京市  210002;2解放军359医院口腔科,江苏省镇江市  212001;3安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,安徽省合肥市  230032;4江苏大学基础与医学技术学院,江苏省镇江市  212013
  • 出版日期:2013-12-17 发布日期:2013-12-17
  • 通讯作者: 唐旭炎,副教授,副主任医师,硕士生导师,安徽医科大学附属口腔医院口腔修复科,安徽省合肥市 230032 txy8302@hotmail.com
  • 作者简介:翁志强,男,1963年生,汉族,解放军第四军医大学毕业,副主任医师,主要从事种植体表面改性研究。 wenzhiqiang9898@sina.com
  • 基金资助:

    南京军区医学科技创新项目重点课题(联卫2013-71号)*

Nano-calcium titanate effects on rat osteoblast proliferation and differentiation

Weng Zhi-qiang1, Sun Min2, Tang Xu-yan3, Wu Zhan-ao2, Duanli Rong4, Jiang Tao2   

  1. 1 Outpatient Department, Nanjing General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing  210002, Jiangsu Province, China; 2 Department of Stomatology, the 359 Hospital of Chinese PLA, Zhenjiang  212001, Jiangsu Province, China; 3 Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei  230032, Anhui Province, China; 4 School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
  • Online:2013-12-17 Published:2013-12-17
  • Contact: Tang Xu-yan, Associate professor, Associate chief physician, Master’s supervisor, Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, Anhui Province, China txy8302@hotmail.com
  • About author:Weng Zhi-qiang, Associate chief physician, Outpatient Department, Nanjing General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing 210002, Jiangsu Province, China wenzhiqiang9898@sina.com
  • Supported by:

    Medical Science and Technology Innovation Project of Nanjing Military Region, No. 2013-71*

摘要:

背景:以不同修饰剂制备的纳米钛酸钙具有良好的骨传导性、生物相容性及生物活性。
目的:观察纳米钛酸钙材料对成骨细胞增殖、分化的影响。
方法:分别以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备纳米钛酸钙,同时制备无修饰剂的纳米钛酸钙。将制备的4组纳米钛酸钙分别配制为0.1,1.0,10 g/L的浸提液,MTT方法检测材料浸提液对乳鼠成骨细胞增殖及碱性磷酸酶活性的影响,以正常培养的细胞为对照。
结果与结论:以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备的纳米钛酸钙,无修饰剂的纳米钛酸钙在1.0,10 g/L质量浓度下可明显促进成骨细胞的增殖;上述4组纳米钛酸钙浸提液在1.0,10 g/L质量浓度下,培养3,6,9 d的细胞A值也高于对照组(P < 0.05,P < 0.01),培养6,9 d的细胞碱性磷酸酶活性值均高于对照组(P < 0.05,P < 0.01)。表明以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠、无修饰剂制备的钛酸钙对成骨细胞无毒性作用,可促进成骨细胞的增殖和分化。


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 纳米生物材料, 纳米, 钛酸钙, 成骨细胞, 细胞增殖, 细胞分化, 其他基金

Abstract:

BACKGROUND: Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility.
OBJECTIVE: To observe the influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts.
METHODS: Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. All the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast.
RESULTS AND CONCLUSION: The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L) for 3, 6, 9 days exceeded the contrast (P < 0.05, P < 0.01), as well as the alkaline phosphatase activity (P < 0.05, P < 0.01). Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: biomaterials, nanoparticles, osteoblasts, cell proliferation, mouth

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