中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5735-5738.doi: 10.3969/j.issn.1673-8225.2011.31.008

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

化学去细胞同种异体神经移植修复大鼠骶1神经缺损

李航旭1，邹  练2，刘德忠2，张晓毅2，张子良2   

  1. 1辽宁医学院研究生院，辽宁省锦州市 121000
    2解放军第二炮兵总医院泌尿外科，北京市 100088
  • 收稿日期:2011-01-13 修回日期:2011-03-28 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 邹练，医学博士，教授，博士生导师，解放军第二炮兵总医院泌尿外科，北京市 100088 lianzou@126.com
  • 作者简介:李航旭★，男，1983年生，辽宁省锦州市人，汉族，2008年辽宁医学院毕业，硕士，辽宁医学院附属第三医院医师，主要从事神经泌尿学、组织工程方面研究。 lihangxu913@163.com

Chemically acellularized nerve allografts for repair of rat S1 nerve defects   

Li Hang-xu1, Zou Lian2, Liu De-zhong2, Zhang Xiao-yi2, Zhang Zi-liang2   

  1. 1Liaoning Medical University Postgraduate School, Jinzhou 121000, Liaoning Province, China
    2Department of Urology, General Hospital of Second Artillery Forces of Chinese PLA, Beijing   100088, China
  • Received:2011-01-13 Revised:2011-03-28 Online:2011-07-30 Published:2011-07-30
  • Contact: Zou Lian, M.D. Professor, Doctoral supervisor, Department of Urology, General Hospital of Second Artillery Forces of Chinese PLA, Beijing 100088, China lianzou@126.com
  • About author:Li Hang-xu★, Master, Resident physician Liaoning Medical University Postgraduate School, Jinzhou 121000, Liaoning Province, China lihangxu913@163. com

PDF

318

被引次数

9

摘要：

背景：自体神经移植是修复周围神经损伤中最常用的方法。
目的：探讨化学去细胞神经修复大鼠骶1神经缺损的效果，以期寻找修复周围神经缺损较为理想的方法和材料。
方法：取SD大鼠骶1神经，采用化学去细胞法处理大鼠骶1神经的免疫原性成分，使其成为去细胞神经。将Wistar大鼠右侧骶1神经制成1 cm缺损模型，用SD大鼠去细胞同种异体神经移植修复大鼠骶1神经的缺损。
结果与结论：与术前相比，术后8周逼尿肌漏尿点降低，膀胱最大容积和膀胱顺应性升高(P < 0.05)。神经纤维细丝蛋白染色结果显示，右侧经同种异体神经移植修复的神经吻合口断端被染成绿色，神经纤维排列整齐分布均一，再生神经轴突长入远端神经。左侧正常神经形态均一，神经纤维排列整齐分布均一未见轴突长入远端神经。结果表明，化学去细胞同种异体神经的抗原性明显降低，可修复高等哺乳类动物的周围神经损伤。

关键词: 周围神经, 同种异体移植, 神经缺损模型, 尿动力学, 大鼠

Abstract:

BACKGROUND: Autologous nerve transplantation is the most common method for repair of peripheral nerve injury.
OBJECTIVE: To investigate the efficacy of chemically acellularized nerve allografts for repair of rat S1 nerve defects and to search the ideal methods and materials for repair of peripheral nerve injury.
METHODS: SD rat S1 nerve was taken and chemically acellularized to remove the immunogenic component. 1 cm long incision was made on the right S1 in SD rats and bridged with the nerve allograft.
RESULTS AND CONCLUSION: Compared with prior to surgery, the urinary leakage site of the detrusor muscle was lowered, the maximum volume and compliance of the bladder were significantly increased (P < 0.05) at 8 weeks after surgery. Neurofilament staining showed that on the experimental (right) side, neural anastomotic stoma was stained green, nerve fibers were orderly distributed, and regenerated axons grew towards distal nerve; on the left side, nerve morphology was normal, nerve fibers were orderly arranged, and axons did not grow toward distal nerve. These findings suggest that chemically acellularized nerve allografts with obviously decreased antigenicity can repair peripheral nerve injury in high grade mammals.

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