中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5701-5706.doi: 10.3969/j.issn.1673-8225.2011.31.001

• 移植与免疫 transplantation and Immunology •    下一篇

复合组织及血清肿瘤坏死因子α、白细胞介素10表达与大鼠喉移植急性排斥反应的相关性

袁  牧1，陈  刚2，赵  华2，荆建军2   

  1. 1解放军新疆军区机关门诊部，新疆维吾尔自治区乌鲁木齐市  830002
    2解放军兰州军区乌鲁木齐总医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市 830000
  • 收稿日期:2011-03-04 修回日期:2011-06-19 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 陈刚，博士，主治医师，解放军兰州军区乌鲁木齐总医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市 830000
  • 作者简介:袁牧，男，1971年出生，汉族，陕西省兴平市人，1993年解放军第三军医大学毕业，主治医师，主要从事全科医学方面研究。

Correlation of tumor necrosis factor-alpha and interleukin-10 expression to acute rejection in complex tissue and serum of rats after laryngeal transplantation

Yuan Mu1, Chen Gang2, Zhao Hua2, Jing Jian-jun2   

  1. 1Outpatient Department, Xinjiang Military Area Command of Chinese PLA, Urumqi  830002, Xinjiang Uygur Autonomous Region, China
    2Department of Otorhinolaryngology, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-03-04 Revised:2011-06-19 Online:2011-07-30 Published:2011-07-30
  • Contact: Chen Gang, Doctor, Attending physician, Department of Otorhinolaryngology, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumqi 830000, Xinjiang Uygur Autonomous Region, China chengangxf@sina. com
  • About author:Yuan Mu, Attending physician, Outpatient Department, Xinjiang Military Area Command of Chinese PLA, Urumqi 830002, Xinjiang Uygur Autonomous Region, China 13039493151@163.com

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摘要：

背景：喉移植后致炎细胞因子(肿瘤坏死因子α、γ-干扰素等)和抗炎细胞因子(白细胞介素10、白细胞介素4等)之间的相互作用会发生哪些变化呢？
目的：观察肿瘤坏死因子α、白细胞介素10在大鼠喉移植急性排斥反应期移植喉组织的不同部位和血清中的表达变化，评价其血清水平在预测急性排斥反应中的作用。
方法：进行Wistar→SD大鼠喉移植，移植后依照注射环孢霉素A剂量不同随机分为3组：0 mg组、5 mg组及10 mg组，以未进行喉移植的SD大鼠为正常对照组。
结果与结论：各组移植后第3，7，11天肿瘤坏死因子α、白细胞介素10血清浓度的变化与移植后各相应时间点黏膜上皮及黏膜下层组织中其表达水平的变化均呈正相关。提示，供体喉的高抗原性主要集中于喉的黏膜上皮层及黏膜下层组织；血清肿瘤坏死因子α和白细胞介素10的浓度可以作为预测喉移植术后急性排斥反应的指标。

关键词: 肿瘤坏死因子α, 白细胞介素10, 喉移植, 排斥反应, 大鼠

Abstract:

BACKGROUND: It remains unclear whether proinflammatory factor, such as tumor necrosis factor-α (TNF-α), γ-interferon and anti-inflammatory cytokine, such as interleukin-10 (IL-10), interleukin-4 (IL-4) levels can change after laryngeal transplantation.
OBJECTIVE: To explore the expression of TNF-α and IL-10 in different expressive tissue layers and its relationship during the acute rejection episodes, and to evaluate the role of TNF-α and IL-10 levels in serum for prediction of early acute rejection after laryngeal transplantation.
METHODS: Laryngeal heterotopic transplantations were performed in Wistar and Sprague-Dawley rats. According to different dosages of immunodepressant, all recipients were divided into three groups: Group 0 mg, Group 5 mg, and Group 10 mg. Untreated Sprague-Dawley rats served as normal control group.
RESULTS AND CONCLUSION: Changes in serum TNF-α and IL-10 levels at postoperation days 3, 7 and 11 were positively correlated with these expressions in the epithelium mucosa and submucosa at various time points after transplantation. These indicate that the high-antigenicity of graft mainly concentrates on the layers of mucosa and submucosa. TNF-α and IL-10 concentrations can serve as indexes for predicting acute rejection after laryngeal transplantation.

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