中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (29): 5389-5392.doi: 10.3969/j.issn.1673-8225.2011.29.017

• 药物控释材料 drug delivery materials • 上一篇    下一篇

几丁糖对大鼠股动脉吻合口通畅率的影响

王亚峰1，袁正江1，赵爱娟1，张新营1，梁  豪2   

  1. 河南科技大学第一附属医院，1显微外科，2泌尿外科，河南省洛阳市   471003
  • 收稿日期:2010-12-09 修回日期:2011-02-23 出版日期:2011-07-16 发布日期:2011-07-16
  • 通讯作者: 袁正江，主任医师，河南科技大学第一附属医院显微外科，河南省洛阳市 471003 luoyangwangyafeng@163.com
  • 作者简介:王亚峰★，男，1979年生，河南省巩义市人，汉族，河南科技大学在读硕士，医师，主要从事断指再植、足趾移植的功能康复治疗研究。

Chitosan effect on femoral artery anastomosis patency rate in rats

Wang Ya-feng1, Yuan Zheng-jiang1, Zhao Ai-juan1, Zhang Xin-ying1, Liang Hao2   

  1. 1Department of Microsurgery, 2Department of Urology, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang  471003, Henan Province, China
  • Received:2010-12-09 Revised:2011-02-23 Online:2011-07-16 Published:2011-07-16
  • Contact: Yuan Zheng-jiang, Chief physician, Department of Microsurgery, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China luoyangwangyafeng@163.com
  • About author:Wang Ya-feng★, Studying for master’s degree, Physician, Department of Microsurgery, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China

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摘要：

背景：几丁糖在预防术后组织粘连方面疗效确切，但在断指再植术后能否应用，其对血管吻合口的愈合是否有影响，国内外文献较少提及。
目的：观察医用几丁糖对成年大鼠股动脉吻合口通畅率的影响。
方法：离断SD大鼠双侧股动脉，左侧为实验组，右侧为对照组，显微镜下吻合双侧股动脉，所有吻合动脉即时通畅率达100%，且为同一人操作，吻合后，实验组吻合口周围均匀涂布医用几丁糖1滴，对照组不涂。分别观察术后7，14，21，28 d大鼠吻合口的通畅情况，吻合口口径、吻合口周围有无感染、周围粘连情况及病理切片观察吻合口内皮细胞覆盖情况。
结果与结论：术前、术后吻合口管径无明显变化，实验组和对照组吻合口周围均无明显感染，实验组吻合口周围粘连较对照组轻，吻合口总体通畅率实验组为96%，对照组为89%，差异无显著性意义。术后各个时间段实验组、对照组内皮细胞覆盖情况差异无显著性意义。提示医用几丁糖的放置不影响大鼠股动脉吻合口的总体通畅率。

关键词: 几丁糖, 股动脉, 通畅率, 大鼠, 吻合

Abstract:

BACKGROUND: Chitosan can effectively prevent postoperative tissue adhesion. But in amputation, whether replantation surgery has effects on vascular anastomosis is less reported.
OBJECTIVE: To study the medical effects of chitosan on adult rat femoral artery anastomosis patency rate. 
METHODS: Bilateral transection of the femoral artery was performed in SD rats, left side as the experimental group, and contralateral (right) as the control group. Anatomosis of bilateral femoral artery was performed under a microscope, all the immediate arterial anastomosis patency rate was 100%, and the same person operated. One drop of chitosan was covered surrounding the anatomosis site in the experiment group. After 7, 14, 21, 28 days, anastomotic patency was observed as well as anastomotic diameter, with or without infection around the stoma, adhesions around the anastomosis, anastomotic coverage of endothelial cells (biopsy).
RESULTS AND CONCLUSION: Preoperative and postoperative anastomotic diameter had no significant changes in the experimental group and control group without obvious infection around the stoma. The adhesion was slighter in the experimental group than in the control group. The overall anastomotic patency rate was 96% in the experimental group, and 89% in the control group. At various time periods after operation, endothelial cell coverage had no difference between the two groups. Chitosan does not affect the rat femoral artery anastomosis overall patency rate.

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