中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (29): 5367-5370.doi: 10.3969/j.issn.1673-8225.2011.29.012

• 药物控释材料 drug delivery materials • 上一篇    下一篇

生肌玉红胶原海绵修复创面损伤

严婷婷1，熊  猛1，周  勇2，姚  昶2   

  1. 1东南大学附属中大医院整形外科，江苏省南京市 210009
    2江苏省中医院普外科，江苏省南京市   210009
  • 收稿日期:2011-03-07 修回日期:2011-05-09 出版日期:2011-07-16 发布日期:2011-07-16
  • 通讯作者: 姚昶，副主任医师，江苏省中医院普外科，江苏省南京市 210009
  • 作者简介:严婷婷★，女，1982年生，安徽省淮南市人，汉族，东南大学医学院在读硕士，主要从事创面愈合研究。 yan_xiaomao@163.com
  • 基金资助:

    国家自然科学基金项目(30973756)，江苏省中医药领军人才项目(LJ200902)。

Sheng Ji Yu Hong collagen matrices for repair of skin wound

Yan Ting-ting1, Xiong Meng2, Zhou Yong2, Yao Chang2   

  1. 1Department of Plastic Surgery, Zhongda Hospital, Southeast University, Nanjing  210009, Jiangsu Province, China
    2Department of General Surgery, Jiangsu Hospital of Traditional Chinese Medicine, Nanjing  210009, Jiangsu Province, China
  • Received:2011-03-07 Revised:2011-05-09 Online:2011-07-16 Published:2011-07-16
  • Contact: Yao Chang, Associate chief physician, Department of General Surgery, Jiangsu Hospital of Traditional Chinese Medicine, Nanjing 210009, Jiangsu Province, China
  • About author:Yan Ting-ting★, Studying for master’s degree, Department of Plastic Surgery, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China yan_xiaomao@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973756*; Leading Talent Project of Traditional Chinese Medicine in Jiangsu Province, No. LJ200902*

PDF

404

被引次数

34

摘要：

背景：生肌玉红胶原海绵能显著促进创面愈合。
目的：观察生肌玉红胶原海绵对兔皮肤创面愈合的影响。
方法：在新西兰白兔背部制造3处全层皮肤缺损创面，分别以生肌玉红胶原海绵、贝复剂(重组碱性成纤维细胞生长因子外用溶液)、生理盐水修复。观察各组创面愈合时间、创面愈合率，检测创面修复组织内成纤维细胞数、血红素氧化酶1、转化生长因子β1 mRNA与蛋白及增殖细胞核抗原蛋白表达。
结果与结论：与贝复剂、生理盐水比较，生肌玉红胶原海绵可显著促进成纤维细胞增殖(P < 0.05)，增加创面血红素氧化酶1、转化生子因子β1及增殖细胞核抗原的表达(P < 0.05)，提高创面愈合率(P < 0.05)，缩短创面愈合时间(P < 0.05)。证实生肌玉红胶原海绵能够促进创面修复，其作用机制可能是通过提高创面血红素氧化酶1、转化生长因子β1表达促进细胞增殖，增加成纤维细胞等创面修复细胞。

关键词: 生肌玉红胶原海绵, 创面愈合, 血红素氧化酶1, 转化生长因子β1, 增殖细胞核抗原

Abstract:

BACKGROUND: Sheng Ji Yu Hong collagen matrices can obviously promote wound healing.
OBJECTIVE: To observe the effect of Sheng Ji Yu Hong collagen matrices on rabbit skin wound healing.
METHODS: Three full-thickness skin wounds were made and repaired with Sheng Ji Yu Hong collagen matrices, BFGF agent, normal saline solution, separately. Healing time, healing ratio, number of fibroblasts, hemeoxygenase-1 level, transforming growth factor β1 mRNA and protein expression, and proliferating cell nuclear antigen expression were observed.
RESULTS AND CONCLUSION: Sheng Ji Yu Hong could promote the proliferation of fibroblasts more remarkably than BFGF agent and normal saline (P < 0.05), increase expressions of hemeoxygenase-1 level, transforming growth factor β1 and proliferating cell nuclear antigen (P < 0.05), elevate healing rate (P < 0.05), and shorten healing time (P < 0.05). Sheng Ji Yu Hong collagen matrices can promote skin wound healing obviously, and the mechanism maybe relates to elevate the expression of hemeoxygenase-1 level and transforming growth factor β1, and preserve the activity of prosthetic cells.

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