中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5229-5232.doi: 10.3969/j.issn.1673-8225.2011.28.025

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

改构型酸性成纤维细胞生长因子干预慢性衰老模型大鼠脑及肝组织和血液ATP酶的活力

黄俊杰1，王彩冰1，黄丽娟1，赵善民1，何显教1，黄彦峰1，梁祚仁1，李倩茗2，黄巨恩3   

  1. 1右江民族医学院应用生理研究室，广西壮族自治区百色市 533000；广西医科大学，2生理学教研室，3组胚学教研室，广西壮族自治区南宁市 530021
  • 收稿日期:2011-02-09 修回日期:2011-05-14 出版日期:2011-07-09 发布日期:2011-07-09
  • 作者简介:黄俊杰★，男，1970年生，广西壮族自治区平果人，壮族，2007年广西医科大学毕业，硕士，副教授，主要从事神经生理与神经疾病方面的研究。 moke68@163.com
  • 基金资助:

    课题受广西教育厅科研项目资助，编号：桂教科研[2007]34号(200707LX136)，项目名称：bFGF对慢性衰老模型大鼠神经细胞保护作用的研究。

Effects of modified acidic fibroblast growth factor on the ATPase activities in blood, brain and liver tissue of chronic aging model rats

Huang Jun-jie1, Wang Cai-bing1, Huang Li-juan1, Zhao Shan-min1, He Xian-jiao1, Huang Yan-feng1, Liang Zuo-ren1, Li Qian-ming2, Huang Ju-en3   

  1. 1Department of Applied Physiology, Youjiang Medial College for Nationalities, Baise  533000, Guangxi Zhuang Autonomous Region, China; 2Department of Physiology, 3Department of Histology and Embryology, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-02-09 Revised:2011-05-14 Online:2011-07-09 Published:2011-07-09
  • About author:Huang Jun-jie★, Master, Associate professor, Department of Applied Physiology, Youjiang Medial College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China moke68@163.com
  • Supported by:

    Science and Technology Fund by Education Ministry of Guangxi Zhuang Autonomous Region, No. 200707LX136*

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被引次数

1

摘要：

背景：有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子，但其抗衰老作用至今尚未有报道。
目的：观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力的影响。
方法：将Wistar大鼠采用皮下注射D-半乳糖建立衰老模型，建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子组，另设正常对照组。改构型酸性成纤维细胞生长因子组按12 µg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子，生理盐水对照组肌肉注射等量的生理盐水，模型组不干预。
结果与结论：与正常对照组相比，模型组大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显著降低(P < 0.01)。改构型酸性成纤维细胞生长因子组脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显高于模型组和生理盐水对照组(P < 0.05)。结果提示，改构型酸性成纤维细胞生长因子能提高衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力，具有延缓衰老作用。

关键词: 改构型酸性成纤维细胞生长因子, D-半乳糖, 衰老, ATP酶活力, 组织工程

Abstract:

BACKGROUND: Modified acidic fibroblast growth factor (maFGF) is a multifunctional grows factor, but its anti-aging actions remains unclear.
OBJECTIVE: To study the effects of maFGF on the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in brain tissue, liver tissue and red blood cells of D-galactose-induced aging model rats.
METHODS: The aging model in Wistar rats was established by subcutaneous injection of D-galactose. The successful models were randomly divided into model group, normal saline group and maFGF treatment group. Rats in the latter two groups received a 12-µg/kg intramuscular injection of normal saline and maFGF, respectively. Model group received no treatment. In addition, normal control group was set.
RESULTS AND CONCLUSION: The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in brain tissue, liver tissue and red blood cells were significantly decreased compared with normal control group (P < 0.01). After maFGF intervention, the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were significantly higher than those in the normal saline and model groups (P < 0.05). The maFGF can increase the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in brain tissue, liver tissue and red blood cells and delay the ageing in D-galactose-induced aging model rats.

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