中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5181-5184.doi: 10.3969/j.issn.1673-8225.2011.28.013

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

急性脊髓损伤模型大鼠白细胞介素1β和核因子κB的表达

宗少晖1，韦  波2，曾高峰2，赵玉玺3，熊春翔3    

  1. 1广西医科大学第一附属医院脊柱骨病外科，广西壮族自治区南宁市  530021；广西医科大学，2公共卫生学院，3研究生学院，广西壮族自治区南宁市  530021
  • 收稿日期:2010-12-15 修回日期:2011-02-22 出版日期:2011-07-09 发布日期:2011-07-09
  • 作者简介:宗少晖☆，男，1972年生，广西壮族自治区桂林市人，瑶族，2005年北京大学毕业，博士，副教授，主要从事脊柱脊髓疾病的临床治疗方面的研究。 xiaohui3008@ 126.com
  • 基金资助:

    广西医科大学博士启动基金(308010)，广西教育厅科研基金(桂教200710LX063)。

Expression of interleukin-1 β and nuclear factor kappa B in rat models of acute spinal cord injury

Zong Shao-hui1, Wei Bo2, Zeng Gao-feng2, Zhao Yu-xi3, Xiong Chun-xiang3   

  1. 1Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2School of Public Health, 3School of Postgraduate, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-12-15 Revised:2011-02-22 Online:2011-07-09 Published:2011-07-09
  • About author:Zong Shao-hui,☆ Doctor, Associate professor, Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaohui3008@126. com
  • Supported by:

    Ph.D Programs Foundation of Guangxi Medical University, No. 308010*; Science Research Foundation of Ministry of Education of Guangxi Zhuang Autonomous Region, Guijiao 200710LX063*

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摘要：

背景：在脊髓损伤后的继发性损伤过程中，白细胞介素1β参与刺激其他细胞因子和损伤介质的合成。
目的：观察白细胞介素1受体拮抗剂对急性脊髓损伤模型大鼠损伤脊髓白细胞介素1β与核因子κB表达的影响。
方法：采用改良Allen法建立SD大鼠急性脊髓损伤模型，造模后分别在损伤处敷含白细胞介素1受体拮抗剂或仅有生理盐水的明胶海绵，于脊髓损伤1，48，72 h取损伤段脊髓标本，免疫组织化学染色检测白细胞介素1β与核因子κB的表达。
结果与结论：经白细胞介素1受体拮抗剂治疗后，损伤脊髓组织白细胞介素1β和核因子κB的表达均显著降低。说明白细胞介素1受体拮抗剂可通过抑制白细胞介素1β和核因子κB的表达，减轻局部炎症反应，对急性脊髓损伤大鼠损伤段脊髓发挥保护作用。

关键词: 白细胞介素1受体拮抗剂, 脊髓损伤, 白细胞介素1&beta, 核因子&kappa, B, 大鼠

Abstract:

BACKGROUND: During the process of secondary spinal cord injury, interleukin-1β participates in stimulation of other cytokines and synthesis of injury medium.
OBJECTIVE: To investigate the effects of interleukin-1 receptor antagonist on the expression of interleukin-1β and nuclear factor-κB in rat models of acute spinal cord injury.
METHODS: Sprague-Dawley rat models of acute spinal cord injury were established by modified Allen method. After modeling, the injured spinal cord tissue was spread gelatin sponge containing interleukin-1 receptor antagonist or normal saline. At 1, 48, and 72 hours postoperatively, the injured spinal cord tissue samples were harvested for detection of interleukin-1β and nuclear factor κB by immunohistochemical method.
RESULTS AND CONCLSUION: After interleukin-1 receptor antagonist treatment, interleukin-1β and nuclear factor κB expression was greatly decreased. These findings suggest that interleukin-1 receptor antagonist can alleviate local inflammatory reaction by inhibiting interleukin-1β and nuclear factor κB expression, exerting protective effects on injured spinal cord segment in rat models of acute spinal cord injury.

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