中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5161-5164.doi: 10.3969/j.issn.1673-8225.2011.28.008

• 骨组织构建 bone tissue construction • 上一篇    下一篇

仙灵骨葆对骨质疏松性骨折骨痂血管形成的影响

田发明1，张  柳1,2，骆  阳2，宋亚琪1，矫  傲1，程  潭2   

  1. 1河北联合大学附属医院骨外科，河北省唐山市  063000
    2河北医科大学研究生院，河北省石家庄市  050017
  • 收稿日期:2011-01-15 修回日期:2011-03-25 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 张柳，教授，主任医师，博士生导师，河北联合大学附属医院骨外科，河北省唐山市 063000；河北医科大学研究生院，河北省石家庄市 050017 zhliu130@sohu.com
  • 作者简介:田发明☆，男，1980年生，河北省保定市人，2011年河北医科大学毕业，博士，主要从事骨质疏松的基础与临床研究。 tfm9911316@163.com

Xianlinggubao promotes vascular formation in osteoporotic fracture callus

Tian Fa-ming1, Zhang Liu1, 2, Luo Yang2, Song Ya-qi1, Jiao Ao1, Cheng Tan 2   

  1. 1Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan  063000, Hebei Province, China
    2Postgraduate School, Hebei Medical University, Shijiazhuang  050017, Hebei Province, China
  • Received:2011-01-15 Revised:2011-03-25 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhang Liu, Professor, Chief physician, Doctoral supervisor, Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China; Postgraduate School, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China zhliu130@sohu.com
  • About author:Tian Fa-ming☆, Doctor, Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China tfm9911316@163.com

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656

被引次数

21

摘要：

背景：骨质疏松对骨折愈合早期的影响尚存争议，仙灵骨葆对骨质疏松性骨折愈合的影响及其机制尚有待深入研究。
目的：观察骨质疏松对大鼠股骨干骨折愈合的影响，以及仙灵骨葆对骨质疏松性骨折愈合的作用。
方法：将50只雌性12周龄Sprague-Dawley大鼠随机分成5组：假手术组、骨折组、去卵巢组、去卵巢+骨折组及治疗组，后3组切除大鼠双侧卵巢制备去卵巢模型，骨折模型于去卵巢后4周制备，为股骨干中段横行骨折。治疗组在去卵巢及骨折基础上灌胃给予仙灵骨葆250 mg/(kg•d)。给药3周，取去卵巢+骨折组、骨折组和治疗组大鼠骨折侧标本行X射线摄像仪摄片后，测量其骨密度，苏木精-伊红染色观察骨痂组织的病理学改变并计数血管，免疫组织化学染色检测骨痂组织骨形态发生蛋白2的表达。
结果与结论：去卵巢处理后大鼠的骨密度、计算机X射线摄像仪摄片评分显著降低(P < 0.05)，仙灵骨葆可在一定程度上提高去卵巢后骨折大鼠的骨密度及X射线摄像仪摄片评分，但差异无显著性意义(P > 0.05)；仙灵骨葆可提高去卵巢后骨折大鼠骨痂组织的血管数量(P < 0.05)，但对骨形态发生蛋白2的表达无影响。提示，去卵巢后大鼠骨折早期愈合过程延迟，仙灵骨葆可促进骨质疏松大鼠骨折愈合早期血管的形成。

关键词: 仙灵骨葆, 骨质疏松, 骨折愈合, 血管计数, 骨形态发生蛋白2

Abstract:

BACKGROUND: Controversy still exists in impact of osteoporosis on early process of fracture healing, and the effect and related mechanism underlying Xianlinggubao (XLGB) on osteoporotic fracture healing need further study.
OBJECTIVE: To confirm the influence of osteoporosis on the healing of femoral shaft fracture and to investigate the effects of XLGB on osteoporotic fracture healing.
METHODS: Fifty female 12-week old Sprague-Dawley rats were randomly divided into 5 groups with 10 animals in each group: sham-surgery, fracture, ovariectomy (OVX), OVX + fracture and XLGB. OVX was performed in bilateral sides of rats. Transverse fracture model of femoral shaft was established at 4 weeks after OVX. Rats in XLGB treatment group were treated with 250 mg/kg XLGB daily for 3 weeks following the establishment of OVX and fracture. Rats in the OVX + fracture, fracture and XLGB treatment groups were harvested the femoral fracture samples for bone mineral density test by X-ray radiography. The pathological changes of callus tissues were detected with hematoxylin-eosin staining and blood vessels were counted. Immunohistochemical staining was applied to determine the expression of bone morphogenetic protein 2 in callus tissues.
RESULTS AND CONCLUSION: Bone mineral density test and computed X-ray radiography score showed a significantly reduction in the rats following OVX (P < 0.05). XLGB treatment could enhance the bone mineral density and X-ray radiography score in the OVX and fracture rats, with no significant difference (P > 0.05). XLGB treatment also increased the quantity of callus tissues compared with OVX + fracture and fracture groups (P < 0.05). No significant difference was observed of bone morphogenetic protein-2 expression among three groups. The ovariectomized rats show a delayed fracture healing process and XLGB could promote the early vascular formation of osteoporotic fracture healing.

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