中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5157-5160.doi: 10.3969/j.issn.1673-8225.2011.28.007

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌激素受体基因ERβ与小鼠成骨细胞的增殖和分化

鲁世金，张宏其，高其乐，郭超峰，唐明星   

  1. 中南大学湘雅医院脊柱外科， 湖南省长沙市  410008
  • 收稿日期:2011-04-09 修回日期:2011-05-18 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 张宏其，教授，芙蓉学者，博士生导师，科主任，中南大学湘雅医院脊柱外科，湖南省长沙市 410008 zhq9996@163. com
  • 作者简介:鲁世金☆，男，1967年生，中南大学湘雅医院在读博士，主治医师，主要从事脊柱外科临床及相关基础研究。 jzwklu@yahoo. com.cn
  • 基金资助:

    湖南省研究生科研创新项目资助(CX2010B101)。

Estrogen receptor beta inhibits the proliferation and differentiation of osteoblasts in mice

Lu Shi-jin, Zhang Hong-qi, Gao Qi-le, Guo Chao-feng, Tang Ming-xing   

  1. Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
  • Received:2011-04-09 Revised:2011-05-18 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhang Hong-qi, Professor, Lotus scholar, Doctoral supervisor, Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China zhq9996@163.com
  • About author:Lu Shi-jin☆, Studying for doctorate, Attending physician, Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China jzwklu@yahoo.com.cn
  • Supported by:

    Scientific Research Innovation Program  of Human Postgraduates, No.CX2010B101

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摘要：

背景：雌激素受体ERs表达于所有关系到骨形成和骨吸收的细胞成分中。
目的：观察雌激素受体ERβ对成骨细胞增殖、分化能力的调控作用。
方法：以小鼠成骨细胞株MC3T3-E1为对象，设立3组：实验组转染雌激素受体ERβ RNAi载体、阴性对照组转染ERL RNAi载体、空白对照组不进行转染，3组在相同条件下培养。采用MTT法绘制各组细胞的生长曲线；流式细胞仪分析各组细胞的细胞周期。
结果与结论：实验组成骨细胞的增殖能力明显高于空白对照组和阴性对照组，G1期细胞百分率明显少于空白对照组和阴性对照组，而S期和G2期细胞百分率明显高于空白对照组和阴性对照组(P均< 0.05)。根据雌激素受体ERβ沉默后检测的结果可以反向推断，ERβ的表达对成骨细胞的增殖、分化具有抑制作用。

关键词: 成骨细胞, 雌激素受体, RNAi, 增殖, 分化

Abstract:

BACKGROUND: Estrogen receptors (ERs) are expressed in cell components related to bone formation and resorption.
OBJECTIVE: To investigate the regulatory effects of Erβ on osteoblast proliferation and differentiation.
METHODS: The mouse osteoblastic cell lines MC3T3-E1 were divided into 3 groups: experimental group (transfected ERβRNAi vector), negative control (transfected ERL RNAi vector), and blank control (no transfection). Three groups of cells were cultured under the same conditions. In each group, cell growth curve was drawn using MTT; and the cell cycle was analyzed by flow cytometry.
RESULTS AND CONCLUSION: The cell proliferation in the experimental group was more significantly enhanced than in the negative control group or blank control group. The percentage of cells at G1-phase in the experimental group was significantly less than the other groups, while the percentage of cells at S-phase and G2-phase was significantly higher than the other groups (P < 0.05). According to test results of the ERβ silence, we can infer that the ERβ may inhibit the proliferation and differentiation of osteoblasts.

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