中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4988-4992.doi: 10.3969/j.issn.1673-8225.2011.27.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

猪脂肪干细胞与脱细胞真皮基质的生物相容性

赵宝成1，王振军1，韩加刚1，顾  蓓2，徐慧民3，喻学桥1   

  1. 1首都医科大学附属北京朝阳医院普外科，北京市  100020
    2中国医学科学院基础医学研究所，北京市  100730
    3潍坊市人民医院普外科，山东省潍坊市  261041
  • 收稿日期:2011-01-10 修回日期:2011-04-22 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 王振军，博士，主任医师，首都医科大学附属北京朝阳医院普外科，北京市 100020 wang3zj@sohu.com
  • 作者简介:赵宝成☆，男，1982年生，山东省临沂市人，汉族，首都医科大学在读博士，主要从事胃肠肿瘤及材料修补的研究。 zhaobc2010@163.com
  • 基金资助:

    北京市卫生系统高层次卫生技术人才(2009-1-03)；国家级新世纪百千万人才工程(08-009)。

Biocompatibility of procine adipose derived stem cells and acellular dermal matrix

Zhao Bao-cheng1, Wang Zhen-jun1, Han Jia-gang1, Gu Bei2, Xu Hui-min3, Yu Xue-qiao1   

  1. 1Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing  100020, China
    2Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing  100730, China
    3Department of General Surgery, Weifang People’s Hospital, Weifang  261041, Shandong Province, China
  • Received:2011-01-10 Revised:2011-04-22 Online:2011-07-02 Published:2011-07-02
  • Contact: Wang Zhen-jun, Doctor, Chief physician, Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China wang3zj@sohu.com
  • About author:Zhao Bao-cheng☆, Studying for doctorate, Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China Zhaobc2010@163.com
  • Supported by:

    Program for Outstanding Medical Field Leaders, Beijing, No. 2009-1-03*; the New Century National Hundred, Thousand and Ten Thousand Talent Project, No. 08-009*

PDF

342

被引次数

4

摘要：

背景：脱细胞真皮基质已广泛应用于器官或组织缺损的修补。
目的：观察猪脂肪源性干细胞与脱细胞真皮基质的相容性。
方法：酶消化法原代培养猪脂肪源性干细胞，利用流式细胞仪和多向诱导分化方法鉴定脂肪干细胞，将其与脱细胞真皮基质共培养。
结果与结论：实验成功培养出猪脂肪源性干细胞，流式细胞仪检测结果显示阳性表达CD44和CD105，不表达CD34和CD45；在诱导培养基条件下，可向脂肪细胞、成骨细胞分化。苏木精-伊红染色及扫描电镜发现干细胞能在脱细胞真皮基质表面黏附生长。脂肪源性干细胞与脱细胞真皮基质具有良好的相容性，将两者体外复合培养后，有望将复合物植入体内进行缺损组织、器官的修复。

关键词: 脱细胞真皮基质, 脂肪源性干细胞, 酶消化法, 培养, 生物相容性, 猪, 组织工程

Abstract:

BACKGROUND: Acellular dermal matrix has been widely used as a repair material in organ and tissue repair.
OBJECTIVE: To investigate the biocompatibility of procine adipose derived stem cells and acellular dermal matrix.
METHODS: Porcine adipose derived stem cells were obtained and cultured with collagenase digestion method. Flow cytometric analysis and adipogenic, octeogenic differentiation analysis were used to identify the stem cells.
RESULTS AND CONCLUSION: The stem cells isolated from subcutaneous adipose tissue were positive for CD44 and CD105, negative for CD34 and CD45. After cultured in mesenchymal stem cell adipogenic and octeogenic differentiation basal medium respectively for 3 weeks, the cells were positive for oil red O staining and alizarin bordeaux staining. Both hematoxylin-eosin staining and scanning electron microscopy analyses confirmed that adipose derived stem cells could adhere to acellular dermal matrix. Adipose derived stem cells have good biocompatibility with acellular dermal matrix. Adipose derived stem cells-seeded acellular dermal matrix can be used in tissue engineering as a mechanical reconstructive agent and as a biolayer of stem cells for targeted delivery.

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