中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4975-4978.doi: 10.3969/j.issn.1673-8225.2011.27.008

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

基因芯片分析成骨生长肽对OPG-/-小鼠骨髓间充质干细胞的作用

米尔萨力江•亚森1，郭常军1，费琴明1，陈统一1，崔大敷2   

  1. 1复旦大学附属中山医院骨科，上海市  200032
    2中国科学院上海生命科学研究院生物化学与细胞生物学研究所，上海市　200031
  • 收稿日期:2011-01-19 修回日期:2011-02-20 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 费琴明，博士，副教授，硕士生导师，复旦大学附属中山医院骨科，上海市 200032 fei.qinming@zs-hospital.sh.cn
  • 作者简介:米尔萨力江?亚森★，男，1985年生，新疆维吾尔自治区喀什市人，维吾尔族，复旦大学附属中山医院在读硕士，主要从事骨科研究。 mersali@sina.com

Effect of osteogenic growth peptide on gene expression changes in bone mesenchymal stem cells of OPG-/- mice: A Microarray analysis

Miersalijiang Ya-sen1, Guo Chang-jun1, Fei Qin-ming1, Chen Tong-yi1, Cui Da-fu2   

  1. 1Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai  200032, China
    2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, Shanghai  200031, China
  • Received:2011-01-19 Revised:2011-02-20 Online:2011-07-02 Published:2011-07-02
  • Contact: Fei Qin-ming, Ph.D., Associate professor, Master’s supervisor, Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China fei.qinming@zs-hospital.sh.cn
  • About author:Miersalijiang Ya-sen★, Studying for master’s degree, Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China mersali@sina.com

PDF

353

被引次数

4

摘要：

背景：合成成骨生长肽在体外可刺激骨髓间充质干细胞增殖和向成骨细胞分化，体内可增加骨密度改善骨质疏松，但具体作用机制尚未明确。
目的：应用小鼠全基因组芯片筛查合成成骨生长肽作用下OPG-/-小鼠骨髓间充质干细胞的差异表达基因，探索成骨生长肽作用下可能影响到的基因与信号传导通路。
方法：应用上海伯豪生物技术有限公司提供的小鼠全基因组Oligo芯片，筛选成骨生长肽干预组与空白对照组OPG-/-小鼠骨髓间充质干细胞差异表达基因，实时定量PCR分析验证部分与增殖分化相关的差异表达基因，并结合聚类分析及通路分析来探索成骨生长肽的作用机制。
结果与结论：芯片结果显示，成骨生长肽作用后使346条基因表达下调，121条基因表达上调。PCR验证结果与芯片结果相符。经BioCarta通路分析，涉及6条通路蛋白；经KEGG通路分析，涉及12条通路。成骨生长肽对OPG-/-小鼠骨髓间充质干细胞的作用涉及多条信号传导通路，其中MAPK信号传导通路可能对其促增殖起着至关重要作用。

关键词: 基因芯片, 骨髓间充质干细胞, 成骨生长肽, 信号传导, 细胞增殖

Abstract:

BACKGROUND: Synthetic osteogenic growth peptide can stimulate proliferation and osteoblast differentiation of bone mesenchymal stem cells (BMSCs) in vitro, and which can enhance bone density in vivo, but the mechanism of the effect of osteogenic growth peptide (OGP) on BMSCs is not clear yet.
OBJECTIVE: To investigate the effect of OGP on gene expression changes in BMSCs using mouse whole-genome microarray and to explore the gene and signal transduction pathway influenced by OGP.
METHODS: Mouse whole-genome Oligo chip was obtained from Shanghai Bo Hao Biotechnology Co., Ltd., gene expression changes in BMSCs of OPG-/- in the OGP group (treated with OGP) and control group were screened. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analyze and verify gene expression changes related to proliferation and differentiation, and which combined with cluster analysis and pathway analysis to explore the mechanism of OPG.
RESULTS AND CONCLUSION: Chip results showed that 346 genes expression down-regulated by OPG, 121 genes expression up-regulated. PCR validation results were consistent with the chip. Six pathways proteins were identified by BioCarta pathway analysis and 12 pathways were identified by KEGG pathway analysis. Multiple pathways were related to the mechanism of the effect of OGP on gene expression changes in BMSCs of OPG-/- mice, among which the MAPK signal transduction pathway may play an important role in their proliferation.

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