中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4941-4945.doi: 10.3969/j.issn.1673-8225.2011.27.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

乙醇诱导大鼠骨髓间充质干细胞的凋亡机制

马爱国，赵劲民，苏  伟，李晓峰，范  锲，崔向荣   

  1. 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市　530021
  • 收稿日期:2011-01-15 修回日期:2011-02-25 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 赵劲民，博士，教授，广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市　530021 zhaojinmin@126.com
  • 作者简介:马爱国★，男，1985年生，广西壮族自治区贵港市人，汉族，广西医科大学在读硕士，医师，主要从事组织工程基础研究。 mag2722@163.com
  • 基金资助:

    广西医疗卫生重点科研课题项目(重200636)；广西研究生教育创新计划项目(2010105981002M188)。

Apoptotic mechanism of rat bone marrow mesenchymal stem cells induced by ethanol

Ma Ai-guo, Zhao Jin-min, Su Wei, Li Xiao-feng, Fan Qie, Cui Xiang-rong   

  1. Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-01-15 Revised:2011-02-25 Online:2011-07-02 Published:2011-07-02
  • Contact: Zhao Jin-min, Doctor, Professor, Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Ma Ai-guo★, Studying for master’s degree, Physician, Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China mag2722@163.com
  • Supported by:

    the Key Medical Research Project of Guangxi, No. 200636*; Guangxi Postdoctoral Educational Innovation, No.2010105981002M188*

PDF

408

被引次数

2

摘要：

背景：有研究表明乙醇可诱导骨髓间充质干细胞凋亡并引起成骨细胞和破骨细胞数量减少，但乙醇对骨髓间充质干细胞凋亡的影响以及作用机制目前尚不十分清楚。
目的：观察乙醇对大鼠骨髓间充质干细胞凋亡、线粒体功能的影响及bcl-2、Caspase-3表达的变化。
方法：应用全骨髓培养法分离培养SD大鼠骨髓间质干细胞，置于0，100，200，300，400，500，600，700，800，900 mmol/L 乙醇中作用24 h，MTT法进行细胞毒性药物实验；置于0，100，200，300，400，500 mmol/L乙醇中作用6，12，24 h，AnnexinV/ PI双标记法流式细胞仪检测细胞凋亡和线粒体膜电位变化情况；置于0，427 mmol/L 乙醇中作用24 h，RT-PCR法检测与凋亡有关的基因bcl-2 和Caspase-3 mRNA表达水平。
结果与结论：MTT检测结果显示427 mmol/L 是乙醇对大鼠骨髓间充质干细胞生长的半数抑制浓度；AnnexinV/ PI检测结果表明，与0 mmol/L组比较，随作用时间的延长与乙醇浓度的增加，骨髓间充质干细胞凋亡率及线粒体跨膜电位的破坏水平明显升高(P < 0.05)。与0 mmol/L组比较，乙醇作用24 h后427 mmol/L组bcl-2 mRNA表达水平下降，Caspase-3 mRNA表达水平增加(P < 0.05)。说明乙醇能诱导大鼠骨髓间充质干细胞凋亡，凋亡的发生可能与线粒体膜电位破坏、线粒体功能障碍、bcl-2和Caspase-3激活有关。

关键词: 乙醇, 骨髓间充质干细胞, 凋亡, AnnexinV/PI双标记法, 线粒体膜电位, 大鼠, RT-PCR, bcl-2, Caspase-3

Abstract:

BACKGROUND: Previous studies demonstrated that ethanol can induce apoptosis in bone marrow mesenchymal stem cells (BMSCs), and decrease the number of osteoplasts and osteoclasts. However, the effect and mechanism of ethanol on apoptosis in BMSCs remains unclear.
OBJECTIVE: To investigate the effect of ethanol on apoptosis in BMSCs of rats and their mitochondrial function and to evaluate the pathway associated with the regulation of Bcl-2 and Caspase-3.
METHODS: BMSCs were isolated from Sprague-Dawley rats were treated with ethanol at doses of 0, 100, 200, 300, 400, 500, 600, 700, 800, 900 mmol/L for 24 hours. cytotoxic drug experiment was performed with MTT assay. BMSCs were treated with ethanol at doses of 0, 100, 200, 300, 400, 500 mmol/L for 6, 12, 24 hours, AnnexinV/PI flow cytometry of double label method was performed to detect the apoptosis and mitochondrial membrane potential, BMSCs were treated with ethanol at doses of 0, 427 mmol/L for 24 hours, the levels of Bcl-2 and Caspase-3 mRNA expression were determined by RT-PCR method.
RESULTS AND CONCLUSION: MTT assay results showed that 50% concentration of inhibition (IC50) of BMSCs of rats grew in ethanol was 427 mmol/L. The results of Annexin V/PI assay indicated that the apoptosis rates of BMSCs and mitochondrial membrane potential were higher than that of untreated (0 mmol/L) group when time and dose of ethanol was increased (P < 0.05). Compared with 0 mmol/L group, the level of Bcl-2mRNA expression was decreased in 427 mmol/L group after 24 hours, but the caspase-3 mRNA expression was increased significantly by treatment at 427 mmol/L (P < 0.05). These results suggest that ethanol can induce apoptosis in BMSCs of SD rat, and the occurrence of apoptosis may be related to the mitochondrial membrane potential damage, mitochondrial dysfunction, bcl-2 and Caspase-3 activation.

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