中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4387-4390.doi: 10.3969/j.issn.1673-8225.2011.24.005

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

外固定方法制作的大鼠股骨骨折模型

赵震宇1，邵　林1，刘建宇1，杨大平2，郝晨光1，赵洪梅3，刘国锋2   

  1. 哈尔滨医科大学附属第二医院，1骨外科，2整形外科，黑龙江省哈尔滨市 150001；3哈尔滨医科大学附属第三医院妇科，黑龙江省哈尔滨市 150040
  • 收稿日期:2010-12-19 修回日期:2011-02-20 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 邵林，教授，主任医师。哈尔滨医科大学附属第二医院骨外科，黑龙江省哈尔滨市 150001 linshao58@yeah.net
  • 作者简介:赵震宇☆，男，1978年生，博士，主治医师，主要从事骨组织工程方面的研究。 zhenyuzhao78@yeah.net
  • 基金资助:

    黑龙江省教育厅科研项目(11511171)。

Preparation of rat femoral fracture model with an external fixator

Zhao Zhen-yu1, Shao Lin1, Liu Jian-yu1, Yang Da-ping2, Hao Chen-guang1, Zhao Hong-mei3, Liu Guo-feng2   

  1. 1Department of Bone Surgery, 2Department of Plastic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China; 3Department of Gynecology, Third Affiliated Hospital of Harbin Medical University, Harbin  150040, Heilongjiang Province, China
  • Received:2010-12-19 Revised:2011-02-20 Online:2011-06-11 Published:2011-06-11
  • Contact: Shao Lin, Professor, Chief physician, Department of Bone Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China linshao58@yeah.net
  • About author:Zhao Zhen-yu☆, Doctor, Attending physician, Department of Bone Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China zhenyuzhao78@ yeah.net
  • Supported by:

    the Science and Technology Program of Education Department of Heilongjiang Province, No 11511171*

PDF

581

被引次数

19

摘要：

背景：外固定器抗旋转能力强，不影响局部血运，不固定关节，动物可以自由活动，适合用于骨折模型的制作。
目的：创立一种新型的股骨骨折动物模型。
方法：SD大鼠24只于大鼠右侧股骨截骨，以自行研究设计的微型单边外固定器为固定装置，制成骨折模型，术后通过大体观察、影像学观察及组织学方法观察股骨对位情况和截骨端的愈合情况。
结果与结论：术后外固定器保持正常位置，未见螺纹钉拔出，针孔松动，骨折等现象，大鼠股骨保持正常生理位置。固定后第2周骨断端已经有模糊的原始骨痂形成。第4周时已有连续性骨痂通过，但骨断端仍较清晰。第8周时，骨断端已经愈合，髓腔再通，出现骨性愈合。证实微型单边外固定器可以为股骨骨折模型大鼠提供可靠的固定，并且右侧股骨截骨骨折模型大鼠制作合理。

关键词: 骨折模型, 外固定器, 大鼠, 组织工程, 骨折愈合

Abstract:

BACKGROUND: The external fixator has strong anti-rotation capability, thus, it not affect local blood supply or binding joints, which is suitable for preparing bone fracture models. 
OBJECTIVE: To design a new type animal model to study the fracture.
METHODS: Twenty-four right SD rats were performed right femoral osteotomy and fixed by a newly developed external fixator. Gross observation, imaging observation and histological method were used to observe the femoral positioning and healing of osteotomized bone ends.
RESULTS AND CONCLUSION: The external fixator maintained in the correct location of femur, with no signs of screw pull out, pinhole loosen, or fracture. Callus formation could be vague seen at 2 weeks after fixation. There were successive callus at 4 weeks, but the fracture end still clear. At 8 weeks after fixation, bone union could be found. The results demonstrated that external fixator utilized has sufficient stability for the management of fracture. This is a successful model of fracture in rats.

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