中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4340-4343.doi: 10.3969/j.issn.1673-8225.2011.23.037

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

脂肪组织来源种子细胞的获取和分化潜能

朱晓斐1，王  飏2，贾立辉3   

  1. 1辽宁医学院研究生院，辽宁省锦州市 121000；解放军沈阳军区总医院，2整形科，3科训科，辽宁省沈阳市  110016
  • 收稿日期:2011-03-01 修回日期:2011-04-06 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 贾立辉，硕士生导师，副主任，科训科科长，解放军沈阳军区总医院科训科，辽宁省沈阳市 110016
  • 作者简介:朱晓斐★，女，汉族，山东省烟台市人，辽宁医学院在读硕士，主要从事口腔临床医学颌面外科研究。 xiaofei.987@163.com

Harvesting and differentiation potential of seed cells from adipose tissue

Zhu Xiao-fei1, Wang Yang2, Jia Li-hui3   

  1. 1Graduate Department, Liaoning Medical University, Jinzhou  121000, Liaoning Province, China; 2Department of Plastic Surgery, 3Department of Training, General Hospital of Shenyang Military Command, Shenyang  110016, Liaoning Province, China
  • Received:2011-03-01 Revised:2011-04-06 Online:2011-06-04 Published:2011-06-04
  • Contact: Jia Li-hui, Master’s supervisor, Department of Training, General Hospital of Shenyang Military Command, Shenyang 110016, Liaoning Province, China
  • About author:Zhu Xiao-fei★, Studying for master’s degree, Graduate Department, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China xiaofei.987@163.com

PDF

259

被引次数

4

摘要：

背景：组织工程中种子细胞以骨髓干细胞居多，但随着其来源有限，提取创伤大等一系列问题的限制，研究人员开始寻找其他的种子细胞。脂肪组织以其丰富的来源、提取方便等优势逐渐得到重视。以脂肪组织为来源的种子细胞可能将具有广阔的应用前景。
目的：综述脂肪组织来源的种子细胞的研究进展。
方法：由第一作者应用计算机检索2000/2010 PubMed数据库及重庆维普数据库有关脂肪干细胞获取和分化能力以及去分化脂肪细胞、诱导多能干细胞等在相关组织工程中应用前景方面的文献。     
结果与结论：脂肪组织因其丰富的来源，便捷的取材方法和较高的干细胞含量使其相比骨髓更适合作为组织工程中种子细胞的供体。目前研究较多的是脂肪干细胞，近年来随着“去分化”和基因转导技术的日益发展，去分化脂肪细胞和多潜能诱导干细胞的获取和生物学性能研究也有了较大的进展。作为种子细胞，3种细胞有着各自的优势和缺陷，但均可来源于脂肪组织，对其各自的获取方法和诱导分化潜能的研究比对，有助于人们在组织工程应用中对种子细胞的选择。  

关键词: 种子细胞, 脂肪干细胞, 去分化脂肪细胞, 诱导性多能干细胞, 干细胞

Abstract:

BACKGROUND: Bone marrow stromal cells (BMSCs) are considered as one kind of important seed cells in tissue engineering. At the same time BMSCs finite resource and pain while obtaining from patients has limited the application of BMSCs in tissue engineering. Adipose tissue with the plenty resource and easy to obtain has been paid more attention. Maybe seed cells derived from adipose tissue can be used generally in the future.
OBJECTIVE: To review the research on application of different kinds of seed cells derived from adipose tissue in tissue engineering.
METHODS: The first author searched articles about the isolation and differentiation potential of adipose derived stem cells, dedifferentiated adipocytes and induced pluripotent stem cells in PubMed and CQVip (2000/2010) databases by computer. 
RESULTS AND CONCLUSION: Cells derived from adipose tissue are more suitable to be seed cells for tissue engineering than BMSCs, for their availability, higher purity, and abundance in body. Nowadays, research on adipose derived stem cells is a hot spot. With the development of dedifferentiation and gene-transduction techniques, both the studies on biological characteristics and application of dedifferentiated adipocytes and induced pluripotent stem cells have made great progress. As seed cells, all three kinds of cells mentioned above can be obtained from adipose tissue, but with individual advantage and deficiency. Emphasis is placed on the contrast of acquisition and pluripotency among them, through which we can choose the ideal seed cells for tissue engineering.

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