中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4229-4232.doi: 10.3969/j.issn.1673-8225.2011.23.012

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

维生素C体外诱导多能干细胞向心肌细胞的分化

杨  梁，张晓刚，魏新伟   

  1. 重庆医科大学附属第一医院心内科，重庆市400016
  • 收稿日期:2010-12-06 修回日期:2011-04-22 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 张晓刚，博士，教授，硕士生导师，重庆医科大学附属第一医院心内科，重庆市 400016 zxg0233@sina.com
  • 作者简介:杨梁★，男，1985年生，汉族，四川省成都市人，重庆医科大学在读硕士，医师，主要从事诱导多能干细胞向心肌细胞分化方面的研究。 yangliang428@163.com
  • 基金资助:

    重庆市卫生局医学科学技术研究重点项目(20090151)，课题名称“细胞或组织心脏移植的电生理研究”。

Vitamin C promotes differentiation of mouse pluripotent stem cells into cardiomyocytes in vitro

Yang Liang, Zhang Xiao-gang, Wei Xin-wei   

  1. Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2010-12-06 Revised:2011-04-22 Online:2011-06-04 Published:2011-06-04
  • Contact: Zhang Xiao-gang, Doctor, Professor, Master’s supervisor, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China zxg0233@sina.com
  • About author:Yang Liang★, Studying for master’s degree, Physician, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China yangliang428@163.com
  • Supported by:

    the Science and Technology Research Major Program of Chongqing Health Bureau, No. 20090151*

PDF

468

被引次数

6

摘要：

背景：诱导多能干细胞治疗缺血性心脏病被认为是极具前景的方法，但目前仍未找到一种理想的诱导方式。
目的：观察维生素C作为诱导剂对多能干细胞体外分化为心肌细胞的影响。
方法：复苏小鼠诱导多能干细胞，传代培养后，采用直接悬浮培养法使小鼠诱导多能干细胞形成拟胚体，用含10-3，10-4，10-5，10-6 mol/L 4种不同浓度维生素C的分化培养基对其进行诱导分化，以不添加任何诱导剂作为对照组，观察各组出现搏动拟胚体的数量，计算分化比率。
结果与结论：维生素C诱导小鼠诱导多能干细胞分化为心肌细胞的最佳浓度为10-4 mol/L，有(57.00±3.20)%的拟胚体出现搏动，显著高于不添加任何诱导剂的对照组(5.13%±0.55)%(P < 0.01)。诱导而来的心肌细胞表达心肌特异性蛋白cTnT以及α-actin。提示最佳的维生素C诱导分化条件能够促进小鼠诱导多能干细胞向心肌细胞分化，提高其分化效率。

关键词: 诱导多能干细胞, 拟胚体, 维生素C, 分化, 心肌细胞

Abstract:

BACKGROUND: Inducing pluripotent stem cells have been considered promising treatment for ischemic heart disease. However, an ideal inducing method has not been found yet.
OBJECTIVE: To investigate the effect of vitamin C on the cardiomyocytes differentiation from mouse induced pluriptent stem cells (miPS).
METHODS: miPS were recovered, passaged and form embryoid bodies. The embryoid bodies were induced by differentiation medium containing various concentration of vitamin C (10-3, 10-4, 10-5, 10-6 mol/L). Control group was not treated by inducer. The number of beating embryoid bodies were calculated.
RESULTS AND CONCLUSION: The best concentration of vitamin C to promote the cardiac differentiation of miPS was
10-4 mol/L. Beating area was found in 57.00%±3.20% of embryoid bodies, significantly greater than control group (5.13%±0.55%; P < 0.01). Special cardiac proteins α-actin and cTnT were detected. Results showed that vitamin C could redound differentiation of miPS into cardiomyocytes.

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