中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (22): 4111-4114.doi: 10.3969/j.issn.1673-8225.2011.22.029

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

核因子κB、基质金属蛋白酶3在退变腰椎间盘组织中的表达

原  野1，赵  静2，李永民1，王  旭1，谷守山1   

  1. 1唐山市第二医院脊柱外科，河北省唐山市 063000
    2河北联合大学病理教研室，河北省唐山市  063000
  • 收稿日期:2011-01-09 修回日期:2011-03-24 出版日期:2011-05-28 发布日期:2011-05-28
  • 通讯作者: 赵静，副教授，河北联合大学病理教研室，河北省唐山市 063000 zhaojingts@163.com
  • 作者简介:原野★，男，1971年生，黑龙江省鸡西市人，汉族，1996年华北煤炭医学院毕业，硕士，副主任医师，主要从事椎间盘退变的分子病理学研究。 yuanyets@163.com
  • 基金资助:

    河北省教育厅自然科学指导计划项目(Z2005102)，课题名称“TGF-1、MMPs-1、MMPs-3在退变椎间盘组织中的表达及相关性研究”。

Expression of nuclear factor kappa B and matrix metalloproteinases 3 in intervertebral disc degeneration

Yuan Ye1, Zhao Jing2, Li Yong-min1, Wang Xu1, Gu Shou-shan1   

  1. 1Department of Spinal Surgery, the Second Hospital of Tangshan, Tangshan  063000, Hebei Province, China
    2Department of Pathology, Hebei United University, Tangshan  063000, Hebei Province, China
  • Received:2011-01-09 Revised:2011-03-24 Online:2011-05-28 Published:2011-05-28
  • Contact: Zhao Jing, Associate professor, Department of Pathology, Hebei United University, Tangshan 063000, Hebei Province, China zhaojingts@163.com
  • About author:Yuan Ye★, Master, Associate chief physician, Department of Spinal Surgery, the Second Hospital of Tangshan, Tangshan 063000, Hebei Province, China yuanyets@163.com
  • Supported by:

    the Natural Science Instruction Plan of Hebei Education Bureau, No. Z2005102*

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摘要：

背景：对于椎间盘退变的原因一直是国内外学者研究的重点。近年来随着研究的深入，在椎间盘退变过程中多种细胞因子、黏附分子、趋化因子、炎症递质、蛋白质酶等发挥着重要作用。
目的：比较正常腰椎间盘组织及退变腰椎间盘组织中核因子κB、基质金属蛋白酶3的表达，分析两者的相关性。
方法：收集唐山市第二医院脊柱外科手术切除退变椎间盘组织68例(病变组)，腰椎爆裂骨折正常椎间盘组织10例(对照组)，应用苏木精-伊红染色、免疫组织化学方法和ELISA方法检测核因子κB、基质金属蛋白酶3的表达，并对两者相关性进行分析。
结果与结论：苏木精-伊红染色结果显示对照组可见纤维环和髓核结构，腰椎间盘髓核的软骨细胞表现为不规则的软骨陷窝，病变组细胞增殖明显，细胞核呈圆形或卵圆形，髓核细胞胞质呈空泡状。免疫组织化学方法显示病变组核因子κB、基质金属蛋白酶3吸光度值明显高于对照组(P < 0.05)。ELISA检测病变组核因子κB、基质金属蛋白酶3的表达均高于对照组(P < 0.05)。两者具有正相关性(r=0.643 0，P < 0.01)。

关键词: 腰椎间盘, 退变, 免疫组织化学, ELISA核因子&kappa, B, 基质金属蛋白酶3

Abstract:

BACKGROUND: During intervertebral disc degeneration, various cytokines, adhesion molecules, chemokines, inflammatory neurotransmitters, protein enzymes play an important role.
OBJECTIVE: To study the expression and significance of nuclear factor kappa B (NF-κB) and matrix metalloproteinases 3 (MMP-3) in normal disc tissue and intervertebral disc degeneration.
METHODS: Sixty-eight patients with intervertebral disc degeneration from surgical intervention for cervical disc herniation (lesion group) and 10 cases of normal disc tissue (control group) were included. The expressions of NF-κB and MMP-3 were tested by hematoxylin-eosin staining, immunohistochemistry method and ELISA.
RESULTS AND CONCLUSION: The NF-κB and MMP-3 positive expression rate was higher in the control group (P < 0.05). There was a positive correlation (r=0.643 0, P< 0.01). It indicated that NF-κB and MMP-3 may have important effects on intervertebral disc degeneration.

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