中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3715-3718.doi: 10.3969/j.issn.1673-8225.2011.20.025

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

马钱子碱诱导人多发性骨髓瘤细胞株U266细胞凋亡与bax基因的表达

李志华，马艳萍，王艺华，冯  静，李凌飞   

  1. 山西医科大学第二医院血液科，山西省太原市  030001
  • 收稿日期:2010-11-19 修回日期:2011-01-09 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 马艳萍，博士，主任医师，山西医科大学第二医院血液科，山西省太原市 030001 yanpingma@ hotmail.com
  • 作者简介:李志华★，女, 1983年生，山西省定襄县人，汉族，山西医科大学在读硕士，主要从事多发性骨髓瘤基础与临床研究。 lizhihuasx@163.com
  • 基金资助:

    山西省留学人员管理委员会办公室资助项目，编号：(2008)10号-99，课题名称：多发性骨髓瘤耐药分子特性研究。

Effects of brucine on apoptosis of multiple myeloma cell line U266 and bax gene expression

Li Zhi-hua, Ma Yan-ping, Wang Yi-hua, Feng Jing, Li Ling-fei   

  1. Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Received:2010-11-19 Revised:2011-01-09 Online:2011-05-14 Published:2011-05-14
  • Contact: Ma Yan-ping, Doctor, Chief physician, Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China yanpingma@hotmail.com
  • About author:Li Zhi-hua★, Studying for master’s degree, Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China lizhihuasx@163.com
  • Supported by:

    the Item of Shanxi Scholarship Council of China, No. (2008)10-99*

PDF

423

摘要：

背景：马钱子碱具有抗肿瘤作用，而其对人多发性骨髓瘤细胞生长的影响尚不明确。
目的：探讨马钱子碱诱导人多发性骨髓瘤U266细胞凋亡的机制。
方法：取对数生长期的U266细胞，分别用0，0.05，0.1，0.2，0.4 g/L的马钱子碱干预12，24，48 h，通过MTT法测得IC50，并以此浓度干预U266细胞，通过形态学观察、RT-PCR法检测马钱子碱对人多发性骨髓瘤U266细胞株的诱导凋亡作用。
结果与结论：马钱子碱诱导U266的凋亡呈时间和浓度依赖性(P < 0.05)，其作用48 h的IC50为0.16 g/L。在马钱子碱诱导的凋亡细胞中可见凋亡小体，同时，细胞中的促凋亡基因bax的表达量随马钱子碱作用时间的延长逐渐增加(P < 0.01)。说明0.4 g/L浓度范围内的马钱子碱具有诱导U266细胞凋亡的作用，这种作用具有浓度和时间依赖性，可能是通过激活bax基因途径实现的。

关键词: 马钱子碱, 细胞凋亡, 细胞周期, bax基因, U266细胞

Abstract:

BACKGROUND: Brucine has anti-tumor effect. However, its influence on growth of multiple myeloma cells is not clear.
OBJECTIVE: To investigate apoptotic mechanism of brucine on human multiple myeloma.
METHODS: Logarithmic growth phase of U266 cells were treated with 0, 0.05, 0.1, 0.2, and 0.4 g/L brucine for 12, 24, and 48 hours, measured IC50 by MTT, and interfere the U266 cells. The apoptotic effect of brucine on human multiple myeloma cell line-U266 was observed by morphological observation and RT-PCR.
RESULTS AND CONCLUSION: Brucine induced U266 apoptosis showed a time- and concentration-dependent manner (P < 0.05). The IC50 level was 0.16 g/L at 48 hours after intervention. Apoptotic body could be found in brucine treated-apoptotic cells, meantime, the expression of pro-apoptosis gene bax was gradually increased with time prolonged (P < 0.01). It is suggest that 0.4 g/L concentration brucine induced U266 apoptosis, and the effect showed in a concentration- and time-dependent manner, which possibly through the activation of bax pathway.

中图分类号: