中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3542-3545.doi: 10.3969/j.issn.1673-8225.2011.19.029

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

三维重构展示类胚体样结构

沈晓涛1，2，3，郑  馨1，2，3，钟嘉泳4，张志鹏4，李  震1，2，3，蔡冬青1，2，3   

  1. 暨南大学，1再生医学教育部重点实验室，2再生医学香港中文大学-暨南大学联合实验室，3科技部及广东省科技厅国际科技合作基地，4生物医学工程系，广东省广州市  510632
  • 收稿日期:2011-01-19 修回日期:2011-03-17 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 蔡冬青，教授，博士生导师，暨南大学再生医学教育部重点实验室，广东省广州市 510632 tdongbme@jnu.edu.cn
  • 作者简介:沈晓涛★，女，1980年生，广东省湛江市人，汉族，2009年暨南大学毕业，硕士，助理实验师，暨南大学再生医学教育部重点实验室，广东省广州市 510632 tsxt@jnu.edu.cn
  • 基金资助:

    国家“863”资助项目(2007AA02Z105)；国家自然科学基金资助项目(30973158，30770886，30570369，30340038)；广东省自然科学基金重点项目(04105826)；广东省科技攻关项目(2004B30601007)；广东省国际合作项目(B05090007)；中央高校基本科研业务费专项资金资助(21609408)。

Three-dimensional reconstruction and display of embryoid body-like structure

Shen Xiao-tao1,2,3, Zheng Xin1,2,3, Zhong Jia-yong4, Zhang Zhi-peng4, Li Zhen1,2,3, Cai Dong-qing1,2,3   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-19 Revised:2011-03-17 Online:2011-05-07 Published:2011-05-07
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Shen Xiao-tao★, Master, Assistant technician, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China tsxt@jnu.edu.cn
  • Supported by:

    the National “863” Program, No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30973158*, 30770886*, 30570369*, 30340038*; Guangdong Natural Science Foundation, No. 04105826*; Guangdong Science and Technology Development Program, No. 2004B30601007*; International Collaborated Program of Guangdong, No.  2009B050900007*; The Fundamental Research Funds for the Central Universities, No. 21609408*

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摘要：

背景：干细胞因其具有增殖能力及分化潜能，被认为是实施细胞治疗的重要细胞而被广泛研究。实现干细胞与组织三维结构的展示与分析，对阐明干细胞增殖与分化的机制有重要意义。
目的：探讨实现对经分化的类胚体样组织结构进行三维重构展示的方法。
方法：以来源于骨骼肌干细胞经诱导具有三维立体结构的类胚体作为实验对象，利用多色免疫荧光染色结合Z轴连续图像采集和三维重构技术，对经分化的类胚体样组织结构进行三维重构展示。
结果与结论：利用实验方法可实现对类胚体样组织结构进行三维重构，经重构的三维模型可实现任意角度旋转，利用不同荧光通道iso-surface处理后，选择分别显示不同颜色功能或任意横断面展示功能，即可展示心脏特异性肌钙蛋白Ⅰ阳性组织结构与细胞核的空间位置关系，并清晰展示在经分化形成类心肌组织的结构中存在细胞核。提示利用该技术，可对经分化的类胚体样组织结构进行三维重构展示及测量。

关键词: 类胚体样结构, 三维重构, Z轴扫描, 三维测量, 干细胞

Abstract:

BACKGROUND: Stem cells have been widely studied and are considered as important cells for cell therapy since stem cells are capable of proliferation and differentiation. It is believed that three-dimensional (3D) reconstruction and display of stem cells and tissues is important to better understand the mechanisms of proliferation and differentiation of stem cells.
OBJECTIVE: To reveal and analyze embryoid body-like tissue structure using 3D-reconstruction technique.
METHODS: Differentiated embryoid body-like tissue structure was used as a model. Immunofluorescent staining combined with Z-axis consecutive image capture and 3D-reconstruction were used to reveal and analyze differentiated embryoid body-like cardiac myocyte tissue.
RESULTS AND CONCLUSION: Methods used in this study were able to reveal 3D-reconstruction of embryoid body-like tissue structure. Reconstructed 3D model could be rotated at any angle. After iso-surface processing under certain color channel, spatial relationship of cardiac troponin I positive tissue structure and nucleus could be displayed on related color channel or at any angle dimension. It was found that the nuclei were included in the differentiated embryoid body-like cardiac myocyte tissue. The results of present study documented that differentiated embryoid body-like cardiac myocyte tissue was able to reveal and analyze using the 3D-reconstruction technique.

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