中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3443-3446.doi: 10.3969/j.issn.1673-8225.2011.19.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓源干细胞参与的血管内皮更新

许翼麟，何向辉，魏  伟，章志翔，朱理玮   

  1. 天津医科大学总医院普通外科研究所，天津市300052
  • 收稿日期:2010-11-15 修回日期:2010-12-10 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 何向辉，博士，教授，天津医科大学总医院普通外科研究所，天津市300052 humphreyhe@163.com
  • 作者简介:许翼麟☆，男，1981年生，伯利兹籍华人，汉族，天津医科大学总医院普通外科研究所在读博士，主要从事腹部肿瘤及免疫基因治疗方面的研究。 evanhsu@hotmail.com
  • 基金资助:

    国家自然科学基金资助项目(30870967) ，课题名称“骨髓源干细胞融合促进恶性肿瘤发生和进展的实验研究”。

Bone marrow-derived stem cells involved in the renewal of vascular endothelium

Xu Yi-ling, He Xiang-hui, Wei Wei, Zhang Zhi-xiang, Zhu Li-wei   

  1. Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin  300052, China
  • Received:2010-11-15 Revised:2010-12-10 Online:2011-05-07 Published:2011-05-07
  • Contact: He Xiang-hui, Doctor, Professor, Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin 300052, China humphreyhe@163.com
  • About author:Xu Yi-ling☆, Studying for doctorate, Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin 300052, China evanhsu@hotmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30870967*

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摘要：

背景：研究表明骨髓源干细胞可塑性强，但其参与组织更新及修复的机制在转分化及细胞融合间尚存在争议。
目的：通过性别交叉骨髓移植建立雌雄嵌合体小鼠模型，观察骨髓源性干细胞是否参与内皮细胞的更新并探讨其可能的机制。
方法：采用雌性C57BL/6-GFP小鼠为供体，雄性C57BL/6小鼠为受体进行骨髓移植建立嵌合体小鼠，并应用流式细胞术分析骨髓嵌合情况。骨髓移植后20周采用Y染色体荧光原位杂交法对脑、肾、肝、脾及心脏组织Y染色体进行标记，荧光显微镜观察各组织器官中血管内皮细胞Y染色体及绿荧光蛋白表达情况。
结果与结论：①嵌合体小鼠骨髓细胞流式细胞学检测显示移植骨髓后1周骨髓GFP+细胞为(7.48±1.38)%、4周时达(73.92±5.57)%，结果表明成功建立性别交叉骨髓移植模型。②骨髓移植后20周嵌合体小鼠脑、肾、肝和脾组织血管壁可见绿荧光蛋白表达，且部分细胞同时表达Y染色体，表明发生骨髓源细胞和血管内皮细胞融合。脑实质及心肌组织未见绿荧光蛋白表达。结果提示骨髓源干细胞可能通过细胞融合机制参与不同组织器官中血管内皮细胞的更新。

关键词: 骨髓移植, 干细胞, 转基因小鼠, 内皮细胞, 细胞融合

Abstract:

BACKGROUND: Bone marrow-derived stem cells have strong plasticity, but the mechanism that they participate in regeneration and renewal of tissue is still conflicting between transdifferentiation and cell fusion.
OBJECTIVE: To investigate the possibility and possible mechanism of bone marrow-derived stem cells involvement in the renewal of vascular endothelial cells in different organs through sex crossing bone marrow transplantation using mouse models of sexual mosaic.
METHODS: Female C57BL/6-green fluorescent protein (GFP) mice were used as donor mice, and male C57BL/6 mice were used as the recipient to establish bone marrow transplanted chimeric mice. The chimerism of bone marrow was assessed by flow cytometry. Twenty weeks after transplantation, frozen slide of the brain, kidney, liver, spleen, and heart were prepared and fluorescence in situ hybridization was applied to label the Y chromosome, then GFP expression and Y chromosome were observed using fluorescence microscope.
RESULTS AND CONCLUSION: The percent of GFP+ cell of bone marrow cells in the recipient was (7.48±1.38)% and (73.92±5.57)% at week 1 and week 4 respectively, indicating the success of sex crossing bone marrow transplantation model. In week 20, GFP expression was detected in vascular endothelial cells in the brain, kidney, liver, and spleen, and some cells also showed Y chromosome expression, indicating cell fusion occurred. No GFP expression was observed within the parenchyma of the brain and heart. Results have indicated that bone marrow-derived stem cells might have participated in the renewal of vascular endothelial cells in different organs through cell fusion.

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