中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3347-3350.doi: 10.3969/j.issn.1673-8225.2011.18.028

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

大鼠原位肾脏移植模型的建立

麦海星，曲  楠，赵  立，黄  晨，王亚林，李学超，李建涛，陈立军   

  1. 解放军军事医学科学院附属医院泌尿外科，北京市   100071
  • 收稿日期:2010-10-26 修回日期:2010-12-02 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 陈立军，博士，教授，硕士生导师，解放军军事医学科学院附属医院泌尿外科，北京市 100071 chenlijun829@hotmail.com
  • 作者简介:麦海星★，男，1981年生，广东省阳江市人，汉族，2008年解放军第四军医大学毕业，硕士，主要从事肾脏移植的研究。 maimark24@hotmail.com

Rat models of orthotopic kidney transplantation

Mai Hai-xing, Qu Nan, Zhao Li, Huan Chen, Wang Ya-lin, Li Xue-chao, Li Jian-tao, Chen Li-jun   

  1. Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China
  • Received:2010-10-26 Revised:2010-12-02 Online:2011-04-30 Published:2011-04-30
  • Contact: Chen Li-jun, Doctor, Professor, Master’s supervisor, Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China chenlijun829@hotmail.com
  • About author:Mai Hai-xing★, Master, Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China maimark24@hotmail.com

PDF

508

被引次数

7

摘要：

背景：目前已有多种大鼠肾移植模型建模方式,但在移植时间、移植效果等方面都存在各种问题。
目的：探讨建立稳定、可靠的大鼠原位肾脏移植模型的方法。
方法：以SD大鼠为供体Wistar大鼠为受体行原位肾移植术。供体肾动脉、肾静脉在自制橡胶垫片上分别与受体的肾动脉、肾静脉端端吻合，供体输尿管膀胱瓣与受体膀胱吻合。将实验动物随机分为2组，对照组移植后每日腹腔内输注1 mL D-hanks液；环孢素A组移植后每日皮下注射环孢素A 15 mg/kg。记录大鼠生存时间并于移植后第3，5，10天测定血肌酐值，移植后第10天，光镜下观察移植肾病理改变。
结果与结论：大鼠原位肾脏移植成功率为85%。移植后第5，10天环孢素A组血清肌酐值显著低于对照组 (P < 0.05)。环孢素A组大鼠肾移植后存活天数明显长于对照组(P < 0.05)，移植肾病理可见排斥明显减轻。提示该模型稳定性强、重复性好，具有较高的成功率。

关键词: 模型, 动物, 肾, 移植, 显微外科, 大鼠

Abstract:

BACKGROUND: There are many ways to prepare kidney transplantation models in rats; however, there are still many problems about operation time, transplantation effect and so on.
OBJECTIVE: To study the microsurgical technique of establishing a reliable rat model of orthotopic kidney transplantation.
METHODS: Kidney transplantation was performed from SD to Wistar strain (allogeneic),the donor’s artery and renal vein were put on the self-make rubber septum and underwent the end to end anastomosis surgeon with the receptor’s arteriae renalis and renal vein. After that, the donor’s bladder valva was inosculated with the receptor’s bladder. All the rats were divided into two groups: control group and Cyclosporin A (CsA) group, each group included 30 rats. The control group received 1 mL D-hanks each day after transplantation; the CsA group received subcutaneous injection of CsA for 15 mg/kg. The serum creatinine levels were observed at days 3, 5 and 10 after transplantation, pathological changes were also observed at 10 days after transplantation.  
RESULTS AND CONCLUSION: Rat orthotopic kidney transplantation was performed in 60 rats, and the successful rate was 85%. The serum creatinine level in the CsA group was lower than that in the control group (P < 0.05), but the survival time in the CsA group was longer than that in the control group (P < 0.05). Allografts of the control group exhibited typical severe acute rejection. It is indicated that this rat model of kidney transplantation is a reliable model with good reproducibility and high achievement ratio.

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