中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6374-6376.doi: 10.3969/j.issn.1673-8225.2010.34.025

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

生物素化聚乙二醇/聚乳酸纳米粒子的体外主动靶向行为

龚妍春，熊向源，李资玲，李玉萍，郭  亮   

  1. 江西科技师范学院生命科学学院，江西省南昌市  330013
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 熊向源，博士，教授，江西科技师范学院生命科学学院，江西省南昌市 330013 xyxiong@gmail.com
  • 作者简介:龚妍春★，女，1982年生，江西省南昌市人，汉族，2007年南昌大学医学院毕业，硕士，助教，主要从事新型高分子药物制剂的研究。 spoilming@163.com
  • 基金资助:

    国家自然科学基金项目(50763003)；教育部留学回国人员科研启动基金(教外司留[2007]1108号)；人事部留学人员科技活动项目择优资助经费(国人厅发[2006]164号)；江西省自然(青年)科学基金项目(2007GQC1094)；江西科技师范学院自然科学课题(KY2008ZY09)。

Active targeting behaviors of biotinylated poly(ethylene oxide)/poly(lactic acid) nanoparticles in vitro

Gong Yan-chun, Xiong Xiang-yuan, Li Zi-ling, Li Yu-ping, Guo Liang   

  1. School of Life Science, Jiangxi Science & Technology Normal University, Nanchang   330013, Jiangxi Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Xiong Xiang-yuan, Doctor, Professor, School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi Province, China xyxiong@gmail.com
  • About author:Gong Yan-chun★, Master, Teaching assistant, School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi Province, China spoilming@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50763003*; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, No.[2007]1108*; the Preferred Scientific Research Foundation of Studying Abroad by the State Human Resource Ministry, No. [2006]164*; Natural (Young) Science Foundation of Jiangxi Province, No. 2007GQC1094*; Natural Science Foundation of Jiangxi Science & Technology Normal University, No. KY2008ZY09*

PDF

523

被引次数

12

摘要：

背景：目前研究的大部分高分子药物载体没有靶向性，在应用上有局限性，只有几个国外课题组报道生物素化聚乙二醇/聚乳酸(Biotin-PEO-PLA)纳米粒子的体外靶向行为，国内没有这方面的研究报道。
目的：分析Biotin-PEO-PLA纳米粒子作为靶向药物载体的可行性。
方法：透析法制备包埋紫杉醇的Biotin-PEO-PLA纳米粒子并表征；通过高效液相色谱研究包埋紫杉醇的Biotin-PEO-PLA纳米粒子的体外释放行为；利用细胞毒性法比较研究生物素-亲和素三步法实施的包埋紫杉醇的Biotin-PEO-PLA纳米粒子对OVCAR-3(表面表达CA-125抗体)和SKOV-3(表面不表达CA-125抗体)细胞的体外靶向行为。
结果与结论：包埋在Biotin-PEO-PLA纳米粒子中的紫杉醇的释放呈现初期的快速释放以及随后的缓慢释放。利用三步法处理的OVCAR-3细胞存活率明显低于SKOV-3细胞，表明通过Biotin-PEO-PLA/avidin/biotinylated MAB X306与OVCAR-3细胞表面CA-125抗原的特异性相互作用，包埋紫杉醇的Biotin-PEO-PLA纳米粒子被更为有效地传递进了OVCAR-3细胞。

关键词: 嵌段共聚物, Biotin-PEO-PLA, 纳米粒子, 靶向药物输送, 紫杉醇

Abstract:

BACKGROUND: Recently, the majority of polymer drug carriers have no targeting, thus are limited in applications. A few foreign research groups reported in vitro targeting behaviors of biotinylated poly(ethylene oxide)-poly(lactic acid) (Biotin-PEO-PLA) nanoparticles, however, the domestic study is absent.
OBJECTIVE: To verify the feasibility of Biotin-PEO-PLA nanoparticles as targeted drug carriers.
METHODS: Paclitaxel-loaded Biotin-PEO-PLA nanoparticles were prepared by dialysis method and characterized. The in vitro release behaviors of paclitaxel-loaded Biotin-PEO-PLA nanoparticles were studied by high performance liquid chromatogram technique. The active targeting properties of paclitaxel-loaded Biotin-PEO-PLA nanoparticles in vitro over OVCAR-3 (CA-125 antigen positive) and SKOV-3 cells (CA-125 antigen negative) were also investigated through a three-step biotin-avidin interaction by MTT tests.
RESULTS AND CONCLUSION: Paclitaxel loaded in Biotin-PEO-PLA nanoparticles shows an initial rapid release followed by a slow release period. Compared with SKOV-3 cells (CA-125 antigen negative), the survival rate of OVCAR-3 through a three-step treatment was remarkably decreased. The cytotoxicity results implied that paclitaxel-loaded Biotin-PEO-PLA nanoparticles were delivered more effectively to OVCAR-3 cells (CA-125 antigen positive) under the specific interaction between the biotin groups on the surface of Biotin-PEO-PLA nanoparticles and the avidin/biotinylated MAb X306/CA-125 antigen complexes on the surface of OVCAR-3 cells.

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