中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6367-6370.doi: 10.3969/j.issn.1673-8225.2010.34.023

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

改良葡萄糖微渗漏检测法评价3种根管封闭剂的封闭能力

杨军英1，王小燕1，张盛炎1，陈  珊1，林爱华2   

  1. 1中山大学附属第一医院口腔科，广东省广州市   510080；2中山大学医学统计教研室，广东省广州市   510080
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 作者简介:杨军英★，女，1964年生，福建省莆田市人，汉族，1992年中山大学毕业，硕士，副教授，主要从事牙体牙髓及牙周病方面的研究。 aliceyang2006@126.com

Comparison of sealing abilities of three different canal sealers using a modified glucose leakage assay

Yang Jun-ying1, Wang Xiao-yan1, Zhang Sheng-yan1, Chen Shan1, Lin Ai-hua2   

  1. 1 Department of Stomatology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China; 2 Department of Medical Statistics, Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China
  • Online:2010-08-20 Published:2010-08-20
  • About author:Yang Jun-ying★, Master, Associate professor, Department of Stomatology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China aliceyang2006@126.com

PDF

527

被引次数

11

摘要：

背景：应用葡萄糖作为示踪物，可对根管微渗漏进行定量检测。但传统的葡萄糖微渗漏模型存在某些不足。
目的：制备改良的葡萄糖微渗漏模型，评价GuttaFlow、AH Plus、碘仿氢氧化钙3种根管封闭剂的封闭能力。
方法：72颗单根管的前牙，于颈部截冠、镍钛根管预备后，随机分为3组各20个样本，以不同的封闭剂(GuttaFlow、AH Plus、 碘仿氢氧化钙)加牙胶尖充填根管，并设立阳性对照和阴性对照组各6个样本。应用改良葡萄糖微渗漏检测模型，以葡萄糖氧化酶比色法于第1，2，4，7，14，21，28天检测根管漏出的葡萄糖量。
结果与结论：葡萄糖渗漏量GuttaFlow组与AH Plus组相似(P > 0.05)，而碘仿氢氧化钙组的渗漏值最大，与GuttaFlow组及AH Plus组的差异均有显著性意义(P < 0.05)。实验制备的改良葡萄糖微渗漏模型，操作简单、经济实用、灵敏度较高。观察期间GuttaFlow及AH Plus封闭剂对根管的封闭能力较氢氧化钙佳。

关键词: 葡萄糖渗漏模型, 根管微渗漏, GuttaFlow, AH Plus, 碘仿氢氧化钙

Abstract:

BACKGROUND: The quantitative method using glucose as a tracer has been used for canal leakage assessment. But traditional glucose leakage model has some disadvantages.
OBJECTIVE: To prepare a modified glucose leakage model, to evaluate the sealing ability of three canal sealers of GuttaFlow, AH Plus and iodoform calcium hydroxide.
METHODS: A total of 72 extracted, human single-rooted anterior teeth were used, followed by crown truncation and nickel-titanium root canal preparation. The teeth were randomly divided into three groups (n=20), which were filled root canal with various sealers of GuttaFlow, AH Plus and iodoform calcium hydroxide. Positive and negative control groups were also set (n=6). A modified glucose leakage model was made and used for quantitative evaluation of glucose leakage at 1, 2, 4, 7, 14, 21 and 28 days by glucose oxidase colorimetric method.
RESULTS AND CONCLUSION: There were significant differences of glucose leakage between GuttaFlow group and iodoform calcium hydroxide group, and between AH Plus group and iodoform calcium hydroxide group (P < 0.05). No statistical difference was found in the glucose leakage between GuttaFlow group and AH Plus group (P > 0.05). The modified glucose leakage model is practical, sensitive, economical and accurate. GuttaFlow and AH Plus show better sealing ability than calcium hydroxide based sealer.

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