中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6175-6178.doi: 10.3969/j.issn.1673-8225.2010.33.023

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

Akt和Bad信号分子在人膝骨性关节炎中的变化

陈青植1，余跃伟2，夏  春1，张  兵3   

  1. 1福建医科大学教学医院(厦门大学附属中山医院)骨科，福建省厦门市  361004；2厦门市第三医院骨科，福建省厦门市  361100；3厦门大学医学院，福建省厦门市  361005
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 夏 春，福建医科大学教学医院(厦门大学附属中山医院)骨科，福建省厦门市 361004 chunxia99@yahoo.com.cn
  • 作者简介:陈青植★，男，1982年生，福建省厦门市人，汉族，2010年福建医科大学毕业，硕士，主要从事关节软骨基础与临床的研究。 cqz3006@gmail.com

Variation of Akt and Bad in human knee osteoarthritis

Chen Qing-zhi1, Yu Yue-wei2, Xia Chun1, Zhang Bing3   

  1. 1 Department of Orthopedics, Teaching Hospital (Zhongshan Hospital of Xiamen University), Fujian Medical University, Xiamen  361004, Fujian Province, China; 2 Department of Orthopedics, Xiamen Third Hospital, Xiamen  361100, Fujian Province, China; 3 Xiamen University Medical School, Xiamen  361005, Fujian Province, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Xia Chun, Department of Orthopedics, Teaching Hospital (Zhongshan Hospital of Xiamen University), Fujian Medical University, Xiamen 361004, Fujian Province, China chunxia99@yahoo.com.cn
  • About author:Chen Qing-zhi★, Master, Department of Orthopedics, Teaching Hospital (Zhongshan Hospital of Xiamen University), Fujian Medical University, Xiamen 361004, Fujian Province, China cqz3006@gmail.com

PDF

445

被引次数

12

摘要：

背景：已知磷脂酰肌醇三磷酸激酶(phosphatidylinositol 3-kinase，PI3K)/蛋白激酶B(protein kinase B，PKB/Akt)信号途径具有促进细胞增殖、抑制凋亡的功能，有关PI3K/Akt信号途径在骨性关节炎发病中的意义也引起关注，但缺乏对PI3K/Akt信号途径在人膝骨性关节炎软骨组织中表达及意义的研究。
目的：观察Akt、Bad信号分子在人膝骨性关节炎中的变化情况。
方法：苏木精-伊红染色观察软骨组织形态学改变，免疫组织化学技术检测31例膝骨性关节炎患者关节软骨(实验组)与10例正常人膝关节软骨(对照组)Akt及Bad的分布和表达。
结果与结论：①骨性关节炎关节软骨厚度变薄，浅层破溃；中层和深层软骨细胞排列紊乱、分布不均。②膝骨性关节炎患者中、深层软骨组织中Akt的表达均明显低于正常人关节软骨组织(P < 0.01)；Bad在骨性关节炎患者及正常人关节软骨组织内均有表达，但无显著性差异(P > 0.01)。提示软骨细胞内PI3K/Akt信号途径与骨性关节炎的发生发展密切相关，PI3K/Akt途径的变化可能是导致关节软骨破坏的主要因素之一。而Bad并没有参与骨性关节炎关节软骨细胞的生命活动，PI3K/Akt途径调节另有其他底物存在。

关键词: 骨性关节炎, 软骨, Akt, Bad, 软骨组织工程

Abstract:

BACKGROUND: Phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/Akt) signal pathway has been shown to promote cell proliferation and inhibit apoptosis. Application of PI3K/Akt signal pathway in osteoarthritis has aroused increasing attention, but its expression in human knee osteoarthritis cartilage tissue remains unclear.
OBJECTIVE: To investigate the changes of Akt, Bad pathway in knee osteoarthritis.
METHODS: The 31 samples of osteoarthritis human cartilage (experimental group) and 10 samples of normal human cartilage (control group) were selected. The normal histological technique and immunohistochemistry staining are used to detect the expression of Akt and Bad protein. 
RESULTS AND CONCLUSION: ①The osteoarthritis cartilage became thinner, and the superficial layer becomes cracked; the chondrocytes in the intercellular and deep layer were disordered in the distribution. ②The expression of Akt protein in middle and deep layers of osteoarthritis human cartilage was significantly lower than normal human cartilage (P < 0.01), but the expression of Bad was similar between normal human cartilage and osteoarthritis human cartilage (P > 0.01). It is suggested that the PI3K/Akt pathway is closely related to the occurrence and development of the knee osteoarthritis. PI3K/Akt pathway changes may a major factor inducing articular cartilage damage. Bad dose not participate chondrocyte activities of osteoarthritis, but there is other substrate exists in PI3K/Akt pathway.

中图分类号: