中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 5918-5922.doi: 10.3969/j.issn.1673-8225.2010.32.006

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐血单个核贴壁细胞与胃癌细胞的共培养：最适效靶比筛选

吴世凯1,2,3，杨  波2,4，杜  英5，胡  祥3，郑意端3，宁小琳3   

  1. 1北京大学香港科技大学深圳研修院博士后工作站，广东省深圳市 518057；2郑州大学临床医学博士后流动站，河南省郑州市 450052；3深圳市北科细胞工程研究所，广东省深圳市 518057；4郑州大学第一附属医院神经外科，河南省郑州市 450052；5郑州大学基础医学院微生物与免疫学教研室，河南省郑州市 450052
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 通讯作者: 杨波，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市 450002 yangbo96@126.com
  • 作者简介:吴世凯，男，1978年生，河北省衡水市人，汉族，2007年中国科学院水生生物研究所毕业，博士，主要从事干细胞工程化方面的研究。 shikaiwu@gmail.com
  • 基金资助:

    深圳市科技计划项目(GC200806200138A)。课题名称：深圳市干细胞工程技术研究开发中心；深圳市科技计划项目，(CJ200807210024A)，课题名称：iPS细胞的基础与应用研究；广东省科技厅产学研结合项目(2008B090500139)，课题名称：GPCRs家族C诱导神经干细胞定向分化的分子机制及其治疗与药理意义。

Cocultivation of human cord blood mononuclear cells and gastric cancer cells: Screening of optimal effector/target ratio

Wu Shi-kai 1,2,3, Yang Bo 2,4, Du Ying5, Hu Xiang3, Zheng Yi-duan3, Ning Xiao-lin3   

  1. 1 Postdoctoral Scientific Research Workstation of PKU-HKUST Shenzhen-Hong Kong Institution, Shenzhen  518057, Guangdong Province, China; 2 Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou  450052, Henan Province, China; 3 Shenzhen Beike Cell Engineering Institute, Shenzhen  518057, Guangdong Province, China; 4 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 5 Department of Microbiology and Immunology,College of Basic Medical Science, Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Yang Bo, Professor, Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Wu Shi-kai☆, Doctor, Postdoctoral Scientific Research Workstation of PKU-HKUST Shenzhen-Hong Kong Institution, Shenzhen 518057, Guangdong Province, China; Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Shenzhen Beike Cell Engineering Institute, Shenzhen 518057, Guangdong Province, China shikaiwu@gmail.com
  • Supported by:

    the Science and Technology Planning Project of Shenzhen, No. GC200806200138A*CJ200807210024A*; the Research Program of Combining Production with Education,Science and Technology Department of Guangdong Province, No. 2008B090500139*

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摘要：

背景：近年来大量研究关注脐血细胞可能对肿瘤细胞有杀伤和抑制作用，人们尝试利用脐血细胞移植抑制和杀伤肿瘤细胞，这为临床医生们提供了抗癌的新途径。
目的：探讨人脐血单个核细胞与肿瘤细胞体外共培养后，对肿瘤细胞的抑制作用。
方法：采用密度梯度离心的方法分离人脐血单个核细胞，流式检测细胞表面Marker表达，将单个核细胞与胃癌细胞进行共培养，倒置显微镜观察两种细胞共培养后细胞形态的变化，采用了乳酸脱氢酶法检测对肿瘤细胞的抑制作用，找出抗肿瘤细胞效果最好的效靶比。
结果与结论：加入脐血的贴壁单个核细胞贴壁细胞2 d后，肿瘤细胞的脱落明显，贴壁细胞实验组肿瘤细胞排列不规整，细胞密度低。酸脱氢酶法显示部分脐血贴壁细胞确有较强的抗肿瘤能力，但个体间抗肿瘤的能力差异较大，最大可相差4倍。脐血单个核贴壁细胞对肿瘤细胞有直接的杀伤作用，其最适的效靶比为2:1。

关键词: 脐血单个核细胞, 肿瘤细胞, CD34, 乳酸脱氢酶法, 干细胞

Abstract:

BACKGROUND: Many research pay attention to umbilical cord blood cells can inhibit tumor cells in recent years, people tried to suppress cancer cells by umbilical cord blood cell transplantation and these treatments may provide a new way for curing cancer.
OBJECTIVE: To investigate the effect of adhered mononuclear cells from human cord blood and gastric cancer cells after co-cultured in vitro.
METHODS: Cord blood mononuclear cells were separated by density gradient centrifugation, and the expression of surface marker of the mononuclear cells was detected by flow cytometry. Then the adhered cells and gastric cancer cells were co-cultured, and cell morphology were observed under an inverted microscope, and lactate dehydrogenase (LDH) detection was used to identify anti-tumor efficacy of cord blood mononuclear cells and to screen best effector/target ratio.
RESULTS AND CONCLUSION: At 2 days after accession of cord blood mononuclear cells, the shedding of tumor cells was obviously. In the experimental group, the tumor cells were arranged irregularly with low cell density. LDH method showed that some adherent cells from umbilical cord blood have strong anti-tumor capabilities, but the anti-tumor capacity in different individuals were varied in 4-fold. Adherent cells from umbilical cord blood have strong killing effect on tumor cells, and best anti-target ratio was 2:1.

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