中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (29): 5367-5370.doi: 10.3969/j.issn.1673-8225.2010.29.012

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胶原蛋白复合壳聚糖支架的制备及生物学性状：壳聚糖及胶原比例筛选

王  征，马云胜，穆长征，王亚光   

  1. 辽宁医学院组织学与胚胎学教研室，辽宁省锦州市   121001
  • 出版日期:2010-07-16 发布日期:2010-07-16
  • 通讯作者: 穆长征，硕士，硕士生导师，教授，辽宁医学院组织学与胚胎学教研室，辽宁省锦州市 121001 muchangzheng@sina.com
  • 作者简介:王 征★，男，1980年生，辽宁省沈阳市人，回族，辽宁医学院在读硕士，主要从事软骨组织工程方面研究。 yunshengma@126.com
  • 基金资助:

    辽宁省教育厅创新团队项目基金(2006T062)；辽宁省自然科学基金(20072201)。

Preparation and biological characteristics of collagen-chitosan composite scaffold: Proportion of chitosan and collagen

Wang Zheng, Ma Yun-sheng, Mu Chan-zheng, Wang Ya-guang   

  1. Department of Histology and Embryology, Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Mu Chang-zheng, Master, Master's tutor, Professor, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China muchangzheng@sina.com
  • About author:Wang Zheng★, Studying for master’s degree, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China yunshengma@126.com
  • Supported by:

    Innovation Team Project Fund of Liaoning Provincial Department of Education, No. 2006T062*; Liaoning Provincial Natural Science Foundation, No. 20072201*

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摘要：

背景：壳聚糖和胶原类支架已成为组织工程常用的载体材料。但是，现阶段如何能够通过调节二者比例而达到理想的细胞载体仍是目前需要解决的问题之一。
目的：调节胶原与壳聚糖二者配制比例制备支架材料，检测及对比不同比例支架材料生物学性质。
方法：制备1∶1，1∶2，1∶3，1∶4，1∶5比例的壳聚糖、Ⅰ型胶原蛋白成分，经紫外线交联后冷冻干燥，再将其用NAOH与蒸馏水进行中和，二次冻干制备好支架。观察不同比例支架的材料性质、孔隙率、降解率、溶胀率；扫描电镜观察孔径的大小及形态。
结果与结论：在壳聚糖含量固定，支架的大体孔径经统计比较差异有显著性意义(P < 0.05)。1∶3比例制备的孔径最大，达到(298.0±36.0) μm；支架总体的孔隙率为93.9%~97.5%，胶原比例的增加对支架孔隙率的影响较轻微。各组支架的溶胀率可达到80%左右，支架的溶胀程度随胶原比例增加而减少。在支架的降解率随着胶原比例增加而增加，而壳聚糖含量越高，降解速度越慢。结果证实，通过紫外光交联法，按照1∶3 配比的胶原和壳聚糖的支架材料更加适合软骨组织工程的要求。

关键词: 壳聚糖, 胶原蛋白, 支架, 紫外线交联, 组织工程

Abstract:

BACKGROUND: Chitosan and collagen scaffolds have been used as a common carrier material for tissue engineering. However, how to obtain an ideal cell carrier by adjusting the ratio between the two is still an unsolved problem.
OBJECTIVE: To adjust the proportion of collagen and chitosan for complex scaffolds, to detect and compare biological properties of scaffolds prepared at various proportions.
METHODS: Chitosan and type collagen protein were added by ratio 1:1, 1:2, 1:3, 1:4, 1:5. The materials were freeze-dried after UV cross-linking, then neutralized by NaOH and distilled water, followed by additional freeze-drying. The scaffolds at different proportions were observed for the properties, the degradation rate, the porosity, and swelling ratio. The size and distribution of the holes were detected under scanning electron microscopy.
RESULTS AND CONCLUSION: At a fixed proportion of chitosan, there were significant differences in scaffold aperture by the statistical comparison (P < 0.05). The aperture diameter of the scaffold prepared at 1:3 ratio was the largest, (298.0±36.0) μm; the overall porosity was up to 93.9%-97.5%, the increasing ratio of collagen showed less impact on the porosity. The swelling rate reached about 80%, swelling degree of the scaffold was at an inverse proportion with the increase in the proportion of collagen. The degradation rate of the scaffold was increased with the increasing proportion of collagen. The higher chitosan concentration is, the slower degradation is. By means of UV cross-linking method, 1:3 ratio of collagen-chitosan scaffolds is more suitable for cartilage tissue engineering.

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