中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5256-5259.doi: 10.3969/j.issn.1673-8225.2010.28.030

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

组织工程气管的构成和构建方法

王林毛，高  文，陈  昶   

  1. 同济大学附属上海市肺科医院胸外科，上海市 200433
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 高文，教授，主任医师，上海市肺科医院胸外科，上海市 200433 gaowen5921@163.com
  • 作者简介:王林毛★，男，1984年生，江苏省盐城市人，江苏大学在读硕士，主要从事气管移植基础和临床方面的研究。
  • 基金资助:

    上海市科学技术委员会(09411963300)。

Composition and construction method of tissue-engineered trachea 

Wang Lin-mao, Gao Wen, Chen Chang   

  1. Department of Chest Surgery, Affiliated Shanghai Pulmonary Hospital of Tongji University, Shanghai  200433, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Gao Wen, Professor, Chief physician, Department of Chest Surgery, Affiliated Shanghai Pulmonary Hospital of Tongji University, Shanghai 200433, China gaowen5921@163.com
  • About author:Wang Lin-mao★, Studying for master’s degree, Department of Chest Surgery, Affiliated Shanghai Pulmonary Hospital of Tongji University, Shanghai 200433, China
  • Supported by:

    the Shanghai Science and Technology Committee, No. 09411963300*

PDF

338

被引次数

1

摘要：

背景：当气管切除长度超过6 cm，因吻合口张力大易产生吻合口瘘，这时需要进行气管重建，但无论是同种异体移植，自体组织移植和人工替代物等方法效果均不理想。组织工程学是近年来的研究热点，气管组织工程学虽然起步较晚，但已经取得一些重要成果，显示出良好的前景。
目的：从支架材料、种子细胞、生长因子、立体结构重建、以及临床应用等方面，全面了解组织工程气管的构成和构建方法。
方法：以“气管移植(tracheal transplantation)，组织工程气管(tissue-engineered trachea)”为检索词，应用计算机检索维普数据库，Pubmed数据库，Elsevier数据库2000/2009有关文章。纳入与组织工程气管的构成和构建方法密切相关的文献，排除重复性研究。
结果与结论：共检索到279篇文献，排除无关重复的文献，保留30篇文献进行综述。目前组织工程气管应用的支架材料主要为合成材料，纳米材料被认为是最具潜能的；种子细胞来源有软骨细胞和气管上皮细胞。旋转生物反应器可作为体外构建组织工程化气管软骨的可行的培养方法，生长因子的应用可诱导、加快和增强软骨形成。组织工程气管由于供体组织少，不易发生排斥反应，具有良好的应用前景。但还存在组织工程化气管的质地和延展性、管腔内壁上皮化和组织工程化气管的血管化等问题有待进一步解决。

关键词: 组织工程气管, 支架材料, 种子细胞, 生长因子, 立体结构重建, 临床应用

Abstract:

BACKGROUND: When a more than 6-cm trachea is removed, the anastomotic fistula would occur due to great tension, thus, trachea reconstruction should be performed. However, the therapeutic effect of allograft transplantation, autotransplantation, and artificial substitutes are unsatisfactory. Tissue engineering is a developing research spot in recent years, and tissue-engineered trachea has exhibits favorable prospect in treating anastomotic fistula.
OBJECTIVE: To understand the composition and construction method of tissue-engineered trachea from the aspects of scaffold materials, seed cells, growth factor, reconstruction of stereochemical structure, and clinical applications.
METHODS: The databases of VIP, Pubmed and Elsevier were searched by computer with the key words of “tracheal transplantation and tissue-engineered trachea”. The articles related to construction method of tissue-engineered trachea and published between 2000 and 2009 were selected. The repetitive literatures were excluded.
RESULTS AND CONCLUSION: Totally 279 literatures were initial searched, and 30 articles were included for further study. Currently, most of scaffold for tissue-engineered trachea were made from synthetic materials, and nano-material was thought to be the most potential material. Seed cells were resourced from chondrocytes and tracheal epithelial cells. Tissue-engineered trachea chondrocytes could be constructed in vitro by using rotary cell culture system, and the adding of growth factors could induce, accelerate and enhance chondrosis. Tissue-engineered trachea has widely prospect for its less donor tissue and free from rejection. However, there are still problems encounter its application. 

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