中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5006-5009.doi: 10.3969/j.issn.1673-8225.2010.27.015

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

不同分化程度卵巢癌细胞中胚胎干细胞相关通路的基因集富集分析

叶  云1,2，王桂平1,3，杨晓勤1，尹  曦1，梁  爽1，郑文岭1，马文丽1   

  1. 1南方医科大学基因工程研究所，广东省广州市 510515，2广西工学院生物与化学工程系， 广西壮族自治区柳州市  545006；3广州医学院护理学院，广东省广州市 510182
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 马文丽，教授，博士生导师，南方医科大学基因工程研究所，广东省广州市 510515 wenli@fimmu.com
  • 作者简介:叶 云☆，男，1978年生，广西壮族自治区灵山县人，南方医科大学在读博士，讲师，主要从事基因芯片的临床应用研究。 yeemvan@126.com
  • 基金资助:

    广东省生物芯片重点实验室基金(2004B60144)资助项目。

Analyses of gene sets associated with embryonic stem cells in different histological grades of ovarian cancer

Ye Yun 1,2, Wang Gui-ping 1,3, Yang Xiao-qin1, Yin Xi1, Liang Shuang1, Zheng Wen-ling1, Ma Wen-li1   

  1. 1 Institute of Genetic Engineering, Southern Medical University, Guangzhou   510515, Guangdong Province, China; 2 Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou   545006, Guangxi Zhuang Autonomous Region, China; 3 Nursing School, Guangzhou Medical College, Guangzhou   510182, Guangdong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Ma Wen-li, Professor, Doctoral supervisor, Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, Guangdong Province, China wenli@fimmu.com
  • About author:Ye Yun☆, Studying for doctorate, Lecturer, Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, Guangxi Zhuang Autonomous Region, China yeemvan@126.com
  • Supported by:

    the Biochip Key Laboratory of Guangdong Province, No. 2004B60144*

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被引次数

1

摘要：

背景：近年来一些研究发现肿瘤细胞具有与干细胞类似的特征，因此，控制干细胞功能的某些基因调控网络，可能也在某些肿瘤中同样发挥作用。
目的：观察不同分化程度卵巢癌的分子特征，获得与胚胎干细胞相关基因的表达情况。
方法：从美国国立生物信息中心(NCBI)共享数据库GEO下载原始基因芯片文件，登录号为GSE2109，选取卵巢癌样本作为研究材料。去除临床指标缺失的样本，按照组织学分级将卵巢癌样本分为高分化和低分化两组。原始数据经dChip进行质量检验和标准化，获得基因表达的矩阵。收集胚胎干细胞相关的基因集、生物学过程和KEGG通路在不同分化的卵巢癌中的富集情况，用GSEA进行基因集富集分析。
结果与结论：选取了13个与胚胎干细胞相关的基因集，低分化的卵巢癌细胞中有9个基因集上调，PRC2靶点相关的4个基因集下调，说明与胚胎干细胞相关的基因集大多在低分化的卵巢癌细胞中上调。并且在低分化卵巢癌细胞显著上调的都是与细胞周期、细胞分裂、DNA复制等与增殖相关的通路。基因表达谱分析表明低分化的卵巢癌与胚胎干细胞具有相似的分子特征，这可以为卵巢癌的早期诊断和治疗提供新的思路。

关键词: 表达谱, 胚胎干细胞, 分化, 卵巢癌, 基因通路, 富集基因集

Abstract:

BACKGROUND: Recent studies have found that tumor cells have the characteristics of stem cells. Therefore, the gene regulation network that controls the function of stem cells may play important roles in some tumors.
OBJECTIVE: To study the molecular trait of ovary cancer in different grades, and to obtain the expression of gene sets associated with embryonic stem cells in ovary cancer.
METHODS: Gene expression profile data of ovarian cancer (accession number GSE2109) were obtained from Gene Expression Omnibus (GEO) database of National Center for Biotechnology Information. Ovarian cancer samples served as study materials. Samples without clinical data were excluded and then separated into two groups (high differentiation and low differentiation) according to histological grade. Expression value of samples was obtained after raw profile data were normalized and quality control by dChip. Matrix of gene expression was obtained. The enrichment of gene sets, biological process and KEGG pathway were analyzed in ovarian cancer of various differentiations by GSEA software.
RESULTS AND CONCLUSION: A total of 13 gene sets associated with embryonic stem cells, 9 upregulated in poorly differentiated tumors, whereas 4 associated with PRC2 target downregulated. These suggested that gene sets associated with embryonic stem cells upregulated in poorly differentiated tumors. And pathways related cell cycle, cell division and DNA replication also enrich in poorly differentiated tumors. Gene expression profile revealed poorly differentiated ovary tumors possess the similar molecular trait with embryonic stem cells. These analyses may benefit further investigations of early diagnosis and treatment of ovarian cancer.

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