中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5001-5005.doi: 10.3969/j.issn.1673-8225.2010.27.014

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

诱导人孤雌胚胎干细胞分化为胰岛样细胞团

周  静1,2，李  进1,2，林  戈1,2，卢光琇1,2   

  1. 1中南大学生殖与干细胞研究所，湖南省长沙市  410078；2国家干细胞工程研究中心，湖南省长沙市  410078
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 卢光琇，中南大学生殖与干细胞研究所，湖南省长沙市 410078；国家干细胞工程研究中心，湖南省长沙市 410078
  • 作者简介:周 静☆，女，1982年生，江西省鹰潭市人，汉族，中南大学生殖与干细胞研究所在读博士，主要从事人胚胎干细胞工程研究。 zhoujing12 @gmail.com
  • 基金资助:

    国家“973”项目(2005CB522705)，课题名称：同种异体干细胞研究与通用胚胎干细胞库的建立。国家“863”项目(2006AA02A102)，课题名称：我国人类(疾病)胚胎干细胞库的建立与应用。国家自然科学基金项目(30800659)，课题名称：不同人胚胎干细胞系向内胚层细胞分化能力出现差异的表观遗传学机制探讨。

Inducing human parthenogenetic embryonic stem cells into islet-like clusters

Zhou Jing 1,2, Li Jin 1,2, Lin Ge 1,2, Lu Guang-xiu 1,2   

  1. 1 Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha   410078, Hunan Province, China; 2 National Research and Engineering Center of Human Stem Cells, Changsha    410078, Hunan Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Lu Guang-xiu, Professor, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, Hunan Province, China; National Research and Engineering Center of Human Stem Cells, Changsha 410078, Hunan Province, China lugxdirector@yahoo.com.
  • About author:Zhou Jing☆, Studying for doctorate, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, Hunan Province, China; National Research and Engineering Center of Human Stem Cells, Changsha 410078, Hunan Province, China zhoujing12 @gmail.com
  • Supported by:

    the Hi-Tech Research and Development of China (863 program), No. 2006AA02A102*; the National Basic Research Program of China (973 program), No.2005CB522705*; the National Natural Science Foundation of China, No. 30800659*

PDF

345

被引次数

19

摘要：

背景：随着人类孤雌胚胎干细胞系的成功建立，对该细胞的进一步分化功能研究成为新的研究热点。
目的：研究体外培养的人类孤雌胚胎干细胞诱导分化为胰岛样细胞团的潜能。
方法：自主建系孤雌来源人胚胎干细胞和正常来源人胚胎干细胞各1株，运用基于胰腺体内发育规律的改良5阶段诱导法诱导人孤雌胚胎干细胞为胰岛样细胞团，加入不同生长因子及诱导试剂对人胚胎干细胞分5阶段序贯培养。
结果与结论：终末分化细胞光镜下呈团状聚集，RT-PCR、免疫荧光染色检测分化细胞表达胰岛细胞特征性的基因与蛋白。胰岛素释放实验提示获得的细胞具有胰岛样生化功能。由人类孤雌胚胎干细胞来源的胰岛样细胞团具备胰岛的基本特征，是未来治疗1型糖尿病的可用材料。

关键词: 人类孤雌胚胎干细胞, 胰岛样细胞团, 诱导分化, 糖尿病, 干细胞

Abstract:

BACKGROUND: Following the successful establishment of human parthenogenetic embryonic stem (hpES) cell lines, the further functional study becomes hot spot.
OBJECTIVE: To investigate the potential differentiation ability to islet-like clusters from hpES cell lines.
METHODS: One self-established hpES cell line and one normal hpES cell line were obtained. We adopted a modified 5-stages protocol which based on the development rule of pancreas to induce hpES cells into islet-like clusters.
RESULTS AND CONCLUSION: The terminal differentiated cells gathered into islet-like clusters. The results from immunohistochemistry and RT-PCR showed that the islet-like clusters expressed the islet specific hormones and functional markers. Insulin release test indicated that the clusters had the biochemical function of islet. The islet-like clusters derived from phES cells had the basic characteristics of islet, which would be a reliable material to treat type I diabetes mellitus.

中图分类号: