中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (26): 4827-4830.doi: 10.3969/j.issn.1673-8225.2010.26.019

• 骨与关节图像与影像 bone and joint imaging • 上一篇    下一篇

光学相干层析成像技术对光滑面早期牙釉质人工龋的定量检测

李燕妮1，王冠华1，姚 晖1，孟 卓2，代晓华1，梁 雨2，连小丽1，万木森2，张林朴1，石博雅2，姚晓天2，刘铁根2   

  1. 1天津市口腔医院暨南开大学附属口腔医院实验研究中心，天津市300041；
    2天津大学精密仪器与光电工程学院，天津市  300072
  • 出版日期:2010-06-25 发布日期:2010-06-25
  • 通讯作者: 姚 晖，主任医师，硕士生导师，天津市口腔医院暨南研大学附属口腔医院实验研究中心，天津市300041 yaoh2k@163. com.
  • 作者简介:李燕妮，女，1980年生，河北省张家口市人，汉族，2007年天津医科大学毕业，硕士，医师，主要从事肿瘤分子病理、口腔医学研究。 shadeofsmile@ 126.com.
  • 基金资助:

    国家自然科学基金面上项目(30770597)，
    天津市口腔医院重点科技基金资助项目

Quantitative detection of early emanel artificial caries on the smooth surface using optical coherence tomography  

Li Yan-ni1, Wang Guan-hua1, Yao Hui1, Meng Zhuo2, Dai Xiao-hua1, Liang Yu2, Lian Xiao-li1, Wan Mu-sen2, Zhang Lin-pu1,
Shi Bo-ya2, Yao Xiao-tian2, Liu Tie-gen2   

  1. 1Research Center of Stomatological Hospital, Nankai University, Tianjin Stomatological Hospital, Tianjin   300041, China;
    2College of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin   300072, China
  • Online:2010-06-25 Published:2010-06-25
  • Contact: Yao Hui, Chief physician, Master’s supervisor, Research Center of Stomatological Hospital, Nankai University, Tianjin Stomatological Hospital, Tianjin 300041, China yaoh2k@163.com
  • About author:Li Yan-ni, Master, Physician, Research Center of Stomatological Hospital, Nankai University, Tianjin Stomatological Hospital, Tianjin 300041, China shadeofsmile@ 126.com
  • Supported by:

    the General Program of the National Natural Science Foundation of China, No. 30770597*;
    the Key Science and Technology Foundation of Tianjin Stomatological Hospital*

PDF

459

被引次数

12

摘要：

背景：光学相干层析成像技术是一种高分辨率、非创伤性成像技术，它能够利用近红外光产生牙体组织的深度解析图像，并通过检测背向散射信号来对龋损进行定量检测。
目的：通过与偏振光显微镜的对比，验证光学相干层析成像技术对光滑面早期釉质龋的成像能力及对龋损深度定量检测的准确性。
方法：以6岁龄牛下切牙为样本，采用pH循环法建立光滑面早期龋人工模型，分别应用全光纤光学相干层析成像技术和偏振光显微镜进行检测，比较两种方法获得龋损图像和深度值，进行方差分析和相关性分析。
结果与结论：光学相干层析成像系统能够清晰成像光滑面早期龋，表层下脱矿区域信号显著增强，并且测得的脱矿深度值与偏振光显微镜结果一致， 即该系统对深度的测量具有较高的准确性，可用于对早期龋纵深进展的评估。光学相干层析成像技术测得的人工龋模型开窗区的深度值与脱矿时间无相关性(P > 0.05)。计算开窗区平均深度值与开窗区外平均深度值的差值即深度变化值(△D)，则△D与脱矿时间呈正相关(r=0.954，P < 0.05)。结果提示了这种无创、高分辨率的光学成像技术在早期龋的定量研究和临床诊断方面具有广阔的应用前景。

关键词: 光学相干层析成像, 早期人工龋, 光滑面, 定量检测, 数字化图像与影像技术

Abstract:

BACKGROUND: Optical coherence tomography (OCT) is a noninvasive imaging modality with high resolution. It can produce depth-resolved images of tooth utilizing near-infrared light and quantitatively examine the caries by detecting the backscatter signals.
OBJECTIVE: To explore the ability of imaging the early enamel caries on the smooth surface and the accuracy of quantifying the caries depths with OCT via the comparative study of OCT and polarized light microscope.
METHODS: The early enamel caries model on the smooth surface was prepared through a pH cycling model with calf (< 6 years old) lower incisor as sample, and detected by an all fiber-based OCT and polarized light microscope. The images and caries depths from both methods were compared using ANOVA and Pearson correlation.
RESULTS AND CONCLUSION: OCT could image the early enamel caries. The reflectivity within the demineralized subsurface enamel was markedly greater than the sound enamel in the OCT images. There were no differences in caries depths between OCT and polarized light microscope. OCT, which could accurately quantify the caries depths, may be used to evaluate the longitudinal progress of the early caries. There was no correlation between the lesion depth and demineralization time (P > 0.05). The altered depths between interior and exterior of the test region were calculated. The increased depths were correlated with the demineralization time (r = 0.954, P < 0.05). Results suggested the wide prospect of this nondestructive and high-resolution method on the research and clinical diagnosis of early caries.

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