中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4568-4572.doi: 10.3969/j.issn.1673-8225.2010.25.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

脱钙骨/聚乳酸重组人工骨的体内生物学特性

刘金标，黄  钢，潘显明，马泽辉，邓  冰，邓少林，张  波，周劲松，伍红桦   

  1. 解放军成都军区总医院骨科，四川省成都市  610083
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 作者简介:刘金标★，男，1970年生，江西省高安市人，汉族，2001年解放军第一军医大学毕业，硕士，副主任医师，主要从事关节外科及人工骨材料研究。 liujinbiao@medmail.com.cn
  • 基金资助:

    成都军区总医院院管课题(03Y007)。

Biological properties of demineralized bone/polylactic acid composite artificial bone in vivo

Liu Jin-biao, Huang Gang, Pan Xian-ming, Ma Ze-hui, Deng Bing, Deng Shao-lin, Zhang Bo, Zhou Jin-song, Wu Hong-hua   

  1. Department of Orthopaedics, General Hospital of Chengdu Military Area Command of Chinese PLA, Chengdu  610083, Sichuan Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author: Liu Jin-biao, Master, Associate chief physician, Department of Orthopaedics, General Hospital of Chengdu Military Area Command of Chinese PLA, Chengdu 610083, Sichuan Province, China liujinbiao@medmail.com.cn
  • Supported by:

    a Grant of General Hospital of Chengdu Military Area Command of Chinese PLA, No. 03Y007*

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306

被引次数

3

摘要：

背景：前期实验证实脱钙骨/聚乳酸重组人工骨不仅孔隙率、孔径、机械强度等性能符合人工骨材料的要求，并且结合了两种材料的优势，具有明显的促进细胞贴附、增殖作用。
目的：进一步探讨脱钙骨/聚乳酸重组人工骨在体内的生物相容性、降解情况及成骨特点，评价其性能。
方法：将脱钙骨/聚乳酸重组人工骨随机植入成年新西兰白兔1.5 cm的桡骨缺损及一侧臀肌内，并设立不植入任何材料的空白对照组。观察脱钙骨/聚乳酸重组人工骨植入后动物局部反应，检测血钙值；植入后6，8，12，16周取骨缺损处标本作X射线、骨矿含量、大体标本及组织形态学观察，臀肌处标本仅作组织形态学观察，分析不同时期组织反应、骨缺损修复及材料降解情况。
结果与结论：脱钙骨/聚乳酸重组人工骨植入后无明显的局部不良反应，血钙值无明显变化；骨缺损内骨矿含量在植入6~16周内升高幅度明显高于空白对照组；X射线、大体标本及组织形态学观察显示，脱钙骨/聚乳酸重组人工骨的成骨方式主要是骨传导成骨，至植入后16周时骨缺损基本修复，而空白对照骨缺损断端仅有少量骨修复，形成骨不连；脱钙骨/聚乳酸重组人工骨植入后即开始其降解过程，12周以后材料周围出现较多吞噬有材料颗粒的巨噬细胞和多核巨细胞，16周时仍有部分材料未降解吸收。结果证实脱钙骨/聚乳酸重组人工骨具备良好的生物相容性和骨传导成骨能力，可以在体内逐渐发生生物降解。

关键词: 脱钙骨, 骨替代材料, 聚乳酸, 降解, 生物相容性材料

Abstract:

BACKGROUND: Previous studies have exhibited that demineralized bone/polylactic acid composite artificial bone (BPCB) meets the requirements of artificial bone material in its porosity rate, pore size, mechanical strengths and possess both quality of demineralized bone and polylactic acid. BPCB can facilitate the adhension and proliferation of human osteoblasts significantly.
OBJECTIVE: To further investigate compatibility, degradation and bone formation of BPCB in vivo.
METHODS: Bone defects with 1.5 cm in length were filled with BPCB or nothing randomly soon after they were produced at both radii of adult New Zealand rabbits. Furthermore, BPCB granules were embedded in the same side gluteus. The animals’ local and whole body response to the implants were observed after the operation, and their serum calcium levels were detected. At 6, 8, 12 and 16 weeks postoperation, samples at bone defect region were evaluated by X-ray, bone mineral content (BMC), general sample and underwent histomorphological observation. Samples from gluteus only received histomorphological observation. Tissues response, new bone formation in the defects and degradation of the implants were analyzed at different phases.
RESULTS AND CONCLUSION: Following BPCB transplantation, no significant local adverse reactions and no significant changes in serum calcium levels were found. BMC in the defects following BPCB grafts was increased more rapidly than the blank control group during 6-16 weeks. Gross, X-ray and histological observations revealed that BPCB was osteoconductive. At 16 weeks, the defects nearly got completely repaired while the blank control group remained nonunion. Biodegradation of the implants was observed early following implantation, and there were many macrophages and multinuclear giant cells swallowed with particles of BPCB surrounding the implants at 12 weeks, but there were still remnants of BPCB in the defects at 16 weeks. BPCB possesses good biocompatibility, bone conduction and bone formation ability, and biodegradation occurs.

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