中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (21): 3811-3814.doi: 10.3969/j.issn.1673-8225.2010.21.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

藻酸钙凝珠复合自体软骨细胞修复成年兔关节软骨缺损

吴  迪1，赵智君2，朱晓松1，杨  浩1，李  青1，马  珂1   

  1. 1昆明医学院第一附属医院骨科，云南省昆明市 650032；2昆明医学院第一临床学院，云南省昆明市 650031
  • 出版日期:2010-05-21 发布日期:2010-05-21
  • 通讯作者: 赵智君，在读硕士，昆明医学院第一临床学院，云南省昆明市 650031 zzjdq@163.com
  • 作者简介:吴 迪☆，博士，主治医师，主要从事骨与关节创伤研究。 xywudi@sohu.com
  • 基金资助:

    云南省应用基础研究面上项目(20088C117M)。课题名称:  海藻糖复合自体软骨细胞修复负重关节软骨的实验研究。

Calcium alginate beads combined with autologous chondrocyte for repair of adult rabbit articular cartilage injury

Wu Di1, Zhao Zhi-jun2, Zhu Xiao-song1, Yang Hao1, Li Qing1, Ma Ke1   

  1. 1 Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming   650032, Yunnan Province, China; 2 the First Clinical College of Kunming Medical College, Kunming   650031, Yunnan Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Zhao Zhi-jun, Studying for master’s degree, the First Clinical College of Kunming Medical College, Kunming 650031, Yunnan Province, China zzjdq@163.com
  • About author:Wu Di☆, Doctor, Attending physician, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China xywud@sohu.com
  • Supported by:

    General Program of Applied Basic Study of Yunnan Province, No. 20088C117M*

PDF

423

被引次数

9

摘要：

背景：软骨缺损的修复一直是骨科界的难题之一，近几年发展起来的应用组织工程学技术修复软骨缺损的方法，逐渐被认为是一种有希望治疗关节软骨缺损的手段。
目的：应用藻酸钙凝珠-自体软骨细胞移植修复兔膝关节软骨缺损，观察损伤近期修复情况。
方法：成年新西兰大白兔30只，左膝取软骨细胞，右膝造模。采用体外培养后的第3代自体软骨细胞复合海藻酸钠凝胶，混入CaCl2溶液中制成串珠状凝珠，体外培养4周。实验组植入海藻酸钙凝珠-自体软骨细胞复合物，对照组植入不含细胞的海藻酸钙凝珠，空白组不做任何处理。
结果与结论：纳入新西兰大白兔30只，除其中1 只术后单侧膝关节僵硬强直外，其余均未见手术后关节活动障碍，所有动物术后无感染，无死亡。苏木精-伊红染色可见，实验组修复组织以透明软骨为主，对照组以纤维软骨修复为主，空白组修复不明显，以纤维软组织修复为主。免疫组织化学染色可见实验组修复组织以表达Ⅱ型胶原为主，对照组Ⅱ型胶原免疫组化着色较淡，Ⅱ型胶原表达量少。空白组修复组织不表达Ⅱ型胶原。结果提示藻酸钙凝珠-自体软骨细胞修复软骨缺损有较好效果，表明自体软骨细胞复合藻酸钙凝珠能为软骨组织工程提供一种很好的思路。

关键词: 藻酸钙凝珠, 软骨细胞, 软骨组织工程, 修复, 生物材料

Abstract:

BACKGROUND: Cartilage defect is always one of the difficulties in the field of orthopedics. Tissue engineering technology for repairing cartilage defects, a recently developed method, has been gradually considered a promising means of articular cartilage defects treatment.
OBJECTIVE: To observe the short-term effect of rabbit articular cartilage defects repaired by calcium alginate beads-autologous chondrocyte transplantation.
METHODS: Thirty adult New Zealand white rabbits were adopted in this study. The chondrocytes were harvested from their left knee of every group, and the model of articular cartilage injury was manufactured in the right knee. The in vitro cultured autologous chondrocytes of the third generation were composited alginate gel, then mixed with CaCl2 solution to prepare bead-like condensate, cultured in vitro for 4 weeks. The holes of the experimental group were implanted with calcium alginate beads-autologous chondrocyte composite, the control group with calcium alginate beads without cells, and the blank control group without any treatment.
RESULTS AND CONCLUSION: All 30 New Zealand white rabbits were included. With the exception of 1 rabbit exhibited unilateral ankylosis of postoperative knee stiffness, the other joint activities after surgery had no obstacles. All the animals showed no postoperative infection or deaths. Hematoxylin-eosin stain showed that, repair tissue mainly was hyaline cartilage in the experimental group, and fiber-based cartilage in the control group, but repairing effect was not obvious in the blank group, mainly fibrous soft tissue repair. Immunohistochemical staining demonstrated repair tissue expression was type Ⅱ collagen expression in the experimental group, in the control group, type Ⅱ collagen was stained lightly, with less type Ⅱ collagen expression. The repair tissue in the blank group did not express type Ⅱ collagen. Results revealed that calcium alginate beads-autologous chondrocyte is effective in the repair of cartilage defects, indicating that autologous chondrocytes combined with calcium alginate beads can provide a better idea for cartilage tissue engineering.

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