中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (21): 3815-3818.doi: 10.3969/j.issn.1673-8225.2010.21.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

重组腺病毒介导的骨形态发生蛋白9与骨形态发生蛋白2复合纳米羟基磷灰石/聚酰胺多孔支架修复桡骨缺损的比较

卢小娟1，赵迎泽1，文  巍1，张  婷1，陈恒君2，周  炜3，冯  涛3，康  权4   

  1. 1重庆医科大学临床检验教育部重点实验室，重庆市  400014；2重庆医科大学附属第一医院皮肤科，重庆市  400014；3重庆医科大学分子医学与肿瘤研究中心，重庆市  400014；4重庆医科大学附属儿童医院儿外科，重庆市  400016
  • 出版日期:2010-05-21 发布日期:2010-05-21
  • 通讯作者: 康 权，博士，副教授，重庆医科大学附属儿童医院儿外科，重庆市 400016 kq1028@yahoo.com
  • 作者简介:卢小娟★，女，1984年生，重庆市人，汉族，重庆医科大学在读硕士，主要从事骨形态发生蛋白9对成骨作用的体内和体外研究工作。 piaopiaogangan@163.com
  • 基金资助:

    国家自然科学基金项目资助(30600625/C1607)。

Recombinant adenovirus-mediated bone morphogenetic protein 9 versus bone morphogenetic protein 2 combined with nano-hydroxyapatite/polyamide composites in repair of radial defects

Lu Xiao-juan1, Zhao Ying-ze1, Wen Wei1, Zhang Ting1, Chen Heng-jun2, Zhou Wei3, Feng Tao3, Kang Quan4   

  1. 1 Key Laboratory of Clinical Laboratory, Ministry of Education, Chongqing Medical University, Chongqing  400014, China; 2 Department of Dermatology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400014, China; 3 Molecular Medicine and Cancer Research Centre, Chongqing Medical University, Chongqing  400014, China; 4 Department of Pediatric Surgery, Children’s Hospital of Chongqing Medical University, Chongqing  400016, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Kang Quan, Doctor, Associate professor, Department of Pediatric Surgery, Children’s Hospital of Chongqing Medical University, Chongqing 400016, China
  • About author:Lu Xiao-juan★, Studying for master’s degree, Key Laboratory of Clinical Laboratory, Ministry of Education, Chongqing Medical University, Chongqing 400014, China piaopiaogangan@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600625/C1607*　

PDF

427

被引次数

13

摘要：

背景：课题组采用新的重组腺病毒介导的基因表达手段，与传统方法比较具有高效、方便、安全等优点，可作为进入临床基因治疗应用的潜在有力手段。
目的：比较腺病毒介导的人骨形态发生蛋白9(Adv-hBMP-9)与腺病毒介导的人骨形态发生蛋白2(Adv-hBMP-2)分别复合纳米羟基磷灰石/聚酰胺多孔支架材料修复重建桡骨缺损的效果。
方法：新西兰白兔36只，随机分成3组，制成双侧桡骨中段13 mm骨缺损模型。分别植入Adv-hBMP-9+纳米羟基磷灰      石/聚酰胺骨、Adv-hBMP-2+纳米羟基磷灰石/聚酰胺骨、Adv-GFP+羟基磷灰石/聚酰胺骨。植入后进行大体观察、X射线摄片、组织学检测。
结果与结论：BMP-9组骨缺损完全修复，BMP-2组骨缺损部分修复，而GFP对照组骨缺损修复明显欠佳。提示重组腺病毒介导的BMP-9对桡骨骨缺损后的成骨修复作用强于BMP-2。

关键词: 人骨形态发生蛋白9, 人骨形态发生蛋白2, 腺病毒, 纳米羟基磷灰石, 聚酰胺, 骨缺损, 成骨作用

Abstract:

BACKGROUND: Compared with the traditional method, this paper applies a new method of recombinant adenovirus-mediated gene expression with the advantages of high efficiency, convenience and safety, which can serve as powerful means for gene therapy.
OBJECTIVE: To compare the effect of adenovirus-mediated human bone morphogenetic protein 9 (Adv-hBMP-9) and adenovirus-mediated human bone morphogenetic protein 2 (Adv-hBMP-2) nano-hydroxyapatite / polyamide complex on reconstruction of bone defects.
METHODS: A total of 36 New Zealand rabbits were randomly divided into 3 groups and prepared for 13-mm bone defect models at the bilateral middle radius. Adv-hBMP9 + nano-hydroxyapatite / polyamide bone, Adv-hBMP-2 + nano-hydroxyapatite / polyamide bone, and Adv-GFP + hydroxyapatite / polyamide bone were implanted into the defects in each group. General observation, X-ray photographs and histological sections were carried out separately.
RESULTS AND CONCLUSION: Bone defects were completely repaired in the BMP-9 group; partly repaired in the BMP-2 group; however, reparation of bone defects was obviously poor in the GFP control group. The results revealed that effect of recombinant Adv-hBMP-9 is stronger than Adv-hBMP-2 on repairing osteogenesis after radial defects.

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