中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 6847-6850.doi: 10.3969/j.issn.1673-8225.2010.15.044

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    

胎儿颌面部皮肤透明质酸的含量分析

李向军1，肖铁朋2，任贵云1，董福生1   

  1. 1河北医科大学口腔医院口腔颌面外科，河北省石家庄市   050017；2 河北医科大学第二医院口腔正畸科，河北省石家庄市   050011
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 董福生，教授，博士生导师，河北医科大学口腔医院口腔颌面外科，河北省石家庄市人 050017
  • 作者简介:李向军☆，女，1973年生，河北省石家庄市人，汉族，日本信州大学医学部在读博士，主治医师，主要从事口腔颌面外科临床与基础研究工作。
  • 基金资助:

    课题受到河北省科技攻关计划项目(04276101D-81)及河北省博士资金资助项目(03547003D)的资助。

Hyaluronic acid content in fetal maxillofacial skin

Li Xiang-jun1, Xiao Tie-peng2, Ren Gui-yun1, Dong Fu-sheng1   

  1. 1 Department of Oral Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang   050017, Hebei Province, China; 2 Department of Orthodontics, Second Hospital of Hebei Medical University, Shijiazhuang   050011, Hebei Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Dong Fu-sheng, Professor, Doctoral supervisor, Department of Oral Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China hblxj2000@yahoo.com.cn
  • About author:Li Xiang-jun☆, Studying for doctorate, Attending physician, Department of Oral Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China hblxj2000@yahoo.com.cn
  • Supported by:

    Hebei Provincial Programs for Science and Technology Development, No. 04276101D-81*; Special Foundation of Doctoral Research of Hebei Province, No. 03547003D*

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被引次数

4

摘要：

背景：伤口处细胞外基质中高含量的透明质酸对瘢痕形成有抑制作用，胚胎皮肤切口呈现无瘢痕愈合，但对于胎儿皮肤中透明质酸的含量未见报道。
目的：观察胎儿颌面部皮肤中透明质酸含量及其变化趋势，并比较与其他部位的差异。
方法：取32例3~8月龄引产胎儿的颌面部、背部、腹部及大腿内侧四处皮肤，按取后液氮速冻，按部位分为不同月龄组。标本称质量后剪碎，加生理盐水制成组织匀浆，取上清液，用放射免疫法测定其中透明质酸含量。
结果与结论：3~8月龄胎儿颌面部皮肤中的透明质酸平均质量分数为(303.85±80.28) μg/g，腹部皮肤为(308.09±61.17) μg/g，背部皮肤为(276.38±58.52) μg/g，腿部皮肤为(304.27±60.51) μg/g。胎儿颌面部及背部皮肤中透明质酸含量3，4，5月龄高于6，7，8月龄(P < 0.05)，腹部皮肤4，5月龄高于6，7，8月龄(P < 0.05)，腿部皮肤3~6月龄高于7，8月龄(P < 0.05)。胎儿颌面部皮肤及其他部位皮肤透明质酸含量均高于成人；各部位皮肤均呈现孕中、后期透明质酸含量下降，但下降时间不一致。

关键词: 胎儿, 颌面部, 皮肤, 透明质酸, 皮肤组织工程

Abstract:

BACKGROUND: High level of hyaluronic acid (HA) in extracellular matrix of wounds has been shown to inhibit scar formation and scarless healing has been observed in fetal wounds. However, there is little information regarding HA content in fetal skin.
OBJECTIVE: To determine HA content in maxillofacial skin, as well as the changing tendency, and compare with other regions of skin. 
METHODS: The fetal skin of the maxillofacial region, back, belly, and inner part of leg were harvested from 32 aborted fetuses ranging from 3 to 8 months. All the samples were frozen immediately by liquid nitrogen and preserved at -80 ℃. Different month groups were designated according to skin regions. The samples were weighed and cut into pieces, then grinded into tissue homogenate. HA content in supernatant liquid of all the samples was detected using radioimmunoassay.
RESULTS AND CONCLUSION: In 3-8 month-old fetuses, HA content was (303.85±80.28) μg/g in the maxillofacial skin, (308.09±61.17) μg/g in the belly skin, (276.38±58.52) μg/g in the back skin, and (304.27±60.51) μg/g in the leg skin. HA content in the maxillofacial and back skin was higher in 3-5 month-old fetuses than in 6-8 month-old fetuses (P < 0.05). HA content in the leg skin was higher in the 3-6 month-old fetuses than in the 7-8 month-old fetuses (P < 0.05). HA content in all parts of skin was higher in fetuses than in adults. In all parts, there was a decline of HA content in the mid- and late- stages of pregnancy, but the decline time differed.

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