中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2714-2717.doi: 10.3969/j.issn.1673-8225.2010.15.013

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

缝线诱导大鼠角膜血管新生过程中环氧化酶2和基质金属蛋白酶2的表达

张  静，陆晓和，张  妍，李  祥，钟彦彦，张海峰   

  1. 南方医科大学珠江医院眼科，广东省广州市 515282
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 陆晓和，博士，博士生导师，主任医师，教授，南方医科大学珠江医院眼科，广东省广州市 515282 ykdzjh@sina.com
  • 作者简介:张 静★，女，1982年生，山东省菏泽市人，汉族，南方医科大学在读硕士，主要从事角膜病的基础与临床研究。 zhangjing20072007@126.com

Expressions of cyclooxygenase-2 and matrix metalloproteinase-2 in suture-induced corneal neovascularization in rats

Zhang Jing, Lu Xiao-he, Zhang Yan, Li Xiang, Zhong Yan-yan, Zhang Hai-feng   

  1. Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou  515282, Guangdong Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Lu Xiao-he, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 515282, Guangdong Province, China ykdzjh@sina.com
  • About author:Zhang Jing★, Studying for master’s degree, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 515282, Guangdong Province, China zhangjing20072007@126.com

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被引次数

1

摘要：

背景：研究表明环氧化酶2和基质金属蛋白酶2在角膜新生血管的发生中起重要作用，但其具体机制及相互关系仍不明确。
目的：观察环氧化酶2和基质金属蛋白酶2在缝线法诱导的大鼠角膜新生血管中的表达，并分析其相关性，探讨角膜新生血管形成的有关机制。
方法：采用缝线法建立SD大鼠角膜新生血管模型，术后每日裂隙灯观察角膜新生血管的生长情况，并于1，4，7，14， 21 d取材，行免疫组化及RT-PCR检测环氧化酶2和基质金属蛋白酶2蛋白的分布及二者mRNA的相对表达量，并进行相关性分析。
结果与结论：角膜缝线后三四天可见明显从角膜缘伸入角膜的毛刷状小血管，垂直角膜缘切线方向，角膜缝线处水肿；7 d角膜新生血管生长旺盛，角膜水肿继续加重，14 d新生血管延伸到达或超过缝线位置，分支密集并互相吻合形成袢状血管；21 d角膜新生血管变细。免疫组化及RT-PCR显示环氧化酶2和基质金属蛋白酶2于缝线后表达逐渐增加，7 d 达高峰，以后随炎症细胞的减少而减弱，主要分布于炎症细胞胞质及新生血管内皮细胞，两种因子的表达在角膜新生血管中具有正相关性(r=0.981, P < 0.05)。证实炎症相关的角膜新生血管中环氧化酶2和基质金属蛋白酶2的表达增加，并且与新生血管的发生、发展密切相关。

关键词: 角膜新生血管, 环氧化酶2, 基质金属蛋白酶2, 眼科组织工程, 大鼠

Abstract:

BACKGROUND: Studies has demonstrated that cyclooxygenase-2 (COX-2) and matrix metalloproteinase-2 (MMP-2) may play an important role in corneal neovascularization (CNV), but their mechanisms and interactions remain unclear.  
OBJECTIVE: To investigate the expressions of COX-2 and MMP-2 in suture-induced CNV, and to analyze the interaction between COX-2 and MMP-2, additionally, to explore the mechanisms of CNV. 
METHODS: The models of CNV were induced by corneal stitch. The development of CNV was monitored daily under slit-lamp microscope. The expressions of COX-2 and MMP-2 and levels of COX-2 and MMP-2 mRNA in CNV were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) at days 1, 4, 7, 14 and 21 after operation, and their correlations were analyzed.
RESULTS AND CONCLUSION: New vessels appeared near corneal limbus and grew towards into transparent cornea at 3-4 days after stitching. The stitch sites were edematous. The neovascular thrived and edema increased at 7 days. The neovascular reached to stitch sites at 14 days, and then vascular net formed. Then vessels gradually became thinner at 21 days. Immunochemistry and RT-PCR demonstrated: the positive expressions of COX-2 and MMP-2 in CNV localized in inflammatory cells and endothelial cells of neovascularization progressed gradually after stitching, and involved the entire cornea at the 7 day post-treatment. After 7 days the expression decreased following the decrease of inflammatory cells. The expression of COX-2 was correlative with MMP-2 positively (r=0.981, P < 0.05). All results demonstrated that in inflammation-associated corneal angiogenesis, expression of COX-2 and MMP-2 progressed and closely related to the angiogenesis.

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