中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2688-2691.doi: 10.3969/j.issn.1673-8225.2010.15.007

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

构建关节软骨的滚压加载装置

张春秋1，2，樊瑜波1，孙明林3，武  汉4   

  1. 1北京航空航天大学生物与医学工程学院，北京市 100191；2天津理工大学机械工程学院，天津市300191；3解放军武警医学院附属医院，天津市 300161；4吉林大学中日联谊医院，吉林省长春市 130022
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 樊瑜波，教授，北京航空航天大学生物与医学工程学院，北京市 100191 yubofan@yahoo.com
  • 作者简介:张春秋☆，男，1967年生，吉林省抚松县人，汉族，2003年吉林大学毕业，博士，副教授，主要从事生物力学研究。 zchunqiu@sohu.com
  • 基金资助:

    国家自然科学基金(10872147)“滚动载荷用于功能化关节软骨构建的研究”项目；天津市自然科学基金项目“滚压载荷促进关节软骨功能化生长的研究(09JCYBJC1400)”；国家自然科学基金(30772209)“体外骨组织工程化培养中组织空心化现象机理的研究”。

A rolling depression loading device for articular cartilage construction

Zhang Chun-qiu 1,2 , Fan Yu-bo1, Sun Ming-lin3, Wu Han4   

  1. 1 School of Biological Science and Medical Engineering, Beihang University, Beijing  100191, China; 2 School of Mechanical Engineering, Tianjin University of Technology, Tianjin  300191, China; 3 Medical college of Chinese People’s Armed Police Force, Tianjin  300162, China; 4 China-Japan Friendship Hospital, Jilin University, Changchun  130025, Jilin Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Fan Yu-bo, Professor, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China yubofan@yahoo.com
  • About author:Zhang Chun-qiu☆, Doctor, Associate professor, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300191, China zchunqiu@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No. 10872147*, 30772209*; Natural Science Foundation of Tianjin, No. 09JCYBJC1400*

PDF

344

被引次数

13

摘要：

背景：通常使用的加载装置或反应器有灌流式生物反应器、液压系统、直接动态压缩装置、剪切系统、拉伸装置或其中部分的组合。以上每一种载荷单独作用都没有实现软骨功能化培养，但即使是这几种的组合也不足以表征软骨的力学状态，构建的软骨功能与天然软骨仍有较大差距。
目的：首次提出和研制了一种用于关节软骨构建的滚压加载装置，并在滚压加载条件下分析软骨的受力状态。
方法：该装置包括滚动控制系统和压缩调节机构。滚动控制系统通过步进电机、丝杠控制辊子滚动速度，由齿轮和齿条啮合形成辊子纯滚动，辊子能够以均匀速度滚过培养物。压缩调节机构通过楔形滑块的相对滑动可以保证工作面平行升降，从而可以对培养物产生均匀、可调的压缩量。
结果与结论：该装置对培养物提供了滚压的力学条件，使培养物处于动态压缩变形和剪切变形的复合加载过程。在滚压的力学条件下，有限元分析表明培养物受到复杂力学状态。培养物浅表层受到交替出现的压力、拉应力；中层在滚动方向平面内应力与表面有一定夹角，角度由-45°~+45°呈周期变化；深层处应力都为压应力，与3个坐标轴方向接近。该装置的加载运动方式与关节的运动方式一致，滚压加载可能有利于功能化关节软骨结构、功能的构建。

关键词: 关节软骨, 软骨组织工程, 滚压加载, 功能化构建, 数字化骨科

Abstract:

BACKGROUND: Hynamic mechanical loading devices or bioreactors contain perfusion bioreactor, hydraulic system, and dynamic compression device, shear system, stretching device or the combination of some devices. However, all these devices can not characterize the mechanical state of cartilage, and the function of constructed cartilage is far from natural cartilage.
OBJECTIVE: A novel loading device for articular engineering construction was firstly proposed, and to analyze the mechanical behavior of chondrocytes under this loading device.
METHODS: The rolling depression loading device comprised roll controlling system and compression-adjusting system. In roll controlling system, the roller could roll over tissue engineered constructs with uniform velocity via controlling stepper motor and gear. The compressive subsystem could produce uniform, controlled compressions by relatively sliding of two wedge blocks.
RESULTS AND CONCLUSION: Under the device, constructs would endure the dynamical compressive deformation and stress deformation. Finite element analysis showed that the rolling depression load provided constructs with complex mechanical conditions, namely, pressure-tensile stress presented alternately on the superficial zone of tissue engineered constructs, -45° to   + 45° periodic changed angles could be found between rolling-direction plane and surface in the mesopelagic zone, and compressive stress presented in the deep layer, which closely to the X,Y, Z-directions. The loading mode of this device is consistent with articular mode, thus, it may benefit the functional construction of engineered cartilage. 

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