中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2572-2578.doi: 10.3969/j.issn.1673-8225.2010.14.021

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

神经生长因子联合神经干细胞移植治疗大鼠脊髓损伤

范广明1，张文彬2，张  赛2，王丽君3   

  1. 1天津医科大学，天津市  300070；2武警医学院附属医院，天津市  300162；  3凌源市第一人民医院，辽宁省凌源市 122500
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 张 赛，博士，博士生导师，主任医师，武警医学院附属医院，天津市 300162 zhangsai718@yahoo.com
  • 作者简介:范广明★，男，1978年生，辽宁省喀左县人，蒙古族，天津医科大学在读硕士，主治医师，主要从事颅脑创伤亚低温治疗，脑肿瘤手术治疗和干细胞移植治疗。 Phoenix2a2@163.com

Nerve growth factor combined with neural stem cell transplantation for treating spinal cord injury in rats 

Fan Guang-ming1, Zhang Wen-bin2, Zhang Sai2, Wang Li-jun3   

  1. 1 Tianjin Medical University, Tianjin   300070, China; 2 Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin   300162, China; 3 First People’s Hospital, Lingyuan   122500, Liaoning Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Zhang Sai, Doctor, Doctoral supervisor, Chief physician, Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China zhangsai718@yahoo.com
  • About author:Fan Guang-ming★, Studying for master’s degree, Attending physician, Tianjin Medical University, Tianjin 300070, China Phoenix2a2@163.com

PDF

512

被引次数

60

摘要：

背景：单纯的神经干细胞移植对受损脊髓组织的修复作用并不理想，研究证实神经生长因子兼有神经元营养和促突起生长双重作用，可以有效的促进脊髓损伤后神经功能的恢复。
目的：观察神经干细胞移植联合应用神经生长因子对脊髓损伤后大鼠运动功能恢复的影响。
方法：SD大鼠42只，建立急性脊髓损伤模型后随机分成3组，伤后1周于损伤处分别注入培养液、单纯神经干细胞或神经干细胞联合神经生长因子。于伤后1，2，4，6，8周进行BBB评分和斜板实验等运动功能检测。伤后4周取材行病理切片苏木精-伊红染色及BrdU免疫组化染色，伤后8周取材行辣根过氧化物酶示踪观察及体感诱发电位观察神经电生理恢复情况。
结果与结论：伤后4周单纯神经干细胞组、神经干细胞联合神经生长因子组大鼠后肢运动功能均有较明显恢复，神经干细胞联合神经生长因子组较单纯神经干细胞组快，差异有显著性意义(P < 0.05)。培养液组亦有所恢复，但程度较轻。病理切片显示培养液组未见神经轴索通过。单纯神经干细胞组可见少量神经轴索样结构，神经干细胞联合神经生长因子组可见较多神经轴索样结构。BrdU的阳性细胞数及HRP阳性神经纤维数：神经干细胞联合神经生长因子组>单纯神经干细胞组>培养液组且各组之间差异有显著性意义(P < 0.01)。神经干细胞联合神经生长因子组大鼠体感诱发电位的潜伏期、波幅优于单纯神经干细胞组(P < 0.05)，明显优于培养液组(P < 0.01)。结果提示神经干细胞移植对于后肢功能的恢复有促进作用，联合应用神经生长因子有协同效果。

关键词: 脊髓损伤, 神经干细胞, 神经生长因子, 功能恢复, 大鼠

Abstract:

BACKGROUND: The effects of simple neural stem cell (NSC) transplantation on repair of damaged spinal cord are not ideal. Previous studies have shown that nerve growth factor (NGF) has both effects of neuron nutrition and process growth promotion, can effectively contribute to the recovery of neurofunction following spinal cord injury (SCI).
OBJECTIVE: To investigate the effect of neural stem cell transplantation combined with NGF application on the recovery of motor function of rats with SCI.
METHODS: A total of 42 Sprague Dawley rats were used to establish SPI models, and then divided randomly into three groups: medium, simple NSCs or NSCs + NGF. At 1 week following injury, medium, simple NSCs or NSCs + NGF were separately injected into damaged sites. At 1, 2, 4, 6, 8 weeks post-injury, all animals were evaluated on the hind limb behavior with Basso, Beattie and Bresnahan (BBB) locomotor rating scale and inclined plane test. At 4 weeks post-transplantation, histopathology hematoxylin-eosin staining and BrdU immunohistochemistry were performed. At 8 weeks post-transplantation, horseradish peroxidase (HRP) nerve trace and somatosensory evoked potential testing were performed to observe the recovery of nerve electrophysiology.
RESULTS AND CONCLUSION: At 4 weeks following injury, motor function of the rat hindlimb was significantly improved in the simple NSCs group and NSCs + NGF group. The recovery was rapid in the NSCs + NGF group than the simple NSCs group (P < 0.05). The recovery was slight in the medium group. Pathological sections demonstrated that neurite was not found in the medium group. A few neurites were seen in the simple NSCs group, and many neurites were observed in the NSCs + NGF group. Numbers of BrdU-positive cells and HRP-positive nerve fibers: NSCs + NGF group > simple NSCs group > medium group, there was significant difference among groups (P < 0.01). Latent period and amplitude of somatosensory evoked potentials in the NSCs + NGF group were superior to the simple NSCs group (P < 0.05), and significantly better than the medium group (P < 0.01). Results suggested that NSC transplantation can promote the recovery of hindlimb function, and combined with NGF presents synergic effect.

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