中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2513-2517.doi: 10.3969/j.issn.1673-8225.2010.14.009

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

超顺磁性氧化铁纳米粒子和CM-DiI荧光双标记骨髓间充质干细胞

窦兴葵1，郭  涛2，思永玉1，万林骏1，黄青青1，康  波2   

  1. 1昆明医学院第二附属医院麻醉科，云南省昆明市 650101；2昆明医学院第一附属医院心内科，云南省昆明市  650032
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 郭 涛，硕士，教授，昆明医学院第一附属医院心内科，云南省昆明市 650032 guotao20@hotmail.com
  • 作者简介:窦兴葵☆，女，1976年生，云南省昆明市人，汉族，昆明医学院在读博士，主治医师，主要从事围术期心血管疾病防治方面的研究。 douxk@126.com
  • 基金资助:

    云南省科技厅联合专项基金(2008CD007)，课题名称：体外心脏震波治疗对猪急性心肌梗死后心室重塑影响的实验研究。

Superparamagnetic iron oxide and chlormethylbenzamido-1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine labeling and tracing bone marrow mesenchymal stem cells 

Dou Xing-kui1, Guo Tao2, Si Yong-yu1, Wan Lin-jun1, Huang Qing-qing1, Kang Bo2   

  1. 1 Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical College, Kunming  650101, Yunnan Province, China; 2 Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming  650000, Yunnan Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Guo Tao, Master, Professor, Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China guotao20@hotmail.com
  • About author:Dou Xing-kui☆, Studying for doctorate, Attending physician, Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China douxk@126.com
  • Supported by:

    Specific Research Project of Yunnan Science and Technology Bureau, No. 2008CD007*

PDF

447

被引次数

19

摘要：

背景：细胞移植疗效监测取决于有效的标记方法，从而能够对移植细胞进行示踪。超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide，SPIO)是一种较为理想的新型磁共振对比剂，用SPIO标记细胞可实现对移植细胞进行活体示踪。而荧光活性染料CM-DiI无细胞毒性，不影响细胞的生长，适合标记和示踪细胞。
目的：观察SPIO及CM-DiI对骨髓间充质干细胞的标记及示踪效果。
方法：全骨髓法培养猪骨髓间充质干细胞，用含50 mg/L铁浓度的SPIO及CM-DiI标记骨髓间充质干细胞，将双标后的骨髓间充质干细胞经冠状动脉注入猪心肌梗死模型。4周后取心脏组织行冰冻切片观察。
结果与结论：SPIO及CM-DiI在体外标记骨髓间充质干细胞效率高，几乎达100%。经冠状动脉移植4周后心肌组织可找到双标记的骨髓间充质干细胞，结果提示SPIO及CM-DiI双标记骨髓间充质干细胞体内示踪效果好。

关键词: 超顺磁性氧化铁纳米粒子, 骨髓间充质干细胞, CM-DiI, 细胞示踪, 干细胞

Abstract:

BACKGROUND: The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo. Superparamagnetic iron oxide (SPIO) labeling is an ideal magnetic resonance contrast medium, and it offers the potential for non-invasive tracking of implanted cells. The chlormethylbenzamido-1,1' - dioctadecyl- 3, 3, 3',3' - tetramethylindocarbocyanine (CM-DiI) labeling does not have cytotoxicity, so it cannot influence cell growth.
OBJECTIVE: To investigate the effects of SPIO and CM-DiI labeling and tracking on bone marrow mesenchymal stem cells (BMSCs).
METHODS: Porcine BMSCs were isolated and cultured by the whole bone marrow method. BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-DiI. The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion. The frozen sections of the cardiac tissues were obtained after 4 weeks.
RESULTS AND CONCLUSION: Efficiency of SPIO and CM-DiI labeling BMSCs was nearly 100%. The SPIO and CM-DiI labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation. SPIO and CM-DiI labeling BMSCs were efficiently tracked in vivo.

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