中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (12): 2261-2264.doi: 10.3969/j.issn.1673-8225.2010.12.043

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

细菌纤维素膜作为创伤性敷料的可行性

马  霞1，张  华2，陈世文3   

  1. 1上海应用技术学院生物食品科学系，上海市  200235；2中国科学院上海有机化学研究所生命有机国家重点实验室，上海市  200023；3上海交通大学附属第六人民医院神经外科，上海市  200233
  • 出版日期:2010-03-19 发布日期:2010-03-19
  • 通讯作者: 陈世文，博士，上海交通大学附属第六人民医院神经外科，上海市 200233
  • 作者简介:马 霞☆，女，1971年生，山东省济南市人，汉族，2003年天津科技大学毕业，博士，教授，主要从事生物材料的制备及其应用。
  • 基金资助:

    山东省自然科学基金项目资助(Q2006F08) ；中国博士后科学基金项目资助(20080430659) ；上海应用技术学院人才引进项目(YJ2008-14)。

Feasibility of bacterial cellulose membrane as a wound dressing

Ma Xia1, Zhang Hua2, Chen Shi-wen3   

  1. 1 Department of Food Science & Bioengineering, Shanghai Institute of Technology, Shanghai  200235, China; 2 State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai  200023, China; 3 Department of Neurosurgery, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai  200233, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Chen Shi-wen, Doctor, Department of Neurosurgery, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China chenshiwen@126.com
  • About author:Ma Xia☆, Doctor, Professor, Department of Food Science & Bioengineering, Shanghai Institute of Technology, Shanghai 200235, China maxia0126@126.com
  • Supported by:

    Natural Science Foundation of Shandong Province, No. Q2006F08*; China Postdoctoral Scientific Program, No. 20080430659*; Shanghai Institute of Technology Program, No. YJ2008-14*

PDF

869

被引次数

22

摘要：

背景：细菌纤维素为纳米级纤维材料，具有良好的生物相容性、湿态时高的机械强度、良好的液体和气体透过性，有利于皮肤组织生长和限制感染，有可能成为一种临时皮肤代用品和有应用潜力的生物敷料。
目的：通过动物实验观察细菌纤维素膜作为创伤敷料的可能性。
方法：用自制创伤仪于大鼠背部脊柱两侧各制造2.0 cm×2.0 cm大小的皮肤伤口，深及真皮层，两个创面之间间隔1 cm。随机选择一侧为治疗组，表面敷以细菌纤维素膜，另一侧为对照组，敷以油纱布，用1号手术缝线缝扎固定于周围皮肤上。于术后4，7，14，21，28 d观察创面愈合情况，并进行光镜组织学观察。
结果与结论： ①所有实验动物无死亡。觅食饮水等活动正常，两组均未出现创面感染。②治疗组术后7，14，21，28 d伤口创面愈合率较对照组显著提高。③治疗组肉芽组织层形成较厚，成纤维细胞和血管内皮细胞增生活跃，胶原纤维束增多，真皮层内小血管增生明显。对照组肉芽组织层较薄，成纤维细胞和血管内皮细胞增生不活跃，胶原纤维含量较治疗组少，小血管数量少。结果提示细菌纤维素膜对皮肤创伤性损伤具有促进愈合和抗感染的作用。

关键词: 细菌纤维素, 生物敷料, 皮肤创伤, 大鼠, 纳米生物材料

Abstract:

BACKGROUND: Bacterial cellulose (BC) is a kind of nano-fiber material, characterized by excellent biocompatibility, high mechanical strength, good liquid and gas permeability in hygroscopic state, which is beneficial to skin growth and prevents infection. Thus, it may become a temporary skin substitute and a promising wound dressing.
OBJECTIVE: To study the possibility of applying BC membrane as wound dressing via animal experiment.
METHODS: Healthy Sprague-Dawley (SD) rats were adopted to make 2.0 cm × 2.0 cm square skin wounds around middle spinal cord on the back with a self-made wound meter, and the wounds reached the corium layer and there was a 1 cm interval between two wounds. One side treated with BC membrane was to serve as the treatment group, and the other side was treated with oil gauze as the control group. The dressing was fixed to the surrounding skins using surgical suture. The wound healing was observed at days 4, 7, 14, 21 and 28 after operation, and histological observation was performed under a light microscope.
RESULTS AND CONCLUSION:  ① No animal died, no wound infection occurred, and the activity of animals was normal during the experiment. ② Compared with the control group, the wound healing rate of the treatment group was remarkably improved at days 7, 14, 21 and 28 after operation. ③ In the treatment group, the granulation tissue was thicker, fibroblast and new blood capillary proliferated actively, collagen fibrous bundle appeared and small vessels proliferated. In the control group, the fibroblast and blood vessel endothelial cell proliferated inactively with few collagen fibrous bundle and small vessels. The results suggest that BC membrane facilitates wound healing and prevents infection following skin traumatic injury.

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