Preparation of N-hexane chitosan and its cytotoxicity and coagulation property

doi:10.3969/j.issn.2095-4344.0775

Abstract

Abstract:

BACKGROUND: Chitosan is a natural hemostatic material, but its hemostatic effect is limited and needs to be improved. Introducing alkyl groups into chitosan can modify its coagulation property. However, the coagulation mechanism of short alkyl groups that are grafted on chitosan powder is unclear until now.

OBJECTIVE: To study the cytotoxicity and coagulation property of the prepared N-hexane chitosan.

METHODS: N-hexane chitosan was prepared by reductive reaction and characterized by Fourier transform infrared spectroscopy and elemental analysis. Cytotoxicity of N-hexane chitosan was tested by MTT and fluorescence microscopy. Whole blood coagulation time, rheological properties and blood plasma coagulation time were evaluated for its coagulation effects.

RESULTS AND CONCLUSION: (1) A series of N-hexane chitosans with substitution degree of 8.67%, 18.06%, and 32.88% were synthesized successfully. (2) MTT results showed that the toxicity of N-hexane chitosan was graded 0 and 1. Fluorescence staining results showed that L929 cells grew well on the materials and dead cells were scarcely visible, indicating the low toxicity of the materials. (3) N-hexane chitosan could promote blood clotting, and its blood coagulation efficiency was increased with its substitution degree. No significant difference was found in activated partial thromboplastin time, prothrombin time and thrombin time of N-hexane chitosans with different substitution degrees. N-hexane chitosan could not promote activation of clotting factors. Its mechanism for accelerating coagulation needs in-depth investigations. N-hexane chitosan, compared with pure chitosan, shows better clotting effect, and this effect is enhanced with the increasing of the substitution degree.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Cytotoxicity Tests, Immunologic, Hemorheology, Hemostasis

CLC Number:

R318

Liu Meng-yuan, Jing Miao-lei, Guan Jing, Huang Shu-jie, Yang Jian, Li Zhi-hong. Preparation of N-hexane chitosan and its cytotoxicity and coagulation property[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(22): 3520-3526.

Figures/Tables 8

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