中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4541-4544.doi: 10.3969/j.issn.1673-8225.2011.24.042

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

鹿茸粉对股骨头缺血性坏死模型大鼠的治疗作用

帕力哈提•白克吐尔逊，彭  昊，李彬彬，黄  磊   

  1. 湖北省人民医院，湖北省武汉市  430060
  • 收稿日期:2010-12-02 修回日期:2011-01-25 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 彭昊，教授，硕士生导师，主要从事骨与关节疾病的研究。湖北省人民医院，湖北省武汉市 430060
  • 作者简介:帕力哈提?白克吐尔逊★，男，1983年生，新疆维吾尔自治区巩留县人，维吾尔族，武汉大学在读硕士，主要从事骨与关节疾病的研究。

Effects of antler powder on treatment of corticosteroid-induced avascular necrosis of the femoral head in rats

Pharhat Baktursun, Peng Hao, Li Bin-bin, Huang Lei   

  1. Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Received:2010-12-02 Revised:2011-01-25 Online:2011-06-11 Published:2011-06-11
  • Contact: Peng Hao, Professor, Master’s supervisor, Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China penghao5868@163.com
  • About author:Pharhat Baktursun★, Studying for master’s degree, Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China parhat0038@163. com

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被引次数

2

摘要：

背景：长期使用类固醇激素容易引起股骨头缺血性坏死，中药鹿茸具有骨生长因子，能促进伤口愈合、组织修复，可能对股骨头缺血性坏死具有治疗作用。
目的：观察鹿茸粉对大鼠激素诱导性股骨头缺血性坏死的治疗作用。
方法：将42只雄性wistar大鼠分成空白对照组、模型组和高、中、低剂量治疗组。1周2次臀部肌肉注射给予地塞米松(30 mg/kg)，连续6周。高、中、低剂量治疗组按200，400，800 mg/kg剂量灌胃鹿茸粉，每天1次，空白对照组、模型组给予同量生理盐水。治疗2个月后，测量大鼠血脂水平，取两侧股骨头测量骨密度、病理变化及血管内皮细胞生长因子表达。
结果与结论：类固醇激素干预后，大鼠造成明显股骨头缺血性坏死损伤，经过治疗后高、中、低剂量治疗组大鼠损伤骨组织明显恢复，与模型组相比，骨密度增高，总胆固醇、三酰甘油和低密度脂蛋白水平下降，股骨血管内皮细胞生长因子表达上升。提示鹿茸粉能通过增加骨形成，改善脂肪代谢和增加血管形成来治疗激素诱导性股骨头坏死。

关键词: 鹿茸粉, 股骨头缺血性坏死, 类固醇激素, 骨密度, 组织构建

Abstract:

BACKGROUND: Long-term utilization of steroid hormone easily induces avascular necrosis of the femoral head (ANFH). Cornu Cervi Pantotrichum possesses bone growth factor and can promote wound healing and tissue repairing, which may has effect on treatment of ANFH.  
OBJECTIVE: To investigate therapeutic effect of antler powder on ANFH induced by corticosteroids in rats.
METHODS: Totally 42 male wistar rats were randomly divided into the blank control, model and high-, moderate- and low-dose treatment groups. Exception those in the blank control group, all rats were intragluteally injected with dexamethasone at 30 mg/kg twice per week for 6 weeks to induce ANFH. Then the rats were treated with antler powder by oral gavage at 200, 400 and    800 mg/kg dosage, once per day for 60 days. Same volume of physiological saline was gavaged in the blank control and model groups. Serum lipid level and bone mineral density (BMD) of femur were measured, pathological change and expression of vascular endothelial growth factor (VEGF) were investigated.
RESULTS AND CONCLUSION: Steroid hormone intervention resulted in obviously ANFH in rats. After treatment with antler powder, the degree of necrosis was significant reduced. Compared with the model group, BMD were increased, total cholesterol, triacylglycerol, and low density lipoprotein levels were decreased, and the VEGF expression increased in the treatment groups. The results suggest that antler powder has a positive curative effect on ANFH by promoting bone formation, fat metabolism and increasing VEGF expression.

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