中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4473-4476.doi: 10.3969/j.issn.1673-8225.2011.24.025

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

高原低氧对大鼠心肺组织超微结构的影响

张春爱，李文华   

  1. 西藏民族学院，陕西省咸阳市 712082
  • 收稿日期:2011-01-06 修回日期:2011-04-15 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 李文华，女，汉族，1964年生，山西省大同市人，1987年西安医科大学毕业，副教授，主要从事高原细胞生物与遗传学的研究。西藏民族学院，陕西省咸阳市 712082 xzmylwh@163.com
  • 作者简介:张春爱，女，汉族，1964年生，陕西省三原县人，1988年西藏民族学院医学系毕业，主治医师，主要从事内科学的研究。 并列第一作者：李文华，女，汉族，1964年生，山西省大同市人，1987年西安医科大学毕业，副教授，主要从事高原细胞生物与遗传学的研究。西藏民族学院，陕西省咸阳市 712082 xzmylwh@163.com
  • 基金资助:

    课题受西藏自治区科技厅自然科学基金项目(2010)资助，人类血浆AVP基因多态性与高原习服的相关性及其影响因素研究。

High altitude hypoxia on the ultrastructure of heart and lung tissue

Zhang Chun-ai, Li Wen-hua   

  1. Tibet Nationalities Institute, Xianyang  712082, Shaanxi Province, China
  • Received:2011-01-06 Revised:2011-04-15 Online:2011-06-11 Published:2011-06-11
  • Contact: Li Wen-hua, Associate professor, Zhang Chun-ai, Attending physician, Tibet Nationalities Institute, Xianyang 712082, Shaanxi Province, China
  • About author:Zhang Chun-ai, Attending physician, Tibet Nationalities Institute, Xianyang 712082, Shaanxi Province, China xzmylwh@163.com Li Wen-hua, Associate professor, Zhang Chun-ai, Attending physician, Tibet Nationalities Institute, Xianyang 712082, Shaanxi Province, China Zhang Chun-ai and Li Wen-hua equally contributed to this work
  • Supported by:

    the Natural Science Foundation of Science and Technology Ministry of Tibet Autonomous Region, No.2010*

PDF

516

被引次数

18

摘要：

背景：研究表明快速进入高原地区时，机体不可避免地会受到不同程度的损伤，以心肺损伤较显著。
目的：观察低氧习服对高原低氧大鼠心肺组织的超微结构影响。
方法：将SD大鼠分别为进行高原低氧干预1，3和30 d，并设置对照组。3个高原低氧组由海拔5 m的西安途中耗时1 d带到海拔2 700 m的青海格尔木地区、途中耗时3，30 d分别带到海拔4 500 m的西藏那曲地区，观察各时间点心肺标本的组织学变化。
结果与结论：急性高原低氧1，3 d组肺组织显微和超微结构出现明显的间质性肺水肿和肺泡性肺水肿，其心脏组织光镜下大鼠各室壁心肌细胞均可见不同程度的浊肿、空泡变性、溶解坏死及间质水肿等，电镜下可见心肌细胞线粒体肿胀，肌浆网扩张，肌原纤维溶解，细胞内外水肿等，急性高原低氧3 d上述改变右室壁较左室壁明显，而低氧习服后高原低氧30 d组间质性水肿和则肺水肿明显减轻。结果证实，高原急性缺氧可造成大鼠间质性肺水肿和肺泡型肺水肿，并引起以右心室为主的全心性损伤，经过高原低氧习服后心肺组织病变明显减轻。

关键词: 习服, 高原低氧, 大鼠, 电镜, 超微结构, 组织工程

Abstract:

BACKGROUND: Studies show that, the body inevitably will be injured to different degrees upon the rapid access to high altitude, among them damage to heart and lung is apparent.
OBJECTIVE: To evaluate the influence of hypoxic acclimatization on the ultrastructure of rat heart and lung.
METHODS: SD rats were randomly divided into 4 groups, namely the plateau hypoxia 1, 3, 30 days, and a control group. The hypoxic groups of rats were brought from the Xi'an (elevation 5 m), to Golmud, Qinghai (elevation 2 700 m; 1 day), to the Naqu (elevation 4 500 m; time-consuming 3 and 30 days). The cardiopulmonary specimens were observed for the histological changes.
RESULTS AND CONCLUSION: At acute hypoxia 1 and 3 days, the microstructure and ultrastructure of lung tissues exhibited apparent interstitial pulmonary edema and alveolar pulmonary edema; rat ventricular myocytes showed varying degrees of cloudy swelling, vacuolar degeneration, necrosis and interstitial dissolved edema by the heart tissue observations under light microscopy; electron microscope displayed that, the mitochondria swelling, sarcoplasmic reticulum, myofibril dissolution, intracellular and extracellular edema in cardiac muscle cells. These changes in the right ventricular wall at hypoxia 3 days were more apparent compared with those in left ventricular wall. The interstitial edema after hypoxic acclimatization was significantly reduced at 30 days. High altitude acute hypoxia may induce pulmonary interstitial edema and alveolar pulmonary edema, as well as right ventricular-based heart damage. The heart and lung tissue lesions significantly reduced after high altitude hypoxic acclimatization.

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