中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4395-4399.doi: 10.3969/j.issn.1673-8225.2011.24.007

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

非酒精性脂肪肝模型小鼠的建立

王俊杰1，方会龙1，李纯伟1, 2，陈福春1，唐伟军1，蒋显勇1, 2，吴海燕3，陈东林1, 2   

  1. 1湘南学院，湖南省郴州市 423000
    2湘南学院附属医院，湖南省郴州市 423000
    3郴州市第一人民医院，湖南省郴州市 423000
  • 收稿日期:2011-03-19 修回日期:2011-05-16 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 陈东林，副教授，湘南学院王仙岭校区基础医学课部，湖南省郴州市 423000 cxyctl@126.com
  • 作者简介:王俊杰★，男，1977年生，湖南省郴州市人，汉族，2001年中南大学毕业，硕士，讲师，主要从事中药药理学研究。 towjjemail@126.com 并列第一作者：方会龙，女，1976年生，湖南省郴州市人，汉族，硕士，副教授，主要从事肝脏疾病防治研究。 huilongfang@163.com
  • 基金资助:

    湖南省教育厅课题资助项目：二氢杨梅素治疗非酒精性脂肪肝的研究(编号：10C1246)；郴州市科技计划项目：荷叶治疗非酒精性脂肪肝的活性成分分离及药效学研究(编号：2008gl03)；湖南省自然科学基金项目：新的细胞亚群Th17在血吸虫虫卵肉芽肿中的作用研究；湘南学院湖南省重点建设学科药理学及湖南省心脑血管天然药物研究重点实验室资助。

Establishment of non-alcoholic fatty liver model in mice

Wang Jun-jie1, Fang Hui-long1, Li Chun-wei1, 2, Chen Fu-chun1,Tang Wei-jun1, Jiang Xian-yong1, 2, Wu Hai-yan3, Chen Dong-lin1, 2   

  1. 1Xiangnan University, Chenzhou 423000, Hunan Province, China
    2Affiliated Hospital of Xiangnan University, Chenzhou 423000, Hunan Province, China
    3Chenzhou First People’s Hospital, Chenzhou 423000, Hunan Province, China
  • Received:2011-03-19 Revised:2011-05-16 Online:2011-06-11 Published:2011-06-11
  • Contact: Chen Dong-lin, Associate professor, Xiangnan University, Chenzhou 423000, Hunan Province, China; Affiliated Hospital of Xiangnan University, Chenzhou 423000, Hunan Province, China
  • About author:Wang Jun-jie★, Master, Lecturer, Xiangnan University, Chenzhou 423000, Hunan Province, China towjjemail@126.com Wang Jun-jie and Fang Hui-long contributed equally to this paper.
  • Supported by:

    a grant from Education Department of Hunan Province, No. 10C1246*; the Science and Technology Development Program of Chenzhou, No. 2008gl03*; the Natural Science Foundation of Hunan Province*; a grant from Key Laboratory of Cardio- cerebrovascular Natural Drugs of Hunan Province, Hunan Provincial Pharmacology Program of Xiangnan University*

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937

被引次数

32

摘要：

背景：以往非酒精性脂肪肝模型建立的常用诱导方法均有其局限性，因此，有必要建立高质量的非酒精性脂肪肝模型。
目的：拟利用复合高脂饮食和低浓度四氯化碳诱导小鼠非酒精性脂肪肝模型。
方法：取昆明小鼠随机分为3组：对照组，高脂模型组和高脂模型+四氯化碳组。观察饲养第2，4，6和8周末肝脏形态和病理变化，测定第8周末血清丙氨酸氨基转移酶，天冬氨酸氨基转移酶，胆固醇和三酰甘油，以及肝脏胆固醇和三酰甘油水平。
结果与结论：复合高脂饮食+5%四氯化碳腹腔注射的方式造模，第2周末开始出现炎症细胞浸润，第4周末出现少量脂滴，第6周末出现脂肪病变，第8周末在脂肪病变的基础上，部分小鼠出现了肝纤维化，8周末小鼠肝指数、血清丙氨酸氨基转移酶，天冬氨酸氨基转移酶，胆固醇和三酰甘油，肝脏胆固醇和三酰甘油浓度明显升高(P < 0.05或P < 0.01)。通过8周复合高脂饮食和5%四氯化碳腹腔注射成功建立小鼠非酒精性脂肪肝模型。

关键词: 非酒精性脂肪肝, 小鼠, 动物模型, 四氯化碳, 高脂饮食

Abstract:

BACKGROUND: Methods for inducing non-alcoholic fatty liver model (NAFLM) should be improved.
OBJECTIVE: To establish NAFLM in mice by high fat feeding and carbon tetrachloride.
METHODS: Kunming mice were randomly divided into three groups: control, high fat, and high fat plus carbon tetrachloride. Liver morphological and pathological changes were observed at 2, 4, 6 and 8 weeks. Serum levels of total cholesterol (TC), triglyceride (TG), alanine transaminase (ALT) and aspartate transaminase (AST) and hepatic TC, TG changes were determined.
RESULTS AND CONCLUSION: In high fat plus carbon tetrachloride group, inflammatory cell infiltration appeared in the mice liver after 2 weeks. There were a small amount of lipid droplets at 4 weeks, and steatosis emerged after 6 weeks. Hepatic fibrosis began to appear at 8 weeks. The serum levels of total TC, TG, ALT and AST, and hepatic TC, TG were significantly advanced in high fat plus carbon tetrachloride group compared with control group at 8 weeks (P < 0.05 or P < 0.01). The NAFLM can be established successfully by feeding with high fat food and injecting 5% carbon tetrachloride.

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