中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 2945-2948.doi: 10.3969/j.issn.1673-8225.2010.16.024

• 生物材料综述 biomaterial review • 上一篇    下一篇

微载体培养技术的研究与进展

周  燕1，刘宝林1，杨  波1，王  欣1，彭承宏2,3，韩宝三2,3   

  1. 1上海理工大学医疗器械与食品学院，生物热科学研究所，上海市  200093；2上海交通大学医学院附属瑞金医院普外科、乳腺疾病诊治中心，上海市 200025；3上海消化外科研究所，上海市 200025
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 韩宝三，博士，博士后，副主任医师，上海交通大学医学院附属瑞金医院普外科、乳腺疾病诊治中心，上海市 200025；上海消化外科研究所，上海市 200025 hanbaosan@126.com
  • 作者简介:周 燕★，女，1987年生，安徽省安庆市太湖县人，汉族，上海理工大学生物医学工程专业在读硕士，主要从事三维多孔微载体构建方面的研究。 zhou8713@126.com
  • 基金资助:

    国家自然科学基金项目(50776060)；教育部新世纪优秀人才计划项目(07-0559)；上海市东方学者资助计划项目；国家“十一五”高新技术研究发展计划－“863”项目(2008AA02Z417)；国家自然科学基金项目(30672043)；国家自然科学基金项目(30772105)；国家自然科学基金项目(20074031)；国家自然科学基金项目(30801107)；上海市教委科研创新项目(09YZ84)；上海市科委自然科学基金项目(07ZR14076)；山东省科技攻关计划项目(2008GG10002027)。

Research and progress of microcarrier culture technology

Zhou Yan1, Liu Bao-lin1, Yang Bo1, Wang Xin1, Peng Cheng-hong 2,3 , Han Bao-san 2,3   

  1. 1 Institute of Biothermal Engineering, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai  200093, China;2 Department of General Surgery, Diagnostic and Treating Center of Breast Cancer, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai  200025, China; 3 Shanghai Institute of Digestive Surgery, Shanghai 200025, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Han Bao-san, Doctor, Associate chief physician, Department of General Surgery, Diagnostic and Treating Center of Breast Cancer, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China; Shanghai Institute of Digestive Surgery, Shanghai 200025, China hanbaosan@126.com
  • About author:Zhou Yan★, Studying for master’s degree, Institute of Biothermal Engineering, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China zhou8713@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50776060*, 30672043*, 30772105*, 20074031*, 30801107*; Program for New Century Excellent Talents in University, No. 07-0559*; Oriental Scholar Program of Shanghai*; the State Plan for High-Tech Research and Development in Eleventh-five Year (National 863 Program), No. 2008AA02Z417*; Innovation Program of Shanghai Municipal Education Commission, No. 09YZ84*; the Natural Science Foundation of Shanghai Municipal Education Commission, No. 07ZR1407*; Shandong Province Science and Technology Research Program, No. 2008GG10002027*

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摘要：

背景：微载体培养技术是一种新兴的大规模细胞培养技术，广泛地应用于组织工程中种子细胞的扩增。微载体具有比表面积大等优点，在微载体培养技术中具有决定性作用。
目的：文章就近年来制备微载体的生物材料和方法的研究进展做一综述，为微载体培养技术和组织工程的研究提供理论基础。
方法：由第一作者于2009-10应用计算机检索PubMed数据库、万方数据库和维普数据库相关文献。英文资料的检索时间为1967/2009；中文资料的检索时间为1990/2009。英文检索词为“microcarrier，biomaterials cell culture, tissue engineering”；中文检索词为“微载体，生物材料，细胞培养，组织工程”。 纳入标准：①微载体材料、制备工艺及性能的研究。②微载体细胞培养的研究。③动物实验及临床应用。阅读标题和摘要进行筛选，共纳入34篇用于综述。
结果与结论：虽然国内外研究人员在微载体的研究与制备方面做了很多工作，但迄今仍与临床应用有一定的距离。目前的主要工作是将不同种类的材料通过新型的工艺制备得到复合材料，通过表面改性来调节微载体的力学和生物降解性能。

关键词: 微载体, 生物材料, 细胞培养, 制备工艺, 组织工程, 综述文献

Abstract:

BACKGROUND: Microcarrier culture technology has become a new and large scale cell culture technology. It has been mainly used in the amplification research of tissue engineering seed cells. Microcarrier possesses the advantage of larger surface area and plays an essential role in microcarrier culture technology.
OBJECTIVE: To sum up the biomaterials and methods of microcarrier preparation, and provide theoretical foundation for the study of microcarrier culture technology and tissue engineering.
METHODS: Articles were retrieved from PubMed, Wanfang, and VIP databases with the key words of “microcarrier, biomaterials cell culture, tissue engineering” in both English and Chinese between 1967/2009 and 1990/2009, respectively. Inclusion criteria: study addressing microcarrier materials, preparation, and performance; study of microcarrier cell culture; animal experiments and clinical applications. A total of 34 articles were originally retrieved based on their titles and abstracts.
RESULTS AND CONCLUSION: Although a lot of studies have reported research and preparation of microcarrier, clinical application remains still difficult. Recently, varying materials will be made into novel compound materials by new technology, which can adjust mechanics and biodegradation of microcarrier via surface modification.

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