中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (47): 8202-8208.doi: 10.3969/j.issn.2095-4344.2013.47.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

小肠黏膜下层与滑膜间充质干细胞的组织相容性

符培亮,张  雷,吴宇黎,吴海山,丛锐军,陈  松,丁喆如,周  琦   

  1. 上海市长征医院关节外科,上海市 200003
  • 修回日期:2013-08-28 出版日期:2013-11-19 发布日期:2013-11-19
  • 通讯作者: 吴宇黎,副主任医师,上海市长征医院关节外科,上海市 200003 wuyuli6019@189.cn
  • 作者简介:符培亮☆,男,1980年生,浙江省余姚市人,汉族,2009年解放军第二军医大学毕业,博士,主治医师,主要从事关节软骨修复研究。 fupeiliang@163.com 并列第一作者:张雷☆,男,1987年生,安徽省芜湖市人,汉族,解放军第二军医大学在读博士,讲师,主要从事关节软骨修复研究。 ra_eagle@hotmail.com
  • 基金资助:

    实验受“国家自然科学基金青年科学基金项目”资助,兔自体SMSCs修复重建半月板损伤的机制研究项目(81000798)*

Histocompatibility between small intestinal submucosa and synovial mesenchymal stem cells

Fu Pei-liang, Zhang Lei, Wu Yu-li, Wu Hai-shan, Cong Rui-jun, Chen Song, Ding Zhe-ru, Zhou Qi   

  1. Department of Joint Surgery, Changzheng Hospital of Shanghai, Shanghai  200003, China
  • Revised:2013-08-28 Online:2013-11-19 Published:2013-11-19
  • Contact: Wu Yu-li, Associate chief physician, Department of Joint Surgery, Changzheng Hospital of Shanghai, Shanghai 200003, China wuyuli6019@189.cn
  • About author:Fu Pei-liang☆, M.D., Attending physician, Department of Joint Surgery, Changzheng Hospital of Shanghai, Shanghai 200003, China fupeiliang@163.com Zhang Lei☆, Studying for doctorate, Lecturer, Department of Joint Surgery, Changzheng Hospital of Shanghai, Shanghai 200003, China ra_eagle@hotmail.com Fu Pei-liang and Zhang Lei contributed equally to this work.
  • Supported by:

    the National Natural Science Foundation of China for the Youth, No. 81000798*

摘要:

背景:小肠黏膜下层有抗微生物活性、良好的生物相容性及生物力学性能,能在体内快速降解,与半月板纤维软骨细胞的胞外基质相近。
目的:观察小肠黏膜下层与滑膜间充质干细胞是否有良好的组织相容性。
方法:先后采用物理方法与化学方法处理猪小肠黏膜下层,并进行苏木精-伊红染色和扫描电镜观察。制备小肠黏膜下层浸提液,行以下实验:①热源实验:在新西兰大白兔耳缘静脉注射小肠黏膜下层浸提液。②皮肤致敏实验:在新西兰大白兔背部皮内分别注射小肠黏膜下层浸提液、多聚甲醛溶液及生理盐水。③全身毒性实验:在新西兰大白兔耳缘静脉分别注射小肠黏膜下层浸提液与生理盐水。将小肠黏膜下层与成骨诱导的兔滑膜间充质干细胞共培养,以小肠黏膜下层单独培养为对照。
结果与结论:经物理处理过的小肠黏膜下层表面仍然存在小肠黏膜上皮细胞、脂肪细胞和一些其他细胞黏附;经化学方法处理后其残留的细胞数量明显减少,但主要结构和成分未被改变。小肠黏膜下层黏膜面较肌层面光滑,无细胞残留,表面纤维组织交错形成疏松的三维网状立体结构,孔隙率为80%。小肠黏膜下层是一种无毒、无刺激、无免疫原性,生物相容性很好的生物材料,与兔滑膜间充质干细胞具有良好的组织相容性。

关键词: 生物材料, 细胞外基质材料, 小肠黏膜下层, 滑膜间充质干细胞, 半月板, 组织工程, 组织相容性, 国家自然科学基金

Abstract:

BACKGROUND: Small intestinal submucosa is characterized as antimicrobial activity, good biocompatibility, bio-mechanical properties, and rapid degradation in vivo, similar to the extracellular matrix of meniscal fibrochondrocytes.
OBJECTIVE: To observe whether there exists a good histocompatibility between small intestinal submucosa and synovial mesenchymal stem cells.
METHODS: Small intestinal submucosa was treated with physical and chemical treatment. And hematoxylin-eosin staining and scanning electron microscopy observation were performed. Then, small intestinal submucosa extracts were prepared for the following experiments. (1) Pyrogenic test: small intestinal submucosa extracts were injected into the ear vein of New Zealand white rabbits. (2) Skin sensitization test: small intestinal submucosa extracts, paraformaldehyde solution and normal saline were respectively injected intradermally into New Zealand white rabbits. (3) General toxicity test: small intestinal submucosa extracts and normal saline were respectively injected into the ear vein of New Zealand white rabbits. Small intestinal submucosa was co-cultured with osteogenic rabbit synovial mesenchymal stem cells, and small intestinal submucosa cultured alone served as control.
RESULTS AND CONCLUSION: There were some intestinal mucosal epithelial cells, fat cells and other cells adhered onto the surface of small intestinal submucosa after physical treatment. While, the amount of residual cells decreased sharply after chemical treatment. But the main structure and the component had not been changed. The surface of small intestinal submucosa was smooth and no cells remained, and there was a three-dimension network spatial structure. The porosity was 80%. Small intestinal submucosa is a non-toxic, nonirritating, non-immunogenic biomaterial with very good biocompatibility, which has a good histocompatibility with rabbit synovial mesenchymal stem cells.

Key words: biocompatible materials, histocompatibility, bone matrix, extracellular matrix

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