中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (47): 8182-8188.doi: 10.3969/j.issn.2095-4344.2013.47.007

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

可塑形脱矿骨基质/透明质酸骨泥的制备与细胞相容性

张乃丽，张育敏，周  沫，杨  婷，王旭昇，马绍英，徐伟俊，董  丽，李宝兴   

  1. 中国辐射防护研究院，山西省医用组织库，山西省太原市  030006
  • 修回日期:2013-08-18 出版日期:2013-11-19 发布日期:2013-11-19
  • 通讯作者: 李宝兴，研究员，博士生导师，中国辐射防护研究院，山西省医用组织库，山西省太原市 030006 zymsir@sina.com
  • 作者简介:张乃丽☆，男，1980年生，山东省巨野县人，汉族，南方医科大学在读博士，助理研究员，主要从事医用生物材料的研究。 znl-0@163.com
  • 基金资助:

    中国辐射防护研究院青年科技基金资助项目(YQ120807)*

Preparation and cell compatibility of malleable demineralized bone matrix/hyaluronic acid putty

Zhang Nai-li, Zhang Yu-min, Zhou Mo, Yang Ting, Wang Xu-sheng, Ma Shao-ying, Xu Wei-jun, Dong Li,Li Bao-xing   

  1. Medical Tissue Bank, China Institute for Radiation Protection, Taiyuan  030006, Shanxi Province, China
  • Revised:2013-08-18 Online:2013-11-19 Published:2013-11-19
  • Contact: Li Bao-xing, Investigator, Doctoral supervisor, Medical Tissue Bank, China Institute for Radiation Protection, Taiyuan 030006, Shanxi Province, China zymsir@sina.com
  • About author:Zhang Nai-li☆, Studying for doctorate, Assistant investigator, Medical Tissue Bank, China Institute for Radiation Protection, Taiyuan 030006, Shanxi Province, China znl-0@163.com
  • Supported by:

     the Youth Science and Technology Foundation of China Institute for Radiation Protection, No. YQ120807*

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摘要：

背景：应用预制成形的材料修复不规则骨缺损时，常出现材料与骨缺损形状不匹配的问题，制备可任意塑形骨泥对促进不规则骨缺损的愈合具有重要意义。
目的：构建可塑形脱钙骨基质/透明质酸骨泥，筛选最佳复合比例并评价其细胞相容性。
方法：选取合格供体皮质骨制备脱钙骨基质。分别配置浓度为0.75%，1.5%，3%，4.5%的透明质酸溶液并检测其黏度，以450 mg脱钙骨基质与上述各浓度透明质酸溶液1 mL混合，制备不同比例的脱钙骨基质/透明质酸骨泥。37 ℃条件下将骨泥浸泡在PBS平衡液中，观察其离散时间，筛选最佳复合比例的脱钙骨基质/透明质酸骨泥。应用脱钙骨基质/透明质酸骨泥浸提液培养L-929小鼠成纤维细胞，1，4，7 d采用CCK-8法检测骨泥的细胞毒性。
结果与结论：随透明质酸浓度的增加其溶液黏度增大，脱钙骨基质/透明质酸骨泥的离散时间延长，3%浓度的透明质酸与450 mg脱钙骨基质复合制备的骨泥具有良好可塑形能力，离散时间为8 h，为最佳复合比例。脱钙骨基质/透明质酸骨泥浸提液培养L-929小鼠成纤维细胞1，4，7 d的细胞增殖率分别为93.72%，101.65%，97.68%，细胞毒性级别为0或1级。表明脱钙骨基质/透明质酸复合质量比为15∶1制备的骨泥具有良好的可塑形能力及抗离散能力，细胞毒性轻微。

关键词: 生物材料, 材料生物相容性, 骨组织构建, 可塑形骨泥, 脱钙骨基质, 透明质酸, 黏度, 抗离散能力, 细胞相容性, 骨修复材料, 其他基金

Abstract:

BACKGROUND: It is important to prepare a malleable putty to repair irregular bone defects because the preformed bone substitute material cannot suit to bone defects.
OBJECTIVE: To prepare malleable demineralized bone matrix/hyaluronic acid putty and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.
METHODS: Demineralized bone matrix was prepared from the cortical bone of healthy donors. The hyaluronic acid was dissolved at concentrations of 0.75%, 1.5%, 3%, 4.5%, and the viscosity of them was measured. Then 450 mg demineralized bone matrix was compounded with 1 mL hyaluronic acid solution to prepare the demineralized bone matrix/hyaluronic acid putty, and the optimal ratio of demineralized bone matrix/hyaluronic acid was screened by the disintegrated time of the putty at 37 ℃. L-929 mouse fibroblasts were cultured in the leaching liquor of demineralized bone matrix/hyaluronic acid, and the cytotoxicity was tested by cell count kit-8 assay at days 1, 4 and 7.
RESULTS AND CONCLUSION: With the increase of hyaluronic acid concentration, the viscosity of hyaluronic acid solution was increased; the demineralized bone matrix/hyaluronic acid putty was strengthened and the disintegrated time was prolonged gradually. When the ratio of demineralized bone matrix/hyaluronic acid was 15/1, demineralized bone matrix/hyaluronic acid was molded easily, and the disintegrated time was 8 hours in 7 ℃ PBS. The results of cytotoxicity showed that cell proliferation rates were 93.72%, 101.65% and 97.68% at days 1, 4 and 7 respectively, while the cytotoxicity was in grade 0 or 1. These findings indicate that demineralized bone matrix/hyaluronic acid putty at a compound ratio of 15:1 can be molded easily and difficult to disintegrate, with low cytotoxicity.

Key words: biocompatible materials, bone matrix, hyaluronic acid, histocompatibility testing

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