中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5076-5079.doi: 10.3969/j.issn.1673-8225.2011.27.030

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

β-淀粉样蛋白25~35和人参皂苷Rb1对神经干细胞分化过程中tau蛋白磷酸化水平的影响

赵庆霞1，刘小转2，李  博3，鄢文海3，韩雪飞3，邢  莹3   

  1. 1郑州大学护理学院，河南省郑州市   450053
    3郑州大学干细胞研究中心，河南省郑州市   450053
    2河南科技大学医学院，河南省洛阳市471000
  • 收稿日期:2011-01-26 修回日期:2011-04-25 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 邢莹，博士，教授，郑州大学基础医学院生理教研室，河南省郑州市450053 xingy@zzu.edu.cn
  • 作者简介:赵庆霞★，女，汉族，1975年生，河南省沁阳市人，2007年郑州大学毕业，硕士，主要从事干细胞的分化与调控方面的研究。 zzuzhaoqingxia@126.com 并列第一作者：刘小转,女，汉族，1978年生，河南省洛阳市人，2007年郑州大学毕业，硕士，主要从事干细胞的分化与调控方面的研究。 anya0609@126.com
  • 基金资助:

    河南省科技攻关基金资助项目(0424410085)，课题名称：脐血细胞诱导分化神经干细胞及其应用；河南省自然基金资助项目(0611043000)，课题名称：tau蛋白在脐血来源神经细胞发育中的作用和机制。

Effect of GinsenosideRb1 and beta-amyloid 25-35 on tau phosphorylation in differentiated neural stem cells

Zhao Qing-xia1, Liu Xiao-zhuan2, Li Bo3, Yan Wen-hai3, Han Xue-fei3, Xing Ying3   

  1. 1Nursing College of Zhengzhou University, Zhengzhou  450053, Henan Province, China; 3Stem Cells Research Center of Zhengzhou University, Zhengzhou  450053, Henan Province, China; 2Medical College, Henan University of Science and Technology, Zhengzhou  450053, Henan Province, China
  • Received:2011-01-26 Revised:2011-04-25 Online:2011-07-02 Published:2011-07-02
  • Contact: Xing Ying, Doctor, Professor, Stem Cells Research Center of Zhengzhou University, Zhengzhou 450053, Henan Province, China xingy@zzu.edu.cn
  • About author:Zhao Qing-xia★, Master, Nursing College of Zhengzhou University, Zhengzhou 450053, Henan Province, China zzuzhaoqingxia@126.com Liu Xiao-zhuan, Master, Medical College, Henan University of Science and Technology, Zhengzhou 450053, Henan Province, China anya0609@126.com Zhao Qing-xia and Liu Xiao-zhuan contributed equally to this paper.
  • Supported by:

    the Science and Technology Tackle Key Foundation of Henan Province, No. 0424410085*; the Natural Science Foundation of Henan Province, No. 0611043000*

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摘要：

背景：通过调节神经干细胞分化过程中的tau蛋白磷酸化水平可提高神经干细胞移植治疗阿尔茨海默病的效果。
目的：观察β-淀粉样蛋白25~35和人参皂苷Rb1对神经干细胞分化过程中tau蛋白磷酸化水平的影响。
方法：分离、培养新生大鼠海马神经干细胞，诱导第3代神经干细胞分化1周后，分别采用免疫荧光细胞化学和Western-blot检测Tau[pS396] 、Tau[pS262] 及GSK-3β[pTyr279，216]表达情况。
结果与结论：正常神经干细胞分化过程中有Tau[pS396] 和Tau[pS262]的表达，β-淀粉样蛋白25~35可以使Tau[pS396]、Tau[pS262]和GSK-3β[pT279/216]表达上调，而人参皂苷Rb1可以逆转β-淀粉样蛋白25~35的作用。提示β-淀粉样蛋白25~35和人参皂苷Rb1通过调节GSK-3β的活性对tau蛋白的磷酸化水平产生调控作用。

关键词: 人参皂苷Rb1, &beta, -淀粉样蛋白25~35, 神经干细胞, tau蛋白磷酸化, 大鼠, 海马

Abstract:

BACKGROUND: Currently, neural stem cells (NSCs) transplantation effect on the treatment of Alzheimer’s disease is improved by adjusting tau protein phosphorylation level during the differentiation of NSCs.
OBJECTIVE: To explore the effect of GinsenosideRb1 and beta-amyloid 25-35 (Aβ25-35) on tau phosphorylation after NSCs are transformed into neurons.
METHODS: NSCs were isolated and cultured from rat hippocampus. NSCs of the third passage were induced towards neurons after one week; the expression of Tau[pS396], Tau[pS262] and GSK-3β[pTyr279, 216] were tested by the immunofluorescent cytochemical staining and western-blot.
RESULTS AND CONCLUSION: The expression of Tau[pS396], Tau[pS262] and GSK-3β[pTyr279, 216] was found in the process of NSCs differentiation. Aβ25-35 could enhance their expression; moreover Ginsenoside Rb1 could reverse the effect of Aβ25-35. The results indicated that there was a certain level of phosphorylation in tau protein during NSCs differentiation. Aβ25-35 and GinsenosideRb1 could regulate the level of tau phosphorylation. The mechanism of its action is by adjusting GSK-3β activity.

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