中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4946-4950.doi: 10.3969/j.issn.1673-8225.2011.27.002

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

牙胚组织中可分离得到大量间充质干细胞

杨少光，邢  文，田  堃，刘  蒙，卢士红，赵钦军，任红英，池  颖，马凤霞，韩忠朝   

  1. 中国医学科学院北京协和医学院血液学研究所血液病医院，实验血液学国家重点实验室，天津市     300020
  • 收稿日期:2011-02-09 修回日期:2011-04-09 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 韩忠朝，中国医学科学院北京协和医学院血液学研究所血液病医院，实验血液学国家重点实验室，天津市 300020 tihzchan@public.tpt.tj.cn
  • 作者简介:杨少光，男，1953年生，重庆市人，汉族，1977年中山医科大学毕业，副主任技师，从事血液病相关造血干细胞和再造血微环境的研究。 yangsg666@163.com 并列第一作者：邢文★，男，1969年生，山东省莱阳市人，汉族，2004年上海第二医科大学毕业，硕士，助理研究员，主要从事实验血液学方面的研究。 xw2318@yahoo.com.cn
  • 基金资助:

    国家973项目(2011CB964800)，课题名称：造血干细胞维持、衰老与再生的调控机制研究。

A large mass of mesenchymal stem cells isolated from tooth germs

Yang Shao-guang, Xing Wen, Tian Kun, Liu Meng, Lu Shi-hong, Zhao Qin-jun, Ren Hong-ying, Chi Ying, Ma Feng-xia, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
  • Received:2011-02-09 Revised:2011-04-09 Online:2011-07-02 Published:2011-07-02
  • Contact: Han Zhong-chao, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China tihzchan@public.tpt.tj.cn
  • About author:Yang Shao-guang, Associate chief technician, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China yangsg666@163.com Xing Wen★, Master, Assistant researcher, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China xw2318@yahoo.com.cn Yang Shao-guang and Xing Wen contributed equally to this paper.
  • Supported by:

    the National 973 Program of China, No. 2011CB964800*

PDF

321

被引次数

4

摘要：

背景：已经有研究从牙髓、牙囊和牙周组织中分离得到间充质干细胞。牙胚是牙的前体，能否从中直接分离间充质干细胞，目前未知。
目的：从人流产胎儿牙胚中分离间充质干细胞，检测其生物学特征。
方法：无菌条件下挖出水囊流产胎儿牙床中的整个牙胚，剪碎后分别用胶原酶Ⅱ和胰酶消化，过滤除去颗粒物后，细胞悬液用培养液洗涤，培养、扩增得到贴壁细胞，进行细胞形态学观察、免疫表型测定和多向分化功能鉴定。
结果与结论：经过3次传代后，从单个胎儿牙胚中即可获得超过107形态均匀的、梭形贴壁细胞，其表达CD105、CD73、CD90和CD44等表面标志，不表达CD34、CD45标志；在体外向成骨、成脂分化，在体内可以分化为软骨细胞。提示用贴壁法可从人胎儿牙胚中分离得到大量的间充质干细胞。

关键词: 牙胚, 间充质干细胞, 人流产胎儿, 分离, 贴壁培养

Abstract:

BACKGROUND: Mesenchymal stem cells have been isolated from dental tissues such as the dental pulp, the dental follicle and the periodontal ligament. It is unclear whether mesenchymal stem cells can be isolated directly from tooth germs, the tooth precursors. 
OBJECTIVE: To isolate mesenchymal stem cells from tooth germs of aborted fetuses and to detect their biological characteristics.
METHODS: The tooth germs were dug out entirely from fetal alveolar ridge aseptically. They were cut into small pieces and digested with collagenase Ⅱ and trypsin successively. After filtering the pieces out, the cells were washed and cultured. The expanded adherent cells were studied. Cell morphology, immunophenotype and multi-potential differentiation were analyzed.
RESULTS AND CONCLUSION: After 3 passages, more than 107 homogenous, spindle-shaped adherent cells were obtained from one fetal tooth germ. These cells expressed the markers of CD105, CD73, CD90 and CD44, but they did not express CD34 and CD45. They could differentiate into osteoblasts and adipocytes in vitro, and chondrocytes in vivo. Mesenchymal stem cells can be isolated from fetal tooth germs by plastic adherence.

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