中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4951-4954.doi: 10.3969/j.issn.1673-8225.2011.27.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

兔骨髓间充质干细胞体外培养定向诱导分化为软骨细胞

俞  猛，于  方，付胜良   

  1. 首都医科大学附属北京世纪坛医院骨科，北京市100038
  • 收稿日期:2011-01-27 修回日期:2011-05-19 出版日期:2011-07-02 发布日期:2011-07-02
  • 作者简介:俞猛☆，男，1971年生，贵州省安顺市人，汉族，2006年华中科技大学同济医学院毕业，博士，主治医师，主要从事骨组织工程方面的研究。 yu_meng71@yahoo.com.cn

Differentiation of rabbit mesenchymal stem cells into chondrocytes in vitro

Yu Meng, Yu Fang, Fu Sheng-liang   

  1. Department of Orthopedics, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing  100038, China
  • Received:2011-01-27 Revised:2011-05-19 Online:2011-07-02 Published:2011-07-02
  • About author:Yu Meng☆, Doctor, Attending physician, Department of Orthopedics, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038, China yu_meng71@yahoo.com.cn

PDF

561

被引次数

24

摘要：

背景：骨髓间充质干细胞具有分化为骨、软骨、肌腱、脂肪等组织的多分化潜能。
目的：体外培养兔骨髓间充质干细胞并定向诱导分化为软骨细胞，探讨体外诱导分化为软骨的方法和条件。
方法：取兔股骨骨髓，全骨髓贴壁法分离培养骨髓间充质干细胞，碱性成纤维生长因子体外培养后，取第3代细胞在培养基中添加不同质量浓度转化生长因子β1的软骨分化诱导剂，2周后倒置显微镜观察细胞形态，甲苯胺蓝染色，RT-PCR技术检测Ⅱ型胶原和聚集蛋白聚糖的表达。
结果与结论：可以从兔骨髓中培养出骨髓间充质干细胞，碱性成纤维生长因子能明显的促进骨髓间充质干细胞增殖。经软骨诱导分化后骨髓间充质干细胞呈软骨细胞形态，甲苯胺蓝染色阳性，Ⅱ型胶原和聚集蛋白聚糖的表达阳性。10 μg/L的转化生长因子β1诱导成软骨分化能力最强。

关键词: 转化生长因子&beta, 1, 骨髓间充质干细胞, 碱性成纤维生长因子, 软骨分化, 兔

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have a multi-differentiated potential to differentiate into bone, cartilage, tendon, adipose and so on.
OBJECTIVE: To build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.
METHODS: BMSCs were isolated from young rabbit femur marrow, and then planted in plastic culture flask. After stimulated by basic fibroblast growth factor (bFGF), passage 3 cells were chosen to induce into cartilage differentiation in different concentrations of transforming growth factor-β1 (TGF-β1). Tow weeks later, the morphology of chondrocytes was detected by toludine blue, type Ⅱ collagen and aggrecan assay with RT-PCR.
RESULTS AND CONCLUSION: bFGF could stimulate the proliferation of BMSCs. Induced BMSCs had typical appearance of chondrocytes, the expression of type Ⅱ collagen and aggrecan were positive. 10 μg/L TGF-β1 was the optimal concentration for cartilage differentiation.

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