中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4312-4315.doi: 10.3969/j.issn.1673-8225.2011.23.030

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

表皮生长因子干预人羊膜间充质干细胞的增殖和迁移

李彩虹，施  萍，庞希宁   

  1. 中国医科大学细胞生物学卫生部重点实验室干细胞与再生医学研究室，辽宁省沈阳市  110001
  • 收稿日期:2011-01-04 修回日期:2011-03-11 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 庞希宁，博士，教授，中国医科大学细胞生物学卫生部重点实验室干细胞与再生医学研究室，辽宁省沈阳市 110001 pxining@yahoo.com
  • 作者简介:李彩虹，女，1987年生，山西省忻州市人，汉族，2009年山西大学毕业，主要从事羊膜间充质干细胞的增殖和迁移机制研究。 licaihong6812@126.com
  • 基金资助:

    沈阳市科学技术计划(2009-090063, 2011-F10-222-4-00)。 

Effect of epidermal growth factor on proliferation and migration of human amniotic mesenchymal stem cells

Li Cai-hong, Shi Ping, Pang Xi-ning   

  1. Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-01-04 Revised:2011-03-11 Online:2011-06-04 Published:2011-06-04
  • Contact: Pang Xi-ning, Doctor, Professor, Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China pxining@yahoo.com
  • About author:Li Cai-hong, Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China licaihong6812@126.com
  • Supported by:

    Shenyang Science and Technology Plan, No. 2009-090063, 2011-F10-222-4-00**

PDF

429

被引次数

14

摘要：

背景：人羊膜间充质干细胞移植可促进皮肤创伤的愈合。
目的：观察表皮生长因子对体外培养人羊膜间充质干细胞增殖和迁移的影响。
方法：人羊膜间充质干细胞经分离、鉴定并培养至第3代。细胞经过不同浓度的表皮生长因子处理后，用MTT法测量吸光度值从而计算出细胞存活率，通过计数穿过Transwell小室的细胞数测定表皮生长因子对细胞迁移能力的影响；并用表皮生长因子受体的抑制剂AG1478及PI3K的抑制剂LY294002检测其对细胞迁移能力的影响。
结果与结论：表皮生长因子处理后细胞的吸光度值升高1.55倍，穿过膜的数量增高1.5倍；AG1478、LY294002对表皮生长因子引起的迁移有抑制作用。提示表皮生长因子处理后细胞增殖和迁移能力均增强，迁移与EGFR-PI3K信号通路有关。

关键词: 人羊膜间充质干细胞, 表皮生长因子, 增殖, 迁移, 信号通路

Abstract:

BACKGROUND: Human amniotic mesenchymal stem cells (hAMSCs) have the ability to improve skin wound healing.
OBJECTIVE: To explore the effects of epidermal growth factor (EGF) on hAMSCs proliferation and migration in vitro. 
METHODS: Through isolation, identification and culture, the third generation of hAMSCs were obtained. After treatment with different concentrations of EGF, the absorbance was measure to calculate survival rate of the cells using MTT method. We also counted the number of cells through Transwell to determine the effect of EGF on cell migration. With inhibitors AG1478, LY294002 of EGFR and PI3K, we tested their influence on cell migration. 
RESULTS AND CONCLUSION: EGF caused 1.55 times increase in the absorbance, and 1.5 times increase in the number of cell across the film. So AG1478 and LY294002 had inhibitory effect on the migration hAMSCs treated with EGF. It is indicated that   the proliferation and migration ability of hAMSCs enhanced following treatment with EGF, and hAMSCs migration is related to PI3K signaling pathways.

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